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Povzetek: Končno poročilo projektne naloge »Dodatne raziskave kvalifikacijskih vrst natura 2000 ter izvajanje spremljanja stanja populacij izbranih ciljnih vrst hroščev v letu 2012« je izvedeno na osnovi pogodbe št. 2330-12-000084, ki je bila sklenjena med Ministrstvo za kmetijstvo in okolje (predstavnik mag. Julijana Lebez Lozej) in Nacionalnim inštitutom za biologijo (predstavnik doc. dr. Al Vrezec). Končno poročilo smo oddali 30.10.2012. Končno poročilo podaja rezultate terenskih vzorčenj v letu 2012. Za močvirskega krešiča (Carabus variolosus), rogača (Lucanus cervus), alpskega kozlička (Rosalia alpina) in bukovega kozlička (Morimus funereus) so podani podatki enoletnega ciklusa monitoringa v letu 2012 po že vzpostavljeni shemi na način kot ga predvideva projektna naloga. Podani so rezultati snemanja populacijskega monitoringa za leto 2012 in dopolnjeni podatki distribucijskega monitoringa v petletnem ciklusu snemanja. Dodatno je za močvirskega krešiča in rogača pripravljena evavlvacija do sedanje sheme monitoringa na podlagi zbranih podatkov v obdobju 2007 – 2012. Za ovratniškega plavača (Graphoderus bilineatus) predvideva naloga dodatne kvalifikacijske raziskave z namenom vzpostavitve sheme monitoringa te vrste v Sloveniji. V tem poročilu so predstavljeni rezultati terenskih raziskav in analize podatkov za leto 2012
Ključne besede: hrošči, monitoring
Objavljeno v DiRROS: 16.09.2024; Ogledov: 653; Prenosov: 618
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Povzetek: V okviru naloge “Dodatne raziskave kvalifikacijskih vrst Natura 2000 ter vzpostavitev in izvajanje monitoringa ciljnih vrst rakov v letu 2010 in 2011 (končno poročilo)”, ki jo je po naročilu Ministrstva RS za okolje in prostor izvajal Center za kartografijo favne in flore s sodelovanjem Nacionalnega inštituta za biologijo in zunanjih sodelavcev (Govedič in sod. 2011) je bil predlagan okvirni načrt monitoringa potočnih rakov v Sloveniji. Večji del raziskav je bil takrat opravljen za namene opredelitve novih Natura 2000 območij za koščaka (Austropotamobius torrentium). Večina predlaganih območij iz leta 2011 (Govedič in sod. 2011) je bila v letu 2013 tudi vključena v razširjeno omrežje Natura 2000 (Uradni list RS 33/2013) in kasneje potrjena na Biogeografskem seminarju leta 2014. V okviru sedanje naloge “Vzpostavitev in izvajanje monitoringa populacij izbranih ciljnih vrst rakov v letih 2014 in 2015” smo izdelali končno metodologijo monitoringa potočnih rakov v Sloveniji, večinoma pa smo sledili predlogu iz leta 2011 (Govedič in sod. 2011). V poročilu so predstavljeni rezultati terenskega dela. Analize populacijskih in distribucijskih trendov še niso mogoče, interpretirali pa smo nekatere izstopajoče rezultate. Glede na prejšnja poročila monitoringa (Govedič in sod. 2007, 2011) smo dopolnili glavne dejavnike ogrožanja potočnih rakov v Sloveniji s predlogi uvedbe nujnega spremljanja v Sloveniji najbolj perečih dejavnikov kot podporo upravljanju območij Natura 2000 za potočne rake.
Ključne besede: raki, razširjenost, Slovenija
Objavljeno v DiRROS: 16.09.2024; Ogledov: 66; Prenosov: 492
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Povzetek: Glioblastoma (GB) is the most common primary malignant brain tumor, characterized by resistance to therapy. Despite aggressive treatment options, GB remains an incurable disease. Invasiveness and heterogeneity are key GB features that cannot be studied in preclinical in vitro models. In this study, we investigated the effects of standard therapy using patient-derived GB organoids (GBOs). GBOs reflect the complexity and heterogeneity of the original tumor tissue. No significant effect on GBO viability or invasion was observed after irradiation and temozolomide treatment. E3 ubiquitin-protein ligase (MDM2), cyclin-dependent kinase inhibitor 1A (CDKN1A), and the serine/threonine kinases ATM and ATR were upregulated at the gene and protein levels after treatment. Our results show that the p53 pathway and DNA-damage response mechanisms were triggered, suggesting that GBOs recapitulate GB therapy resistance. GBOs thus provide a highly efficient platform to assess the specific responses of GB patients to therapy and to further explore therapy resistance.
Ključne besede: cellular physiology, cellular toxicology, in vitro toxicology including 3D culture, technical aspects of cell biology, cancer
Objavljeno v DiRROS: 09.09.2024; Ogledov: 147; Prenosov: 83
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Povzetek: Ulcinj salina is situated in the outermost southern part of Montenegro and covers slightly less than 14.5 km2 of salty basins (total surface area is 1.477 ha). The salina is 1 km from the city of Ulcinj and from the Albanian border. Ulcinj salina is located on the site of the former lagoon and wetland in the delta of the River Bojana. The works on amelioration of the former wetland area started in 1913. That is when the wetland was connected with the sea by the Port Milena channel and isolated itself from the Bojana River with a dike. In 1920 the decision to start with the establishment of the salina in Ulcinj was made. The salina was concluded in 1934 and the first harvesting of salt was done in 1935. The Ulcinj salina was created from the sea and represents a "cultural lagoon" and a man-made landscape. It is surrounded by channels that drain the nearby marshes and “knetas” (adjoining marshlands), not allowing their water to mix with the water from the salina. The channels take the water into the Port Milena canal and then into the sea. The technological process of production was upgraded in the 1970s with construction of the refinery and with attempts to expand the production, both by enlarging the territory of the salina and use of industrial production between 1984 and 1994. Yearly production of salt before the above-mentioned attempts to expand the production (in the period 1935 to 1983) was highly dependent on the weather conditions and organisation of work; a maximum of 41.240 tons of salt was produced in 1952. A combination of manually collected salt and the salt produced through industrial process in the newly constructed factory in the years 1984 to 1994 led to a maximum yearly production of 59.353 tons. By contrast, the production in the last years of production (2003 to 2013) was dramatically lower, reaching on average only around 17.000 tons per year. Ulcinj salina has very hot summers, moderately warm autumns and springs, and mild winters with temperatures only rarely below zero. On average, the most rain falls in autumn and winter, summers are two to three times more dry. Year-on-year and within years, changes in the amount of rain can be significant. Powerful pumps are essential for assuring constant water levels in basins which supports the maintenance of the ecological character of the area. The absence of salt production and consequent changes in water management have caused changes in water salinity, water regime and consequently changes in biodiversity.
Objavljeno v DiRROS: 05.09.2024; Ogledov: 82; Prenosov: 351
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Povzetek: Despite the efforts invested into measures to prevent water eutrophication, like prevention and treatment of point and dispersed discharges, the eutrophication still occurs. One of the unwanted consequences of eutrophic water state is the occurrence of excessive cyanobacterial blooms. Mass occurrence of cyanobacteria is a significant health risk related to surface waters in EU and worldwide since most of the cyanobacterial genera produce cyanotoxins. Besides toxicity, cyanobacterial blooms cause an increase in the turbidity of water and create taste and odour problems. All mentioned represents substantial economic losses in sectors like aquaculture, tourism, drinking water facilities and indirect losses due to increased healthcare expenditures and environmental degradation. It is, therefore, necessary to find appropriate solutions for rapid detection and also in-lake prevention of bloom occurrence despite, for example, existing high eutrophic conditions in the water body. The proposed project represents such a solution. In the frame of the LIFE Stop CyanoBloom project, we have designed two solar powered robotic vessels for the in-lake detection and control of cyanobacterial proliferation. Each vessel is capable of three-dimensional localisation of cyanobacteria by measuring fluorescence of pigments involved in the process of photosynthesis. The vessels are additionally equipped with electrochemical cells using boron doped diamond electrodes fixed on board, producing short-lived hydroxyl radicals, which prevent cyanobacterial proliferation as well as cyanotoxins inactivation. Hydroxyl radicals cause different levels of damages to cyanobacterial cells as well as stress, that may also result in phage induced cyanobacterial lysis. Natural control of cyanobacterial density by lytic cyanophages is known for several years. The mixing of water during the electrolytic cell operation also increases the potential contacts of cyanobacteria with cyanophages, disturbs cyanobacterial buoyancy and generates additional nutrients for nontoxic phytoplankton population. Additional newly developed equipment of the vessel allows advanced automated navigation (automatic docking, avoiding obstacles, maintaining the position in windy conditions, etc.), sampling from different depths, real-time data transfer, statistical analysis and graphical presentation using the adequate software.
Ključne besede: cyanobacteria, cyanobacterial blooms, cyanotoxins, health risk
Objavljeno v DiRROS: 05.09.2024; Ogledov: 90; Prenosov: 668
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