| Naslov: | A small bacteriophage protein determines the hierarchy over co-residential jumbo phage in Bacillus thuringiensis serovar israelensis |
|---|
| Avtorji: | ID Pavlin, Anja (Avtor)
ID Lovše, Anže (Avtor)
ID Bajc, Gregor (Avtor)
ID Otoničar, Jan (Avtor)
ID Kujović, Amela (Avtor)
ID Lengar, Živa (Avtor)
ID Gutiérrez-Aguirre, Ion (Avtor)
ID Kostanjšek, Rok (Avtor)
ID Konc, Janez (Avtor)
ID Fornelos, Nadine (Avtor)
ID Butala, Matej (Avtor) |
|---|
| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://doi.org/10.1038/s42003-022-04238-3
 PDF - Predstavitvena datoteka, prenos (3,46 MB)
MD5: B1EFFAACD1727CA6F20C03CD5E859D44
|
|---|
| Jezik: | Angleški jezik |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: |  NIB - Nacionalni inštitut za biologijo
 KI - Kemijski inštitut
|
|---|
| Povzetek: | Bacillus thuringiensis serovar israelensis is the most widely used biopesticide against insects, including vectors of animal and human diseases. Among several extrachromosomal elements, this endospore-forming entomopathogen harbors two bacteriophages: a linear DNA replicon named GIL01 that does not integrate into the chromosome during lysogeny and a circular-jumbo prophage known as pBtic235. Here, we show that GIL01 hinders the induction of cohabiting prophage pBtic235. The GIL01-encoded small protein, gp7, which interacts with the host LexA repressor, is a global transcription regulator and represses the induction of pBtic235 after DNA damage to presumably allow GIL01 to multiply first. In a complex with host LexA in stressed cells, gp7 down-regulates the expression of more than 250 host and pBtic235 genes, many of which are involved in the cellular functions of genome maintenance, cell-wall transport, and membrane and protein stability. We show that gp7 homologs that are found exclusively in bacteriophages act in a similar fashion to enhance LexA’s binding to DNA, while likely also affecting host gene expression. Our results provide evidence that GIL01 influences both its host and its co-resident bacteriophage. |
|---|
| Status publikacije: | Objavljeno |
|---|
| Verzija publikacije: | Objavljena publikacija |
|---|
| Datum objave: | 24.11.2022 |
|---|
| Leto izida: | 2022 |
|---|
| Št. strani: | str. 1-12 |
|---|
| Številčenje: | [Vol.] 5 |
|---|
| PID: | 20.500.12556/DiRROS-19419  |
|---|
| UDK: | 579.6 |
|---|
| ISSN pri članku: | 2399-3642 |
|---|
| DOI: | 10.1038/s42003-022-04238-3 |
|---|
| COBISS.SI-ID: | 132540419 |
|---|
| Opomba: | Ostali avtorji: Anže Lovše, Gregor Bajc, Jan Otoničar, Amela Kujović, Živa Lengar, Ion Gutierrez-Aguirre, Rok Kostanjšek, Janez Konc, Nadine Fornelos & Matej Butala;
Nasl. z nasl. zaslona;
Opis vira z dne 6. 12. 2022;
|
|---|
| Datum objave v DiRROS: | 18.07.2024 |
|---|
| Število ogledov: | 337 |
|---|
| Število prenosov: | 245 |
|---|
| Metapodatki: |    |
|---|
|
:
|
Kopiraj citat |
|---|
| | | | Objavi na: |  |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |
