20.500.12556/DiRROS-24301 A rapid method for determination of rosuvastatin in blood plasma with supported liquid extraction Introduction: Accurate measurement of rosuvastatin in plasma is critical for effective patient management and treatment monitoring following myocardial infarction (MI). Expensive solid-phase extraction (SPE) and timeconsuming liquid-liquid extraction (LLE) have been established for quantifying rosuvastatin. Supported liquid extraction (SLE) could offer a rapid, cost-effective alternative. Objectives: This study aimed to develop and validate a rapid, cost-effective, accurate, and precise method for quantifying rosuvastatin in high-dose plasma samples from patients following MI. Methods: Rosuvastatin was extracted from EDTA plasma using SLE and quantified with LC-MS/MS with positive electrospray ionization. The method was validated according to ICH M10 guidelines, focusing on selectivity, matrix effect, accuracy, precision, linearity, and carryover. Rosuvastatin-D6 was used as an internal standard. Additionally, thirty plasma samples from patients on high-dose rosuvastatin therapy (20 or 40 mg/day) following MI were analyzed by both LLE and SLE methods and compared. Results: The method was successfully validated, demonstrating linearity across a range of 0.1 ng/mL to 50 ng/mL. Compared to the LLE method, SLE achieved superior extraction recovery (96.3 % vs. 60 %) and precision (RSD: 11.9 % vs. 13.6 %) at 0.3 ng/mL rosuvastatin, with a lower absolute matrix effect (12.7 % vs.-36.7 %). Accuracy was comparable (109.3 % vs. 92.8 %). Although SLE involves higher initial costs, it significantly enhances throughput, reduces solvent usage, and minimizes contamination and equipment wear. Conclusion: This study validates SLE as a superior method for quantifying rosuvastatin in plasma, outperforming LLE in recovery, reproducibility, and automation. SLE offers greater accuracy and reliability, making it ideal for high-throughput applications. supported liquid extraction liquid-liquid extraction rosuvastatin LC-MS/MS myocardial infarction solid-phase extraction blood plasma true false true Angleški jezik Ni določen Neznano 2025-11-24 15:32:42 2025-11-24 15:32:42 2025-11-26 03:39:23 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 20. 8. 2025; str. 29-36 Vol. 36 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 543.544:61 2667-145X 10.1016/j.jmsacl.2025.04.003 245998595 RAZ_Dermota_Tjasa_2025.pdf RAZ_Dermota_Tjasa_2025.pdf 1 E2C709D800C13807E371713873D48E38 cf0c7c321532b7e3b81ed721ed369b861b5100f055b6c6438f5439c391cfd21b 999ba696-c942-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36195 https://www.sciencedirect.com/science/article/pii/S2667145X25000094 0 ae679fb3-c942-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36196 Univerzitetni klinični center Ljubljana 0 0 0