Digitalni repozitorij raziskovalnih organizacij Slovenije

Statistika DiRROS
A+ | A- | SLO | ENG


Največkrat prenešene datoteke meseca (zadnjih 30 dni).

1.
Mast cell activation test in the diagnosis of allergic disease and anaphylaxis
Rajia Bahri, Adnan Custovic, Peter Korošec, Marina Tsoumani, Martin Barron, Jiakai Wu, Rebekah Sayers, Alf Weimann, Monica Ruiz-Garcia, Nandinee Patel, Mira Šilar, 2018

Opis: Background. Food allergy is an increasing public health issue and the commonest cause of life-threatening anaphylactic reactions. Conventional allergy tests assess for the presence of allergen-specific IgE, significantly overestimating the rate of true clinical allergy resulting in over-diagnosis and adverse impact on health-related quality of life. Objective. To undertake initial validation and assessment of a novel diagnostic tool, the mast cell activation test (MAT). Methods. Primary human mast cells (hMCs) were generated from peripheral blood precursors, and sensitized using patient sera and then incubated with allergen. Mast cell degranulation was assessed by flow cytometry and mediator release. We compared the diagnostic performance of MAT to existing diagnostic tools to assess in a cohort of peanut-sensitized individuals undergoing double-blind, placebo-controlled challenge. Results. hMCs sensitized with sera from peanut, grass pollen and hymenoptera- (wasp venom) allergic patients demonstrated allergen-specific and dose-dependent degranulation by both expression of surface activation markers (CD63 and CD107a) and functional assays (prostaglandins D2 and ß-hexosaminidase release). In this cohort of peanut-sensitized individuals, MAT was found to have superior discrimination performance compared to other testing modalities including component-resolved diagnostics and basophil activation test. Using functional principle component analysis, we identified 5 clusters or patterns of reactivity in the resulting dose-response curves, which at preliminary analysis corresponded to the reaction phenotypes seen at challenge. Conclusion. MAT is a robust tool which may confer superior diagnostic performance compared to existing allergy diagnostics, and may be useful to explore differences in effector cell function between basophils and mast cells during allergic reactions.
Ključne besede: allergy and immunology -- diagnosis, anaphylaxis, immunologic tests, mast cells, food hypersensitivity, basophil activation test, BAT, mast cell activation test
Objavljeno: 30.11.2020; Ogledov: 1643; Prenosov: 1554
.pdf Celotno besedilo (3,30 MB)
Prenosov v mesecu: 585

2.
Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathon
Zala Jan, Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drašler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič, 2021

Opis: Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
Ključne besede: membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon
Objavljeno: 25.01.2021; Ogledov: 1256; Prenosov: 1567
.pdf Celotno besedilo (1,88 MB)
Prenosov v mesecu: 561

3.
Analiza kakovosti drv na slovenskem trgu
Amina Gačo, Darja Stare, Urban Žitko, Peter Smolnikar, Peter Prislan, 2023

Ključne besede: drva, kakovost
Objavljeno: 12.01.2024; Ogledov: 194; Prenosov: 256
.pdf Celotno besedilo (6,24 MB)
Prenosov v mesecu: 213

4.
Objavljen prvi začasni standard FSC za gospodarjenje z gozdovi v Sloveniji
Amina Gačo, 2023

Ključne besede: standard za upravljanje z gozdovi, certifikat FSC, začasni standard
Objavljeno: 12.01.2024; Ogledov: 199; Prenosov: 213
.pdf Celotno besedilo (6,24 MB)
Prenosov v mesecu: 163

5.
PUR in geopolymer
Barbara Horvat, Nataša Knez, Uroš Hribar, Jakob Koenig, Branka Mušič, 2024

Opis: The dataset supports the results shown in the tables and figures in the article entitled “Thermal insulation and flammability of composite waste polyurethane foam encapsulated in geopolymer for sustainable building envelope” (doi: 10.1016/j.jclepro.2024.141387). It contains measurements of fire-behaviour characteristics, thermal conductivity, the behaviour of the material in the electromagnetic field in relation to the frequency, mechanical and structural evaluation, as well as chemical and mineralogical analysis.
Ključne besede: measurements, waste polyurethane foam, polymeric flame retardants, alkali activated material, metakaolin, microwave irradiation, thermal-fire behaviour, mechanical strength
Objavljeno: 20.02.2024; Ogledov: 173; Prenosov: 156
.xlsx Raziskovalni podatki (2,51 MB)
Prenosov v mesecu: 156

6.
Global impact of the COVID-19 pandemic on cytopathology practice : results from an international survey of laboratories in 23 countries
Elena Vigliar, Rima Cepurnaite, Eduardo Alcaraz-Mateos, Syed Z. Ali, Zubair W. Baloch, Claudio Bellevicine, Massimo Bongiovanni, Pavlina Botsun, Dario Bruzzese, Lukas Bubendorf, Izidor Kern, 2020

Opis: Background: To the authors' knowledge, the impact of the coronavirus disease 2019 (COVID-19) pandemic on cytopathology practices worldwide has not been investigated formally. In the current study, data from 41 respondents from 23 countries were reported. Methods: Data regarding the activity of each cytopathology laboratory during 4 weeks of COVID-19 lockdown were collected and compared with those obtained during the corresponding period in 2019. The overall number and percentage of exfoliative and fine-needle aspiration cytology samples from each anatomic site were recorded. Differences in the malignancy and suspicious rates between the 2 periods were analyzed using a meta-analytical approach. Results: Overall, the sample volume was lower compared with 2019 (104,319 samples vs 190,225 samples), with an average volume reduction of 45.3% (range, 0.1%-98.0%). The percentage of samples from the cervicovaginal tract, thyroid, and anorectal region was significantly reduced (P < .05). Conversely, the percentage of samples from the urinary tract, serous cavities, breast, lymph nodes, respiratory tract, salivary glands, central nervous system, gastrointestinal tract, pancreas, liver, and biliary tract increased (P < .05). An overall increase of 5.56% (95% CI, 3.77%-7.35%) in the malignancy rate in nongynecological samples during the COVID-19 pandemic was observed. When the suspicious category was included, the overall increase was 6.95% (95% CI, 4.63%-9.27%). Conclusions: The COVID-19 pandemic resulted in a drastic reduction in the total number of cytology specimens regardless of anatomic site or specimen type. The rate of malignancy increased, reflecting the prioritization of patients with cancer who were considered to be at high risk. Prospective monitoring of the effect of delays in access to health services during the lockdown period is warranted.
Ključne besede: pathology, neoplasms, COVID-19, coronavirus disease 2019, fine-needle aspiration, malignancy rate
Objavljeno: 09.11.2020; Ogledov: 3063; Prenosov: 1356
URL Povezava na datoteko
Prenosov v mesecu: 78

7.
Priročnik za učenje in igro v gozdu
2016

Ključne besede: elektronske knjige
Objavljeno: 12.07.2017; Ogledov: 7095; Prenosov: 5575
.pdf Celotno besedilo (9,56 MB)
Prenosov v mesecu: 71

8.
Odegós chréses gia te genetiké parakoloúthese dasón
2021

Ključne besede: gozdovi, genetski monitoring
Objavljeno: 28.05.2021; Ogledov: 1590; Prenosov: 1924
.pdf Celotno besedilo (14,95 MB)
Prenosov v mesecu: 68

9.
Thermal insulation and flammability of composite waste polyurethane foam encapsulated in geopolymer for sustainable building envelope
Barbara Horvat, Nataša Knez, Uroš Hribar, Jakob Koenig, Branka Mušič, 2024

Opis: Polyurethane foam (PUR) is a lightweight, thermally insulating, widely used, and highly flammable material that has after its use an adverse effect on the environment, i.e., PUR disposal is considered hazardous. Its flammability can be mitigated using various fire retardants, but they do not change the hazardous nature of waste PUR. Therefore, in the current study, waste PUR with and without flame retardants based on N and P was incorporated into a geopolymer, the alkali-activated material (AAM) based solely on metakaolin, to evaluate the potential recycling route of waste PUR while taking into account its flammability, so it can enter safely into the circular economy through the building industry. To enhance the mechanical properties of the composite, a fresh mixture was irradiated with microwaves. However, the irradiation of geopolymer containing PUR negatively influenced mechanical performance, which led to the evaluation of the behaviour of the complex dielectric constant of PUR and fire retardants. Materials and composites were evaluated regarding their chemistry, mineralogy, microstructure, and porosity to connect the structure with extrinsic properties like geometrical density, thermal conductivity, and fire properties. Nonetheless, positive influences of PUR being encapsulated in the geopolymer were lowered density (from 1.8 to 1.6 kg/l) and improved thermal insulation ability (from 940 to 860 mW/(m·K)) of the composites: with the inclusion of <5 % of PUR, thermal insulation improved by nearly 10 %. However, the contribution of PUR to the composite originated from its skeleton, which has more than 15 times bigger geometrical density (0.81 kg/l) compared to the density of the skeleton (0.047 kg/l). This offers an open field for further advancements of thermal properties, but would also lead to a decrease of the compressive strength, which was already lowered from 90 MPa for 30 % with <5 % of added grated PUR. Furthermore, the flammable nature of PUR and its other drawbacks can be controlled by permanent embedding in the noncombustible structure of geopolymer, making the envelope of sustainable buildings green and safer. Overall, including grated waste PUR in geopolymer represents a promising, easy, cost-effective recycling path with low energy consumption, where the composite cannot develop fire on a scale of pure PUR, even in the worst-case scenario, but only if the composite is designed in a way, that flammable materials cannot join flames during their combustion. This paper gives prospects to other flammable waste materials to be safely used in the circular economy, and to porous materials to shape properties of the composite by their intrinsic and/or extrinsic properties.
Ključne besede: waste polyurethane foam, polymeric flame retardants, alkali activated material, metakaolin, microwave irradiation, thermal-fire behaviour, mechanical strength
Objavljeno: 01.03.2024; Ogledov: 141; Prenosov: 117
.pdf Celotno besedilo (29,74 MB)
Prenosov v mesecu: 67

10.
Kamnolom apnenca v Podpeči pri Ljubljani in njegovi izdelki
Bojan Djurić, Luka Gale, Rok Brajkovič, 2022

Opis: Kamnolom v naselju Podpeč pri Ljubljani velja za glavni kamnolom rimske kolonije Emone (Ljubljana). Obstoj antič-nega kamnoloma v Podpeči je bil do sedaj utemeljen le na podlagi makroskopske podobnosti med Členom litiotidnega apnenca, ki izdanja v Podpeči, in z njim povezanimi rimskimi spomeniki, odkritimi v Ljubljani. V okviru pričujočega dela smo skušali poiskati oprijemljive arheološke in geoarheološke dokaze za njegov obstoj. V skrajno severnem delu kamnoloma smo izvedli arheološko sondiranje, hkrati pa natančno določili sestavo apnenca na tem območju in v 288 izdelkih iz antične Emone. Sondiranje severno od modernega kamnoloma je odkrilo dobro ohranjene sledove rimsko-dobne kamnoseške dejavnosti. Hkrati je litološka analiza spomenikov iz Emone razkrila, da je kar 182 (ali 64 odstotkov) analiziranih spomenikov izdelanih iz apnenca, litološko identičnega različkom, ki izdanjajo v Podpeči. Ti različki se prostorsko grupirajo severno od modernega kamnoloma. Pri tem sicer ostaja odprto vprašanje pripadnosti nekaterih različkov apnenca v izdelkih, ki so brez diagnostičnih komponent in bi lahko pripadali tudi nekoliko starejšemu Členu krkinega apnenca. Rekonstrukcijo obsega starega kamnoloma omogoča primerjava faciesov izvornih litostratigrafskih enot in izdelkov, pa tudi historična analiza zgodnjih kartografskih in katastrskih dokumentov. Analiza najzgodnejših izdelkov je omogočila hipotezo o prihodu kamnosekov iz kamnoloma v Nabrežini.
Ključne besede: Slovenija, Podpeč, Emona, rimska doba, litiotidni apnenec, mikrofaciesna analiza, rimski in novoveški kamnolom, izdelki
Objavljeno: 21.07.2022; Ogledov: 631; Prenosov: 522
.pdf Celotno besedilo (28,23 MB)
Prenosov v mesecu: 66