20.500.12556/DiRROS-19659 Spatial and temporal recruitment of the neurovascular unit during development of the mouse blood-retinal barrier The inner blood-retinal barrier (BRB) is made up by the neurovascular unit, consisting of endothelial cells, pericytes and glial cells. The BRB maintains homeostasis of the neural retina, but in pathological eye conditions the neurovascular unit is often disrupted, causing BRB loss. Here, we investigated in detail temporal and spatial recruitment of the neurovascular unit in the neonatal mouse retina from postnatal day (P)3 to P25 employing immunohistochemical staining of vascular endothelium (isolectin B4), pericytes (α-SMA and NG2) and astrocytes (GFAP). In addition, we investigated gene expression of polarized astrocytic end-feet markers aquaporin-4 and laminin α2 chain with qPCR. We observed GFAP-positive cells migrating ahead of the retinal vasculature during the first postnatal week, suggesting that the retinal vasculature follows an astrocytic meshwork. From P9 onwards, astrocytes acquired a mature phenotype, with a more stellate shape and increased expression of aquaporin-4. NG2-positive cells and tip cells co-localized at P5 and invaded the retina together as a vascular sprouting front. In summary, these data suggest that recruitment of the cell types of the neurovascular unit is a prerequisite for proper retinal vascularization and BRB formation. endothelial cells astrocytes pericytes retinal development biokemija endotelne celice astrociti periciti true false true Angleški jezik Slovenski jezik Neznano 2024-07-23 03:55:28 2024-07-24 14:31:07 2024-07-25 04:14:55 0000-00-00 00:00:00 2018 0 0 str. 42-50 Vol. 52 Jun. 2018 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2018-06-01 577 0040-8166 10.1016/j.tice.2018.03.010 40481541 https://www.sciencedirect.com/science/article/pii/S0040816618300600?via%3Dihub 1 df553df6-4885-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27149 RAZ_Wijk_Anne-Eva_van_der_2018.pdf RAZ_Wijk_Anne-Eva_van_der_2018.pdf 1 AFDA9877D2D21F15A6F57C4F72A3A125 978fa50061705af0f8c5cff6b7dc2426c28d327cc9a38058ef806b0dc71ca69e 0c5a5667-49a8-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27349 Nacionalni inštitut za biologijo 0 0 0