20.500.12556/DiRROS-21774 CCL2/C–C chemokine receptor type 2‐mediated interactions among mast cells, basophils, and endothelial cells Background IL-33 is involved in allergic processes by promoting the release of various mast cell (MC) chemokines, including CCL2. However, it is yet unclear which specific cell type is primarily responsible for producing CCL2 during acute allergic reactions. This study aims to investigate the role of IL-33 in promoting CCL2 production in mast cells and assess the effect of MC-derived CCL2 on basophil migration and endothelial permeability. Methods Human blood-derived MCs (hMCs) were generated from peripheral blood precursors, passively sensitized with IgE, treated with IL-33, and stimulated with anti-IgE. The concentrations of nine cytokines known to influence immune cell chemotaxis (CCL2, CCL5, CCL11, MIP-1α, IL-8, IL-10, IL-13, granulocyte-macrophage colony-stimulating factor (GM-CSF), and vascular endothelial growth factor (VEGF) were assessed in the supernatants of hMCs. Subsequently, we investigated the impact of MC-derived CCL2 on basophil migration in vitro, as well as its effect on endothelial monolayer permeability using human umbilical vein endothelial cells (HUVECs). Results Stimulation with anti-IgE induced a significant release of CCL2, GM-CSF, IL-8 and VEGF from hMCs. Additionally, incubation with IL-33 overnight increased the production of several cytokines. Mast cell-derived CCL2 not only enhanced basophil migration in vitro but also increased endothelial monolayer permeability in HUVECs. The effect was reversed by a C–C chemokine receptor type 2 (CCR2) antagonist, indicating the involvement of CCL2 signaling through the CCR2 receptor. Conclusions IL-33 induces the production of chemotactic cytokines in hMCs. Mast cell-derived CCL2 plays an important role in basophil chemotaxis in vitro and affects endothelial monolayer permeability in the HUVEC model. migracija bazofilcev CCL2 prepustnost endotelijske monoplastne plasti človeški mastociti LL-33 basophil migration CCL2 endothelial monolayer permeability human mast cells LL-33 true false true Clinical and translational allergy Angleški jezik Slovenski jezik © 2025 The Author(s). Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology. Neznano 2025-03-27 13:24:38 2025-03-27 13:32:51 2025-10-24 03:49:48 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 28. 2. 2025; str. 1-9 iss. 2, [article no.] e70019 Vol. 15 Feb. 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2025-02-01 616-097 2045-7022 10.1002/clt2.70044 227623171 https://onlinelibrary.wiley.com/doi/epdf/10.1002/clt2.70044 1 74f20853-0b06-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31379 RAZ_Rihar_Marusa_2025.pdf RAZ_Rihar_Marusa_2025.pdf 1 565B8A06A8D5A3AFA586FF5494612E3F 1fc14ff490227125c4e35cd39345e0a78df82e968f7dccc63e05dfa775ee0287 41ad7799-0b07-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31381 Kemijski inštitut Univerzitetna klinika za pljučne bolezni in alergijo Golnik 0 0 0