| Title: | The digital MIQE guidelines update : minimum information for publication of quantitative digital PCR experiments for 2020 |
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| Authors: | ID Huggett, Jim F. (Author)
ID Whale, Alexandra S. (Author)
ID Spiegelaere, Ward De (Author)
ID Abdel Nour, Afif M. (Author)
ID Bae, Young-Kyung (Author)
ID Beneš, Vladimír (Author)
ID Burke, Dan (Author)
ID Cleveland, Megan (Author)
ID Corbisier, Philippe (Author)
ID Devonshire, Alison S. (Author)
ID Dong, Lianhua (Author)
ID Drandi, Daniela (Author)
ID Foy, Carole A. (Author)
ID Garson, Jeremy A. (Author)
ID He, Hua-Jun (Author)
ID Hellemans, Jan (Author)
ID Kubista, Mikael (Author)
ID Lievens, Antoon (Author)
ID Makrigiorgos, Mike G. (Author)
ID Milavec, Mojca (Author)
ID Mueller, Reinhold D. (Author)
ID Nolan, Tania (Author)
ID O'Sullivan, Denise M. (Author)
ID Pfaffl, Michael W. (Author)
ID Rödiger, Stefan (Author)
ID Romsos, Erica L. (Author)
ID Shipley, Gregory L. (Author)
ID Taly, Valérie (Author)
ID Untergasser, Andreas (Author)
ID Wittwer, Carl T. (Author)
ID Bustin, Stephen A. (Author)
ID Vandesompele, Jo (Author) |
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| Files: |  URL - Source URL, visit https://academic.oup.com/clinchem/article/66/8/1012/5880117
 PDF - Presentation file, download (1,44 MB)
MD5: 23381B7B5F978EBD283B244E41BF8AD5
URL - Source URL, visit https://doi.org/10.1093/clinchem/hvaa125
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| Language: | English |
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| Typology: | 1.02 - Review Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology. |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 03.08.2020 |
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| Year of publishing: | 2020 |
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| Number of pages: | str. 1012-1029 |
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| Numbering: | Vol. 66, iss. 8 |
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| PID: | 20.500.12556/DiRROS-20120  |
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| UDC: | 577 |
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| ISSN on article: | 0009-9147 |
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| DOI: | 10.1093/clinchem/hvaa125 |
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| COBISS.SI-ID: | 24618243 |
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| Publication date in DiRROS: | 06.08.2024 |
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| Views: | 337 |
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| Downloads: | 260 |
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