20.500.12556/DiRROS-23531 Determination of silver(I)-binding sites in canonical B-DNA by NMR spectroscopy The interaction of metal ions with nucleic acids was studied by determining the initial binding sites of Ag+ ions at unmodified B-DNA by NMR spectroscopy. In particular, NMR spectra were recorded of the Dickerson-Drew dodecamer sequence in the presence of different ratios of Ag+ ions to DNA. The data indicate that the coordination of the first three Ag+ ions per duplex preferentially takes place inside the B-DNA helix rather than at other possible binding sites such as the negatively charged phosphate backbone and/or the endocyclic N7 position of purine residues. Larger DNA aggregates are formed in the presence of excess Ag+ ions, as indicated by the formation of a precipitate and by significant changes in the circular dichroism spectra. As shown by a titration with chloride ions, the Ag+ ions are only loosely bound to the nucleic acids and can be released by precipitation of AgCl. biochemistry DNA spectroscopy NMR biokemija DNA spektroskopija NMR true false true Angleški jezik Slovenski jezik © The Author(s) 2025 Neznano 2025-09-08 12:06:10 2025-09-08 12:16:15 2025-10-24 03:54:30 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorja iz Slovenije: Uroš Javornik, Janez Plavec; Opis vira z dne 5. 9. 2025; str. 381–395 Vol. 30 10. Jul. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-07-10 577 1432-1327 247866115 https://link.springer.com/article/10.1007/s00775-025-02115-y 1 73d24a6b-8c9b-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34808 RAZ_Lenz_Tabea_2025.pdf RAZ_Lenz_Tabea_2025.pdf 1 983413B60BCD333D5E60012C10B492DD 6ce9ae004c022e52e1ddce726df061fb0723663f2f6f6eea6a71de0d0940f9fd 9e59123b-8c9c-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34809 Kemijski inštitut 0 0 0