| Naslov: | CCL2/C–C chemokine receptor type 2‐mediated interactions among mast cells, basophils, and endothelial cells |
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| Avtorji: | ID Rihar, Maruša (Avtor)
ID Bahri, Rajia (Avtor)
ID Forstnerič, Vida (Avtor)
ID Bulfone‐Paus, Silvia (Avtor)
ID Korošec, Peter (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://onlinelibrary.wiley.com/doi/epdf/10.1002/clt2.70044
 PDF - Predstavitvena datoteka, prenos (1,01 MB)
MD5: 565B8A06A8D5A3AFA586FF5494612E3F
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  KI - Kemijski inštitut
 UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
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| Povzetek: | Background IL-33 is involved in allergic processes by promoting the release of various mast cell (MC) chemokines, including CCL2. However, it is yet unclear which specific cell type is primarily responsible for producing CCL2 during acute allergic reactions. This study aims to investigate the role of IL-33 in promoting CCL2 production in mast cells and assess the effect of MC-derived CCL2 on basophil migration and endothelial permeability. Methods Human blood-derived MCs (hMCs) were generated from peripheral blood precursors, passively sensitized with IgE, treated with IL-33, and stimulated with anti-IgE. The concentrations of nine cytokines known to influence immune cell chemotaxis (CCL2, CCL5, CCL11, MIP-1α, IL-8, IL-10, IL-13, granulocyte-macrophage colony-stimulating factor (GM-CSF), and vascular endothelial growth factor (VEGF) were assessed in the supernatants of hMCs. Subsequently, we investigated the impact of MC-derived CCL2 on basophil migration in vitro, as well as its effect on endothelial monolayer permeability using human umbilical vein endothelial cells (HUVECs). Results Stimulation with anti-IgE induced a significant release of CCL2, GM-CSF, IL-8 and VEGF from hMCs. Additionally, incubation with IL-33 overnight increased the production of several cytokines. Mast cell-derived CCL2 not only enhanced basophil migration in vitro but also increased endothelial monolayer permeability in HUVECs. The effect was reversed by a C–C chemokine receptor type 2 (CCR2) antagonist, indicating the involvement of CCL2 signaling through the CCR2 receptor. Conclusions IL-33 induces the production of chemotactic cytokines in hMCs. Mast cell-derived CCL2 plays an important role in basophil chemotaxis in vitro and affects endothelial monolayer permeability in the HUVEC model. |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 01.02.2025 |
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| Leto izida: | 2025 |
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| Št. strani: | str. 1-9 |
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| Številčenje: | Vol. 15, iss. 2, [article no.] e70019 |
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| Izvor: | Clinical and translational allergy |
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| PID: | 20.500.12556/DiRROS-21774  |
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| UDK: | 616-097 |
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| ISSN pri članku: | 2045-7022 |
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| DOI: | 10.1002/clt2.70044 |
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| COBISS.SI-ID: | 227623171 |
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| Avtorske pravice: | © 2025 The Author(s). Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology. |
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| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 28. 2. 2025;
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| Datum objave v DiRROS: | 27.03.2025 |
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| Število ogledov: | 792 |
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| Število prenosov: | 367 |
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| Metapodatki: |    |
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