20.500.12556/DiRROS-19040 Digital PCR for the characterization of reference materials Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials. digital PCR dPCR reference materials UV-spectroscopy digitalna PCR digitalna verižna reakcija s polimerazo referenčni materiali UV-spektroskopija true false true Angleški jezik Slovenski jezik Neznano 2024-06-03 16:24:25 2024-06-03 16:24:25 2024-06-04 03:38:30 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 22. 2. 2024; str. 1-8 [article no.] 101256 Vol. 96 2024 0000-00-00 PostprintKoncna PosredovanoVObjavo apc 0000-00-00 0000-00-00 2024-02-14 2025-02-14 577.2 1872-9452 10.1016/j.mam.2024.101256 186484995 https://www.sciencedirect.com/science/article/pii/S0098299724000153?via%3Dihub 1 fb8c5d0d-21b4-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26138 RAZ_Cleveland_Megan_H._2024.pdf RAZ_Cleveland_Megan_H._2024.pdf 1 CE80FD876F64302FE3D7BF3F699C72A6 f3e6ae89313ec709a86f82650c34b87b653e4f83857126140f3aa5c903bf17ad 64a5b28b-21b5-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26139 Nacionalni inštitut za biologijo 0 0 0