| Naslov: | Determination of silver(I)-binding sites in canonical B-DNA by NMR spectroscopy |
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| Avtorji: | ID Lenz, Tabea (Avtor)
ID Javornik, Uroš (Avtor)
ID Hebenbrock, Marian (Avtor)
ID Plavec, Janez (Avtor)
ID Müller, Jens (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://link.springer.com/article/10.1007/s00775-025-02115-y
 PDF - Predstavitvena datoteka, prenos (2,11 MB)
MD5: 983413B60BCD333D5E60012C10B492DD
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  KI - Kemijski inštitut
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| Povzetek: | The interaction of metal ions with nucleic acids was studied by determining the initial binding sites of Ag+ ions at unmodified B-DNA by NMR spectroscopy. In particular, NMR spectra were recorded of the Dickerson-Drew dodecamer sequence in the presence of different ratios of Ag+ ions to DNA. The data indicate that the coordination of the first three Ag+ ions per duplex preferentially takes place inside the B-DNA helix rather than at other possible binding sites such as the negatively charged phosphate backbone and/or the endocyclic N7 position of purine residues. Larger DNA aggregates are formed in the presence of excess Ag+ ions, as indicated by the formation of a precipitate and by significant changes in the circular dichroism spectra. As shown by a titration with chloride ions, the Ag+ ions are only loosely bound to the nucleic acids and can be released by precipitation of AgCl. |
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| Ključne besede: | biochemistry, DNA, spectroscopy, NMR |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 10.07.2025 |
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| Leto izida: | 2025 |
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| Št. strani: | str. 381–395 |
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| Številčenje: | Vol. 30 |
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| PID: | 20.500.12556/DiRROS-23531  |
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| UDK: | 577 |
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| ISSN pri članku: | 1432-1327 |
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| COBISS.SI-ID: | 247866115 |
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| Avtorske pravice: | © The Author(s) 2025 |
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| Opomba: | Nasl. z nasl. zaslona;
Soavtorja iz Slovenije: Uroš Javornik, Janez Plavec;
Opis vira z dne 5. 9. 2025;
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| Datum objave v DiRROS: | 08.09.2025 |
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| Število ogledov: | 271 |
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| Število prenosov: | 102 |
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| Metapodatki: |    |
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