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Naslov:Spatial and temporal recruitment of the neurovascular unit during development of the mouse blood-retinal barrier
Avtorji:ID Wijk, Anne-Eva van der (Avtor)
ID Vogels, Ilse M.C. (Avtor)
ID Veen, Henk A. van (Avtor)
ID Noorden, Cornelis J. F. van (Avtor)
ID Schlingemann, Reinier O. (Avtor)
ID Klaassen, Ingeborg (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.sciencedirect.com/science/article/pii/S0040816618300600?via%3Dihub
 
.pdf PDF - Predstavitvena datoteka, prenos (8,03 MB)
MD5: AFDA9877D2D21F15A6F57C4F72A3A125
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:The inner blood-retinal barrier (BRB) is made up by the neurovascular unit, consisting of endothelial cells, pericytes and glial cells. The BRB maintains homeostasis of the neural retina, but in pathological eye conditions the neurovascular unit is often disrupted, causing BRB loss. Here, we investigated in detail temporal and spatial recruitment of the neurovascular unit in the neonatal mouse retina from postnatal day (P)3 to P25 employing immunohistochemical staining of vascular endothelium (isolectin B4), pericytes (α-SMA and NG2) and astrocytes (GFAP). In addition, we investigated gene expression of polarized astrocytic end-feet markers aquaporin-4 and laminin α2 chain with qPCR. We observed GFAP-positive cells migrating ahead of the retinal vasculature during the first postnatal week, suggesting that the retinal vasculature follows an astrocytic meshwork. From P9 onwards, astrocytes acquired a mature phenotype, with a more stellate shape and increased expression of aquaporin-4. NG2-positive cells and tip cells co-localized at P5 and invaded the retina together as a vascular sprouting front. In summary, these data suggest that recruitment of the cell types of the neurovascular unit is a prerequisite for proper retinal vascularization and BRB formation.
Ključne besede:endothelial cells, astrocytes, pericytes, retinal development
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.06.2018
Leto izida:2018
Št. strani:str. 42-50
Številčenje:Vol. 52
PID:20.500.12556/DiRROS-19659 Novo okno
UDK:577
ISSN pri članku:0040-8166
DOI:10.1016/j.tice.2018.03.010 Novo okno
COBISS.SI-ID:40481541 Novo okno
Datum objave v DiRROS:24.07.2024
Število ogledov:445
Število prenosov:309
Metapodatki:XML DC-XML DC-RDF
:
WIJK, Anne-Eva van der, VOGELS, Ilse M.C., VEEN, Henk A. van, NOORDEN, Cornelis J. F. van, SCHLINGEMANN, Reinier O. in KLAASSEN, Ingeborg, 2018, Spatial and temporal recruitment of the neurovascular unit during development of the mouse blood-retinal barrier. Tissue & Cell [na spletu]. 2018. Vol. 52, p. 42–50. [Dostopano 2 april 2025]. DOI 10.1016/j.tice.2018.03.010. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19659
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Gradivo je del revije

Naslov:Tissue & Cell
Založnik:Longman Group
COBISS.SI-ID:6101252 Novo okno

Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Dutch Diabetes Fund
Številka projekta:2014.00.1784
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Landelijke Stichting voor Blinden en Slechtzienden, Novartis Fonds and MaculaFonds
Številka projekta:2014-33
Naslov:Grant UitZicht
Akronim:UitZicht
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Nederlandse Vereniging ter Verbetering van het Lot der Blinden, Rotterdamse Stichting Blindenbelangen
Številka projekta:B20140050
Naslov:Grant
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Edmond en Marianne Blaauw Fonds voor Oogheelkunde

Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:biokemija, endotelne celice, astrociti, periciti


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