| Naslov: | Integrating metabolic and gene expression profiling of glucosinolate biosynthesis under drought stress in Brassica oleracea |
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| Avtorji: | ID Ben Ammar, Hajer (Avtor)
ID Kabtni, Souhir (Avtor)
ID Arena, Donata (Avtor)
ID Amari, Marwen (Avtor)
ID Al Achkar, Nicolas (Avtor)
ID Branca, Ferdinando (Avtor)
ID Marghali, Sonia (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://www.mdpi.com/1422-0067/27/3/1598
 PDF - Predstavitvena datoteka, prenos (2,15 MB)
MD5: 80EE1A0F102E63586FAAE13CB77595E6
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  KIS - Kmetijski inštitut Slovenije
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| Povzetek: | Drought stress induces pronounced metabolic and transcriptional reprogramming of glucosinolate (GLS) biosynthesis in Brassica oleracea. An integrative approach combining HPLC-based quantification of individual GLSs, quantitative real-time PCR of core biosynthetic and regulatory genes, correlation-based network analysis, and in silico promoter characterization was applied to evaluate drought responses across genetically diverse accessions. Drought triggered strong, accession-specific shifts in GLS composition, with sinigrin content increasing from 35.9% to 55.1% in BR1 and glucoerucin reaching up to 80.2% in CCP1, while indolic GLSs such as glucobrassicin and neoglucobrassicin accounted for >75% of total GLSs in CV2 and CCP3. Hierarchical clustering separated accessions into four distinct drought response clusters independent of morphotype. Correlation analysis revealed drought-induced rewiring of GLS interdependencies, characterized by strengthened positive associations among aliphatic GLSs (r > 0.75). Gene expression profiling identified conserved MYB-centered regulatory modules (MYB28, MYB29, MYB34, MYB122) alongside strong accession-specific induction of CYP79F1 (up to 6.3-fold), FMOGS-OX5 (up to 4.8-fold), and ST5a (up to 5.1-fold). Promoter analysis revealed enrichment of ABA- and stress-responsive cis-regulatory elements. These findings delineate a genotype-dependent regulatory framework underlying GLS plasticity and identify quantitative metabolic and transcriptional markers relevant for breeding drought-resilient Brassica cultivars. |
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| Ključne besede: | glucosinolate, gene expression, qRT-PCR, abiotic stress response, drought stress |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 06.02.2026 |
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| Leto izida: | 2026 |
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| Št. strani: | str. 1-24 |
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| Številčenje: | Vol. 27, iss. 3, ǂarticle no. ǂ1598 |
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| PID: | 20.500.12556/DiRROS-27494  |
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| UDK: | 633 |
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| ISSN pri članku: | 1422-0067 |
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| DOI: | 10.3390/ijms27031598 |
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| COBISS.SI-ID: | 267877635 |
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| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 10. 2. 2026;
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| Datum objave v DiRROS: | 10.02.2026 |
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| Število ogledov: | 389 |
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| Število prenosov: | 78 |
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| Metapodatki: |    |
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