20.500.12556/DiRROS-19695 RECQ1 helicase silencing decreases the tumour growth rate of U87 glioblastoma cell xenografts in zebrafish embryos RECQ1 helicase has multiple roles in DNA replication, including restoration of the replication fork and DNA repair, and plays an important role in tumour progression. Its expression is highly elevated in glioblastoma as compared to healthy brain tissue. We studied the effects of small hairpin RNA (shRNA)-induced silencing of RECQ1 helicase on the increase in cell number and the invasion of U87 glioblastoma cells. RECQ1 silencing reduced the rate of increase in the number of U87 cells by 30%. This corresponded with a 40% reduction of the percentage of cells in the G2 phase of the cell cycle, and an accumulation of cells in the G1 phase. These effects were confirmed in vivo, in the brain of zebrafish (Danio rerio) embryos, by implanting DsRed-labelled RECQ1 helicase-silenced and control U87 cells. The growth of resulting tumours was quantified by monitoring the increase in xenograft fluorescence intensity during a three-day period with fluorescence microscopy. The reduced rate of tumour growth, by approximately 30% in RECQ1 helicase-silenced cells, was in line with in vitro measurements of the increase in cell number upon RECQ1 helicase silencing. However, RECQ1 helicase silencing did not affect invasive behaviour of U87 cells in the zebrafish brain. This is the first in vivo confirmation that RECQ1 helicase is a promising molecular target in the treatment of glioblastoma. cancer cell cycle DNA damage intravital imaging RNA interference theranostics true false true Angleški jezik Ni določen Neznano 2024-07-24 03:45:24 2024-07-25 09:45:26 2024-09-10 13:58:45 0000-00-00 00:00:00 2017 0 0 Nasl. z nasl. zaslona; Opis vira z dne 7. 9. 2017; str. 1-11 no. 9 Vol. 8 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2017-09-06 577.2 2073-4425 10.3390/genes8090222 4411215 http://www.mdpi.com/2073-4425/8/9/222 1 b7f75563-494d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27227 RAZ_Vittori_Milos_2017.pdf RAZ_Vittori_Milos_2017.pdf 1 AB39479285BBFF00E71B9A91F4C85613 4b9e44c35a4379fe2f2ddeb7ad259498cd0418c2f06162c27eb64e359d3c3c7e 22d2b7ae-4a49-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27447 Nacionalni inštitut za biologijo 0 0 0