20.500.12556/DiRROS-20120 The digital MIQE guidelines update minimum information for publication of quantitative digital PCR experiments for 2020 Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology. biokemija digitalna PCR dPCR MIQE dMIQE true false true Angleški jezik Slovenski jezik Neznano 2024-08-06 08:05:58 2024-08-06 08:05:58 2024-08-07 04:07:16 0000-00-00 00:00:00 2020 0 0 str. 1012-1029 iss. 8 Vol. 66 Aug. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-08-03 577 0009-9147 10.1093/clinchem/hvaa125 24618243 https://academic.oup.com/clinchem/article/66/8/1012/5880117 1 3734fdc4-53a9-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28070 RAZ_Huggett_Jim_F._2020.pdf RAZ_Huggett_Jim_F._2020.pdf 1 23381B7B5F978EBD283B244E41BF8AD5 c299b2594d980e843b1a6e600e3260acbffb5982ba8f8eff9337648c8db235c1 d4bcc012-53a9-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28071 https://doi.org/10.1093/clinchem/hvaa125 0 e666f4dc-53a9-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28072 Nacionalni inštitut za biologijo 0 0 0