20.500.12556/DiRROS-27494 Integrating metabolic and gene expression profiling of glucosinolate biosynthesis under drought stress in Brassica oleracea Drought stress induces pronounced metabolic and transcriptional reprogramming of glucosinolate (GLS) biosynthesis in Brassica oleracea. An integrative approach combining HPLC-based quantification of individual GLSs, quantitative real-time PCR of core biosynthetic and regulatory genes, correlation-based network analysis, and in silico promoter characterization was applied to evaluate drought responses across genetically diverse accessions. Drought triggered strong, accession-specific shifts in GLS composition, with sinigrin content increasing from 35.9% to 55.1% in BR1 and glucoerucin reaching up to 80.2% in CCP1, while indolic GLSs such as glucobrassicin and neoglucobrassicin accounted for >75% of total GLSs in CV2 and CCP3. Hierarchical clustering separated accessions into four distinct drought response clusters independent of morphotype. Correlation analysis revealed drought-induced rewiring of GLS interdependencies, characterized by strengthened positive associations among aliphatic GLSs (r > 0.75). Gene expression profiling identified conserved MYB-centered regulatory modules (MYB28, MYB29, MYB34, MYB122) alongside strong accession-specific induction of CYP79F1 (up to 6.3-fold), FMOGS-OX5 (up to 4.8-fold), and ST5a (up to 5.1-fold). Promoter analysis revealed enrichment of ABA- and stress-responsive cis-regulatory elements. These findings delineate a genotype-dependent regulatory framework underlying GLS plasticity and identify quantitative metabolic and transcriptional markers relevant for breeding drought-resilient Brassica cultivars. glucosinolate gene expression qRT-PCR abiotic stress response drought stress Brassica oleracea L. true false true Angleški jezik Latinski jezik Neznano 2026-02-10 10:00:18 2026-02-10 10:00:19 2026-02-11 04:01:07 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Opis vira z dne 10. 2. 2026; str. 1-24 iss. 3, ǂarticle no. ǂ1598 Vol. 27 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-02-06 633 1422-0067 10.3390/ijms27031598 267877635 https://www.mdpi.com/1422-0067/27/3/1598 1 eeb29090-065e-11f1-88c7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40247 RAZ_Ben_Ammar_Hajer_2026.pdf RAZ_Ben_Ammar_Hajer_2026.pdf 1 80EE1A0F102E63586FAAE13CB77595E6 e42b6ff28960ec4f5e7c7a6a86cf3f4d2bb903d8fdef2ac57ccbdb442380814d 0f35d72f-065f-11f1-88c7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=40248 Kmetijski inštitut Slovenije 0 0 0