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Title:The digital MIQE guidelines update : minimum information for publication of quantitative digital PCR experiments for 2020
Authors:ID Huggett, Jim F. (Author)
ID Whale, Alexandra S. (Author)
ID Spiegelaere, Ward De (Author)
ID Abdel Nour, Afif M. (Author)
ID Bae, Young-Kyung (Author)
ID Beneš, Vladimír (Author)
ID Burke, Dan (Author)
ID Cleveland, Megan (Author)
ID Corbisier, Philippe (Author)
ID Devonshire, Alison S. (Author)
ID Dong, Lianhua (Author)
ID Drandi, Daniela (Author)
ID Foy, Carole A. (Author)
ID Garson, Jeremy A. (Author)
ID He, Hua-Jun (Author)
ID Hellemans, Jan (Author)
ID Kubista, Mikael (Author)
ID Lievens, Antoon (Author)
ID Makrigiorgos, Mike G. (Author)
ID Milavec, Mojca (Author)
ID Mueller, Reinhold D. (Author)
ID Nolan, Tania (Author)
ID O'Sullivan, Denise M. (Author)
ID Pfaffl, Michael W. (Author)
ID Rödiger, Stefan (Author)
ID Romsos, Erica L. (Author)
ID Shipley, Gregory L. (Author)
ID Taly, Valérie (Author)
ID Untergasser, Andreas (Author)
ID Wittwer, Carl T. (Author)
ID Bustin, Stephen A. (Author)
ID Vandesompele, Jo (Author)
Files:URL URL - Source URL, visit https://academic.oup.com/clinchem/article/66/8/1012/5880117
 
.pdf PDF - Presentation file, download (1,44 MB)
MD5: 23381B7B5F978EBD283B244E41BF8AD5
 
URL URL - Source URL, visit https://doi.org/10.1093/clinchem/hvaa125
 
Language:English
Typology:1.02 - Review Article
Organization:Logo NIB - National Institute of Biology
Abstract:Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.
Publication status:Published
Publication version:Version of Record
Publication date:03.08.2020
Year of publishing:2020
Number of pages:str. 1012-1029
Numbering:Vol. 66, iss. 8
PID:20.500.12556/DiRROS-20120 New window
UDC:577
ISSN on article:0009-9147
DOI:10.1093/clinchem/hvaa125 New window
COBISS.SI-ID:24618243 New window
Publication date in DiRROS:06.08.2024
Views:513
Downloads:404
Metadata:XML DC-XML DC-RDF
:
HUGGETT, Jim F., WHALE, Alexandra S., SPIEGELAERE, Ward De, ABDEL NOUR, Afif M., BAE, Young-Kyung, BENEŠ, Vladimír, BURKE, Dan, CLEVELAND, Megan, CORBISIER, Philippe, DEVONSHIRE, Alison S., DONG, Lianhua, DRANDI, Daniela, FOY, Carole A., GARSON, Jeremy A., HE, Hua-Jun, HELLEMANS, Jan, KUBISTA, Mikael, LIEVENS, Antoon, MAKRIGIORGOS, Mike G., MILAVEC, Mojca, MUELLER, Reinhold D., NOLAN, Tania, O’SULLIVAN, Denise M., PFAFFL, Michael W., RÖDIGER, Stefan, ROMSOS, Erica L., SHIPLEY, Gregory L., TALY, Valérie, UNTERGASSER, Andreas, WITTWER, Carl T., BUSTIN, Stephen A. and VANDESOMPELE, Jo, 2020, The digital MIQE guidelines update : minimum information for publication of quantitative digital PCR experiments for 2020. Clinical chemistry [online]. 2020. Vol. 66, no. 8, p. 1012–1029. [Accessed 9 April 2025]. DOI 10.1093/clinchem/hvaa125. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=20120
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Record is a part of a journal

Title:Clinical chemistry
Shortened title:Clin Chem
Publisher:P.B. Hoeber
ISSN:0009-9147
COBISS.SI-ID:5865479 New window

Document is financed by a project

Funder:EC - European Commission
Project number:733112
Name:SPIDIA for Personalized Medicine - Standardisation of generic Pre-analytical procedures for In-vitro DIAgnostics for Personalized Medicine
Acronym:SPIDIA4P
Funder:EC - European Commission
Project number:115749
Name:Cancer treatment and monitoring through identification of circulating tumour cells and tumour related nucleic acids in blood
Acronym:CANCER-ID
Funder:Other - Other funder or multiple funders
Funding programme:UK Government Department of Business, Energy and Industrial Strategy, RVO
Project number:86652036
Funder:Other - Other funder or multiple funders
Funding programme:ERDF
Project number:CZ.1.05/1.1.00/02.0109
Acronym:BIOCEV

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:biokemija, digitalna PCR, dPCR, MIQE, dMIQE


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