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Naslov:RECQ1 helicase silencing decreases the tumour growth rate of U87 glioblastoma cell xenografts in zebrafish embryos
Avtorji:ID Vittori, Miloš (Avtor)
ID Breznik, Barbara (Avtor)
ID Hrovat, Katja (Avtor)
ID Kenig, Saša (Avtor)
ID Lah Turnšek, Tamara (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite http://www.mdpi.com/2073-4425/8/9/222
 
.pdf PDF - Predstavitvena datoteka, prenos (2,73 MB)
MD5: AB39479285BBFF00E71B9A91F4C85613
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:RECQ1 helicase has multiple roles in DNA replication, including restoration of the replication fork and DNA repair, and plays an important role in tumour progression. Its expression is highly elevated in glioblastoma as compared to healthy brain tissue. We studied the effects of small hairpin RNA (shRNA)-induced silencing of RECQ1 helicase on the increase in cell number and the invasion of U87 glioblastoma cells. RECQ1 silencing reduced the rate of increase in the number of U87 cells by 30%. This corresponded with a 40% reduction of the percentage of cells in the G2 phase of the cell cycle, and an accumulation of cells in the G1 phase. These effects were confirmed in vivo, in the brain of zebrafish (Danio rerio) embryos, by implanting DsRed-labelled RECQ1 helicase-silenced and control U87 cells. The growth of resulting tumours was quantified by monitoring the increase in xenograft fluorescence intensity during a three-day period with fluorescence microscopy. The reduced rate of tumour growth, by approximately 30% in RECQ1 helicase-silenced cells, was in line with in vitro measurements of the increase in cell number upon RECQ1 helicase silencing. However, RECQ1 helicase silencing did not affect invasive behaviour of U87 cells in the zebrafish brain. This is the first in vivo confirmation that RECQ1 helicase is a promising molecular target in the treatment of glioblastoma.
Ključne besede:cancer, cell cycle, DNA damage, intravital imaging, RNA interference, theranostics
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:06.09.2017
Leto izida:2017
Št. strani:str. 1-11
Številčenje:Vol. 8, no. 9
PID:20.500.12556/DiRROS-19695 Novo okno
UDK:577.2
ISSN pri članku:2073-4425
DOI:10.3390/genes8090222 Novo okno
COBISS.SI-ID:4411215 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 7. 9. 2017;
Datum objave v DiRROS:25.07.2024
Število ogledov:469
Število prenosov:303
Metapodatki:XML DC-XML DC-RDF
:
VITTORI, Miloš, BREZNIK, Barbara, HROVAT, Katja, KENIG, Saša in LAH TURNŠEK, Tamara, 2017, RECQ1 helicase silencing decreases the tumour growth rate of U87 glioblastoma cell xenografts in zebrafish embryos. Genes [na spletu]. 2017. Vol. 8, no. 9, p. 1–11. [Dostopano 2 april 2025]. DOI 10.3390/genes8090222. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19695
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Gradivo je del revije

Naslov:Genes
Skrajšan naslov:Genes
Založnik:Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:2073-4425
COBISS.SI-ID:523100185 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P1-0245-2015
Naslov:Ekotoksiologija, toksikološka genomika in karcinogeneza
Financer:EC - European Commission
Program financ.:INTERREG project
Številka projekta:42, 2011
Akronim:GLIOMA

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

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