20.500.12556/DiRROS-19784 Methacrylate monolith chromatography as a tool for waterborne virus removal Enteric viruses are commonly present in environmental waters and represent the major cause of waterborne infections and outbreaks. Since traditional wastewater treatments fail to remove enteric viruses in the water purification process, they are released daily into environmental waters. Monolithic supports have enabled chromatography to enter the field of virology. They have been successfully used in virus purification and concentration. In this work quaternary amine (QA) methacrylate monoliths were exploited to remove enteric viruses from wastewater treatment plant effluent. Expectedly, chromatographic processing of such a complex medium was troublesome, even for monoliths, characterized by extremely large pore dimensions. This problem was solved by introducing a pre-step chromatography using hydroxyl (OH) methacrylate monoliths. This way, molecules, that would hinder virus binding to the anion-exchanger monolith, were removed. As a result, the OH pre-column reduced backpressure increase on the subsequent anion-exchanger column, and increased both QA column binding capacity and life time. Wastewater effluent samples were successfully purified from five waterborne enteric viruses (rotavirus, norovirus genogroup I and II, astrovirus, sapovirus), below the detection limit of RT-qPCR. The breakthrough of the rotavirus binding capacity was not reached for concentrations that significantly exceeded those expected in effluent waters. The obtained results confirm that methacrylate monoliths can be a valuable tool for simultaneous removal of different waterborne viruses from contaminated water sources. monolith chromatography waterborne virus removal wastewater qPCR true false true Angleški jezik Ni določen Neznano 2024-07-25 04:13:07 2024-07-26 13:29:30 2024-07-27 04:00:54 0000-00-00 00:00:00 2015 0 0 str. 118-124 Vol. 1381 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2015-02-15 578 0021-9673 10.1016/j.chroma.2015.01.003 3299151 RAZ_Racki_Nejc_2015.pdf RAZ_Racki_Nejc_2015.pdf 1 09BE3FBA25A4338E0A3C020920503F6C 444a3f1dedc204529ba812421235f273288c9af1f861acba20cadd571d013180 b6e49ca4-4b31-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27627 https://doi.org/10.1016/j.chroma.2015.01.003 0 d239aa18-4b31-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27629 Nacionalni inštitut za biologijo 0 0 0