| Title: | Determination of silver(I)-binding sites in canonical B-DNA by NMR spectroscopy |
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| Authors: | ID Lenz, Tabea (Author)
ID Javornik, Uroš (Author)
ID Hebenbrock, Marian (Author)
ID Plavec, Janez (Author)
ID Müller, Jens (Author) |
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| Files: |  URL - Source URL, visit https://link.springer.com/article/10.1007/s00775-025-02115-y
 PDF - Presentation file, download (2,11 MB)
MD5: 983413B60BCD333D5E60012C10B492DD
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  KI - National Institute of Chemistry
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| Abstract: | The interaction of metal ions with nucleic acids was studied by determining the initial binding sites of Ag+ ions at unmodified B-DNA by NMR spectroscopy. In particular, NMR spectra were recorded of the Dickerson-Drew dodecamer sequence in the presence of different ratios of Ag+ ions to DNA. The data indicate that the coordination of the first three Ag+ ions per duplex preferentially takes place inside the B-DNA helix rather than at other possible binding sites such as the negatively charged phosphate backbone and/or the endocyclic N7 position of purine residues. Larger DNA aggregates are formed in the presence of excess Ag+ ions, as indicated by the formation of a precipitate and by significant changes in the circular dichroism spectra. As shown by a titration with chloride ions, the Ag+ ions are only loosely bound to the nucleic acids and can be released by precipitation of AgCl. |
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| Keywords: | biochemistry, DNA, spectroscopy, NMR |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 10.07.2025 |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 381–395 |
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| Numbering: | Vol. 30 |
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| PID: | 20.500.12556/DiRROS-23531  |
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| UDC: | 577 |
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| ISSN on article: | 1432-1327 |
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| COBISS.SI-ID: | 247866115 |
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| Copyright: | © The Author(s) 2025 |
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| Note: | Nasl. z nasl. zaslona;
Soavtorja iz Slovenije: Uroš Javornik, Janez Plavec;
Opis vira z dne 5. 9. 2025;
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| Publication date in DiRROS: | 08.09.2025 |
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| Views: | 275 |
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| Downloads: | 102 |
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