| Title: | A small bacteriophage protein determines the hierarchy over co-residential jumbo phage in Bacillus thuringiensis serovar israelensis |
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| Authors: | ID Pavlin, Anja (Author)
ID Lovše, Anže (Author)
ID Bajc, Gregor (Author)
ID Otoničar, Jan (Author)
ID Kujović, Amela (Author)
ID Lengar, Živa (Author)
ID Gutiérrez-Aguirre, Ion (Author)
ID Kostanjšek, Rok (Author)
ID Konc, Janez (Author)
ID Fornelos, Nadine (Author)
ID Butala, Matej (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.1038/s42003-022-04238-3
 PDF - Presentation file, download (3,46 MB)
MD5: B1EFFAACD1727CA6F20C03CD5E859D44
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
 KI - National Institute of Chemistry
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| Abstract: | Bacillus thuringiensis serovar israelensis is the most widely used biopesticide against insects, including vectors of animal and human diseases. Among several extrachromosomal elements, this endospore-forming entomopathogen harbors two bacteriophages: a linear DNA replicon named GIL01 that does not integrate into the chromosome during lysogeny and a circular-jumbo prophage known as pBtic235. Here, we show that GIL01 hinders the induction of cohabiting prophage pBtic235. The GIL01-encoded small protein, gp7, which interacts with the host LexA repressor, is a global transcription regulator and represses the induction of pBtic235 after DNA damage to presumably allow GIL01 to multiply first. In a complex with host LexA in stressed cells, gp7 down-regulates the expression of more than 250 host and pBtic235 genes, many of which are involved in the cellular functions of genome maintenance, cell-wall transport, and membrane and protein stability. We show that gp7 homologs that are found exclusively in bacteriophages act in a similar fashion to enhance LexA’s binding to DNA, while likely also affecting host gene expression. Our results provide evidence that GIL01 influences both its host and its co-resident bacteriophage. |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 24.11.2022 |
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| Year of publishing: | 2022 |
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| Number of pages: | str. 1-12 |
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| Numbering: | [Vol.] 5 |
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| PID: | 20.500.12556/DiRROS-19419  |
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| UDC: | 579.6 |
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| ISSN on article: | 2399-3642 |
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| DOI: | 10.1038/s42003-022-04238-3 |
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| COBISS.SI-ID: | 132540419 |
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| Note: | Ostali avtorji: Anže Lovše, Gregor Bajc, Jan Otoničar, Amela Kujović, Živa Lengar, Ion Gutierrez-Aguirre, Rok Kostanjšek, Janez Konc, Nadine Fornelos & Matej Butala;
Nasl. z nasl. zaslona;
Opis vira z dne 6. 12. 2022;
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| Publication date in DiRROS: | 18.07.2024 |
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| Views: | 342 |
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| Downloads: | 248 |
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