| Naslov: | A rapid method for determination of rosuvastatin in blood plasma with supported liquid extraction |
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| Avtorji: | ID Dermota, Tjaša (Avtor)
ID Božič Mijovski, Mojca (Avtor)
ID Trontelj, Jurij (Avtor) |
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| Datoteke: |  PDF - Predstavitvena datoteka, prenos (2,28 MB)
MD5: E2C709D800C13807E371713873D48E38
 URL - Izvorni URL, za dostop obiščite https://www.sciencedirect.com/science/article/pii/S2667145X25000094
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  UKC LJ - Univerzitetni klinični center Ljubljana
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| Povzetek: | Introduction: Accurate measurement of rosuvastatin in plasma is critical for effective patient management and treatment monitoring following myocardial infarction (MI). Expensive solid-phase extraction (SPE) and timeconsuming liquid-liquid extraction (LLE) have been established for quantifying rosuvastatin. Supported liquid extraction (SLE) could offer a rapid, cost-effective alternative. Objectives: This study aimed to develop and validate a rapid, cost-effective, accurate, and precise method for quantifying rosuvastatin in high-dose plasma samples from patients following MI. Methods: Rosuvastatin was extracted from EDTA plasma using SLE and quantified with LC-MS/MS with positive electrospray ionization. The method was validated according to ICH M10 guidelines, focusing on selectivity, matrix effect, accuracy, precision, linearity, and carryover. Rosuvastatin-D6 was used as an internal standard. Additionally, thirty plasma samples from patients on high-dose rosuvastatin therapy (20 or 40 mg/day) following MI were analyzed by both LLE and SLE methods and compared. Results: The method was successfully validated, demonstrating linearity across a range of 0.1 ng/mL to 50 ng/mL. Compared to the LLE method, SLE achieved superior extraction recovery (96.3 % vs. 60 %) and precision (RSD: 11.9 % vs. 13.6 %) at 0.3 ng/mL rosuvastatin, with a lower absolute matrix effect (12.7 % vs.-36.7 %). Accuracy was comparable (109.3 % vs. 92.8 %). Although SLE involves higher initial costs, it significantly enhances throughput, reduces solvent usage, and minimizes contamination and equipment wear. Conclusion: This study validates SLE as a superior method for quantifying rosuvastatin in plasma, outperforming LLE in recovery, reproducibility, and automation. SLE offers greater accuracy and reliability, making it ideal for high-throughput applications. |
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| Ključne besede: | supported liquid extraction, liquid-liquid extraction, rosuvastatin, LC-MS/MS, myocardial infarction, solid-phase extraction, blood plasma |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Leto izida: | 2025 |
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| Št. strani: | str. 29-36 |
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| Številčenje: | Vol. 36 |
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| PID: | 20.500.12556/DiRROS-24301  |
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| UDK: | 543.544:61 |
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| ISSN pri članku: | 2667-145X |
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| DOI: | 10.1016/j.jmsacl.2025.04.003 |
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| COBISS.SI-ID: | 245998595 |
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| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 20. 8. 2025;
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| Datum objave v DiRROS: | 24.11.2025 |
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| Število ogledov: | 161 |
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| Število prenosov: | 61 |
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| Metapodatki: |    |
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