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Naslov:Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathon
Avtorji:Jan, Zala (Avtor)
Drab, Mitja (Avtor)
Drobne, Damjana (Avtor)
Bedina Zavec, Apolonija (Avtor)
Benčina, Mojca (Avtor)
Drašler, Barbara (Avtor)
Hočevar, Matej (Avtor)
Krek, Judita Lea (Avtor)
Pađen, Ljubiša (Avtor)
Pajnič, Manca (Avtor)
Repar, Neža (Avtor)
Šimunič, Boštjan (Avtor)
Štukelj, Roman (Avtor)
Kralj-Iglič, Veronika (Avtor)
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo ZRS Koper - Znanstveno-raziskovalno središče Koper / Centro di Ricerche Scientifiche Capodistria
Povzetek:Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
Ključne besede:membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon
Leto izida:2021
UDK:577.1:796.422.16
ISSN pri članku:1178-2013
OceCobissID:3223828 Povezava se odpre v novem oknu
COBISS_ID:48113411 Povezava se odpre v novem oknu
DOI:10.2147/IJN.S282200 Povezava se odpre v novem oknu
Opombe:Nasl. z nasl. zaslona; Opis vira z dne 21. 1. 2021; Soavtorji: Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drasler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič;
Število ogledov:706
Število prenosov:486
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (1,88 MB)
URL URL - Izvorni URL, za dostop obiščite https://www.dovepress.com/decrease-in-cellular-nanovesicles-concentration-in-blood-of-athletes-m-peer-reviewed-article-IJN
 
Nadgradivo:International journal of nanomedicine
Dove Medical Press
 
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Avtorske pravice:© 2021 Jan et al.
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Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:20.01.2021

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