| Naslov: | Digital PCR for the characterization of reference materials |
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| Avtorji: | ID Cleveland, Megan H. (Avtor)
ID He, Hua-Jun (Avtor)
ID Milavec, Mojca (Avtor)
ID Bae, Young-Kyung (Avtor)
ID Vallone, Peter M. (Avtor)
ID Huggett, Jim F. (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://www.sciencedirect.com/science/article/pii/S0098299724000153?via%3Dihub
 PDF - Predstavitvena datoteka. (773,16 KB, Vsebina dokumenta nedostopna do 14.02.2025)
MD5: CE80FD876F64302FE3D7BF3F699C72A6
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.02 - Pregledni znanstveni članek |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials. |
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| Ključne besede: | digital PCR, dPCR, reference materials, UV-spectroscopy |
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| Status publikacije: | Posredovano založniku |
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| Verzija publikacije: | Recenzirani rokopis |
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| Datum objave: | 14.02.2024 |
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| Leto izida: | 2024 |
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| Št. strani: | str. 1-8 |
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| Številčenje: | Vol. 96, [article no.] 101256 |
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| PID: | 20.500.12556/DiRROS-19040  |
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| UDK: | 577.2 |
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| ISSN pri članku: | 1872-9452 |
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| DOI: | 10.1016/j.mam.2024.101256 |
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| COBISS.SI-ID: | 186484995 |
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| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 22. 2. 2024;
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| Datum objave v DiRROS: | 03.06.2024 |
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| Število ogledov: | 538 |
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| Število prenosov: | 193 |
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| Metapodatki: |    |
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