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Naslov:Methacrylate monolith chromatography as a tool for waterborne virus removal
Avtorji:ID Rački, Nejc (Avtor)
ID Kramberger, Petra (Avtor)
ID Steyer, Andrej (Avtor)
ID Gašperšič, Jernej (Avtor)
ID Štrancar, Aleš (Avtor)
ID Ravnikar, Maja (Avtor)
ID Gutiérrez-Aguirre, Ion (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (1008,58 KB)
MD5: 09BE3FBA25A4338E0A3C020920503F6C
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1016/j.chroma.2015.01.003
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Enteric viruses are commonly present in environmental waters and represent the major cause of waterborne infections and outbreaks. Since traditional wastewater treatments fail to remove enteric viruses in the water purification process, they are released daily into environmental waters. Monolithic supports have enabled chromatography to enter the field of virology. They have been successfully used in virus purification and concentration. In this work quaternary amine (QA) methacrylate monoliths were exploited to remove enteric viruses from wastewater treatment plant effluent. Expectedly, chromatographic processing of such a complex medium was troublesome, even for monoliths, characterized by extremely large pore dimensions. This problem was solved by introducing a pre-step chromatography using hydroxyl (OH) methacrylate monoliths. This way, molecules, that would hinder virus binding to the anion-exchanger monolith, were removed. As a result, the OH pre-column reduced backpressure increase on the subsequent anion-exchanger column, and increased both QA column binding capacity and life time. Wastewater effluent samples were successfully purified from five waterborne enteric viruses (rotavirus, norovirus genogroup I and II, astrovirus, sapovirus), below the detection limit of RT-qPCR. The breakthrough of the rotavirus binding capacity was not reached for concentrations that significantly exceeded those expected in effluent waters. The obtained results confirm that methacrylate monoliths can be a valuable tool for simultaneous removal of different waterborne viruses from contaminated water sources.
Ključne besede:monolith chromatography, waterborne, virus, removal, wastewater, qPCR
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:15.02.2015
Leto izida:2015
Št. strani:str. 118-124
Številčenje:Vol. 1381
PID:20.500.12556/DiRROS-19784 Novo okno
UDK:578
ISSN pri članku:0021-9673
DOI:10.1016/j.chroma.2015.01.003 Novo okno
COBISS.SI-ID:3299151 Novo okno
Datum objave v DiRROS:26.07.2024
Število ogledov:476
Število prenosov:240
Metapodatki:XML DC-XML DC-RDF
:
RAČKI, Nejc, KRAMBERGER, Petra, STEYER, Andrej, GAŠPERŠIČ, Jernej, ŠTRANCAR, Aleš, RAVNIKAR, Maja in GUTIÉRREZ-AGUIRRE, Ion, 2015, Methacrylate monolith chromatography as a tool for waterborne virus removal. Journal of chromatography [na spletu]. 2015. Vol. 1381, p. 118–124. [Dostopano 28 marec 2025]. DOI 10.1016/j.chroma.2015.01.003. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19784
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Gradivo je del revije

Naslov:Journal of chromatography
Skrajšan naslov:J. chromatogr., A
Založnik:Elsevier
ISSN:0021-9673
COBISS.SI-ID:6398471 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:L2-4314-2011
Naslov:Razvoj novih tehnologij za odstranjevanje patogenih mikrobov in toksinov iz različnih vodnih virov
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:34504

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Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
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