Digitalni repozitorij raziskovalnih organizacij Slovenije

Izpis gradiva
A+ | A- | SLO | ENG

Naslov:Next-generation sequencing of drug resistant Mycobacterium tuberculosis clinical isolates in low-incidence countries
Avtorji:Sodja, Eva (Avtor)
Toplak, Nataša (Avtor)
Koren, Simon (Avtor)
Kovač, Minka (Avtor)
Truden, Sara (Avtor)
Ilievska Poposka, Biljana (Avtor)
Žolnir-Dovč, Marija (Avtor)
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
Povzetek:Drug resistant tuberculosis (TB), especially multidrug (MDR) and extensively drug-resistant (XDR) TB, is still a serious problem in global TB control. Slovenia and North Macedonia are low-incidence countries with TB incidence rates of 5.4 and 10.4 in 2017, respectively. In both countries, the percentage of drug resistant TB is very low with sporadic cases of MDR-TB. However, global burden of drug-resistant TB continues to increase imposing huge impact on public health systems and strongly stimulating the detection of gene variants related with drug resistance in TB. Next-generation sequencing (NGS) can provide comprehensive analysis of gene variants linked to drug resistance in Mycobacterium tuberculosis. Therefore, the aim of our study was to examine the feasibility of a full-length gene analysis for the drug resistance related genes (inhA, katG, rpoB, embB) using Ion Torrent technology and to compare the NGS results with those obtained from conventional phenotypic drug susceptibility testing (DST) in TB isolates. Between 1996 and 2017, we retrospectively selected 56 TB strains from our National mycobacterial culture collection. Of those, 33 TB isolates from Slovenian patients were isolated from various clinical samples and subjected to phenotypic DST testing in Laboratory for Mycobacteria (University Clinic Golnik, Slovenia). The remaining 23 TB isolates were isolated from Macedonian patients and sent to our laboratory for assistance in phenotypic DST testing. TB strains included were either mono-, poly- or multidrug resistant. For control purposes, we also randomly selected five TB strains susceptible to first-line anti-TB drugs. High concordance between genetic (Ion Torrent technology) and standard phenotypic DST testing for isoniazid, rifampicin and ethambutol was observed, with percent of agreement of 77%, 93.4% and 93.3%, sensitivities of 68.2%, 100% and 100%, and specificities of 100%, 80% and 88.2%, respectively. In conclusion, the genotypic DST using Ion Torrent semiconductor NGS successfully predicted drug resistance with significant shortening of time needed to obtain the resistance profiles from several weeks to just a few days.
Ključne besede:drug resistant tuberculosis, next-generation sequencing, low-incidence countries, phenotypic drug susceptibility testing
Leto izida:2019
Založnik:Saint-Petersburg Pasteur Institute
Izvor:Rusija
UDK:616-002
ISSN pri članku:2313-7398
OceCobissID:525094169 Povezava se odpre v novem oknu
COBISS_ID:13128195 Povezava se odpre v novem oknu
DOI:10.15789/2220-7619-2019-5-6-773-778 Povezava se odpre v novem oknu
Opombe:Nasl. z nasl. zaslona; Soavtorji iz Slovenije: N. Toplak, S. Koren, M. Kovač, S. Truden, M. Žolnir-Dovč; Opis vira z dne 29. 4. 2020;
Število ogledov:1018
Število prenosov:464
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (144,02 KB)
URL URL - Izvorni URL, za dostop obiščite https://www.iimmun.ru/iimm/article/view/851/883
 
Nadgradivo:Infekc. immun.
NIIÈM imeni Pastera
 
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Avtorske pravice:Sodja E. et al., 2019
:
  
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše podrobnosti ali sproži prenos.


Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:24.07.2020

Nazaj