Title: | Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathon |
---|
Authors: | ID Jan, Zala (Author) ID Drab, Mitja (Author) ID Drobne, Damjana (Author) ID Bedina Zavec, Apolonija (Author) ID Benčina, Mojca (Author) ID Drašler, Barbara (Author) ID Hočevar, Matej (Author) ID Krek, Judita Lea (Author) ID Pađen, Ljubiša (Author) ID Pajnič, Manca (Author) ID Repar, Neža (Author) ID Šimunič, Boštjan (Author) ID Štukelj, Roman (Author) ID Kralj-Iglič, Veronika (Author) |
Files: | PDF - Presentation file, download (1,88 MB) MD5: 15BA5FE84BDD421A0549244AB94233B7
URL - Source URL, visit https://doi.org/10.2147/IJN.S282200
URL - Source URL, visit https://www.dovepress.com/decrease-in-cellular-nanovesicles-concentration-in-blood-of-athletes-m-peer-reviewed-article-IJN
|
---|
Language: | English |
---|
Typology: | 1.01 - Original Scientific Article |
---|
Organization: | ZRS Koper - Science and Research Centre Koper
|
---|
Abstract: | Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds. |
---|
Keywords: | membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon |
---|
Publication status: | Published |
---|
Publication version: | Version of Record |
---|
Year of publishing: | 2021 |
---|
Number of pages: | str. 443-456 |
---|
Numbering: | Vol. 16 |
---|
PID: | 20.500.12556/DiRROS-13691 |
---|
UDC: | 577.1:796.422.16 |
---|
ISSN on article: | 1178-2013 |
---|
DOI: | 10.2147/IJN.S282200 |
---|
COBISS.SI-ID: | 48113411 |
---|
Copyright: | © 2021 Jan et al. |
---|
Note: | Nasl. z nasl. zaslona;
Opis vira z dne 21. 1. 2021;
Soavtorji: Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drasler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič;
|
---|
Publication date in DiRROS: | 25.01.2021 |
---|
Views: | 1269 |
---|
Downloads: | 1574 |
---|
Metadata: | |
---|
:
|
Copy citation |
---|
| | | Share: | |
---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |