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Naslov:The digital MIQE guidelines update : minimum information for publication of quantitative digital PCR experiments for 2020
Avtorji:ID Huggett, Jim F. (Avtor)
ID Whale, Alexandra S. (Avtor)
ID Spiegelaere, Ward De (Avtor)
ID Abdel Nour, Afif M. (Avtor)
ID Bae, Young-Kyung (Avtor)
ID Beneš, Vladimír (Avtor)
ID Burke, Dan (Avtor)
ID Cleveland, Megan (Avtor)
ID Corbisier, Philippe (Avtor)
ID Devonshire, Alison S. (Avtor)
ID Dong, Lianhua (Avtor)
ID Drandi, Daniela (Avtor)
ID Foy, Carole A. (Avtor)
ID Garson, Jeremy A. (Avtor)
ID He, Hua-Jun (Avtor)
ID Hellemans, Jan (Avtor)
ID Kubista, Mikael (Avtor)
ID Lievens, Antoon (Avtor)
ID Makrigiorgos, Mike G. (Avtor)
ID Milavec, Mojca (Avtor)
ID Mueller, Reinhold D. (Avtor)
ID Nolan, Tania (Avtor)
ID O'Sullivan, Denise M. (Avtor)
ID Pfaffl, Michael W. (Avtor)
ID Rödiger, Stefan (Avtor)
ID Romsos, Erica L. (Avtor)
ID Shipley, Gregory L. (Avtor)
ID Taly, Valérie (Avtor)
ID Untergasser, Andreas (Avtor)
ID Wittwer, Carl T. (Avtor)
ID Bustin, Stephen A. (Avtor)
ID Vandesompele, Jo (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://academic.oup.com/clinchem/article/66/8/1012/5880117
 
.pdf PDF - Predstavitvena datoteka, prenos (1,44 MB)
MD5: 23381B7B5F978EBD283B244E41BF8AD5
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1093/clinchem/hvaa125
 
Jezik:Angleški jezik
Tipologija:1.02 - Pregledni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:03.08.2020
Leto izida:2020
Št. strani:str. 1012-1029
Številčenje:Vol. 66, iss. 8
PID:20.500.12556/DiRROS-20120 Novo okno
UDK:577
ISSN pri članku:0009-9147
DOI:10.1093/clinchem/hvaa125 Novo okno
COBISS.SI-ID:24618243 Novo okno
Datum objave v DiRROS:06.08.2024
Število ogledov:504
Število prenosov:393
Metapodatki:XML DC-XML DC-RDF
:
HUGGETT, Jim F., WHALE, Alexandra S., SPIEGELAERE, Ward De, ABDEL NOUR, Afif M., BAE, Young-Kyung, BENEŠ, Vladimír, BURKE, Dan, CLEVELAND, Megan, CORBISIER, Philippe, DEVONSHIRE, Alison S., DONG, Lianhua, DRANDI, Daniela, FOY, Carole A., GARSON, Jeremy A., HE, Hua-Jun, HELLEMANS, Jan, KUBISTA, Mikael, LIEVENS, Antoon, MAKRIGIORGOS, Mike G., MILAVEC, Mojca, MUELLER, Reinhold D., NOLAN, Tania, O’SULLIVAN, Denise M., PFAFFL, Michael W., RÖDIGER, Stefan, ROMSOS, Erica L., SHIPLEY, Gregory L., TALY, Valérie, UNTERGASSER, Andreas, WITTWER, Carl T., BUSTIN, Stephen A. in VANDESOMPELE, Jo, 2020, The digital MIQE guidelines update : minimum information for publication of quantitative digital PCR experiments for 2020. Clinical chemistry [na spletu]. 2020. Vol. 66, no. 8, p. 1012–1029. [Dostopano 6 april 2025]. DOI 10.1093/clinchem/hvaa125. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=20120
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Gradivo je del revije

Naslov:Clinical chemistry
Skrajšan naslov:Clin Chem
Založnik:P.B. Hoeber
ISSN:0009-9147
COBISS.SI-ID:5865479 Novo okno

Gradivo je financirano iz projekta

Financer:EC - European Commission
Številka projekta:733112
Naslov:SPIDIA for Personalized Medicine - Standardisation of generic Pre-analytical procedures for In-vitro DIAgnostics for Personalized Medicine
Akronim:SPIDIA4P
Financer:EC - European Commission
Številka projekta:115749
Naslov:Cancer treatment and monitoring through identification of circulating tumour cells and tumour related nucleic acids in blood
Akronim:CANCER-ID
Financer:Drugi - Drug financer ali več financerjev
Program financ.:UK Government Department of Business, Energy and Industrial Strategy, RVO
Številka projekta:86652036
Financer:Drugi - Drug financer ali več financerjev
Program financ.:ERDF
Številka projekta:CZ.1.05/1.1.00/02.0109
Akronim:BIOCEV

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:biokemija, digitalna PCR, dPCR, MIQE, dMIQE


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