Digitalni repozitorij raziskovalnih organizacij Slovenije

Izpis gradiva
A+ | A- | SLO | ENG

Naslov:Next-generation sequencing of drug resistant Mycobacterium tuberculosis clinical isolates in low-incidence countries
Avtorji:ID Sodja, Eva, Klinika Golnik (Avtor)
ID Toplak, Nataša (Avtor)
ID Koren, Simon (Avtor)
ID Kovač, Minka (Avtor)
ID Truden, Sara, Klinika Golnik (Avtor)
ID Ilievska Poposka, Biljana (Avtor)
ID Žolnir-Dovč, Marija, Klinika Golnik (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (144,02 KB)
MD5: C2CFCA57A3FFF065BB70F7D7CFD360E8
 
URL URL - Izvorni URL, za dostop obiščite https://www.iimmun.ru/iimm/article/view/851/883
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
Povzetek:Drug resistant tuberculosis (TB), especially multidrug (MDR) and extensively drug-resistant (XDR) TB, is still a serious problem in global TB control. Slovenia and North Macedonia are low-incidence countries with TB incidence rates of 5.4 and 10.4 in 2017, respectively. In both countries, the percentage of drug resistant TB is very low with sporadic cases of MDR-TB. However, global burden of drug-resistant TB continues to increase imposing huge impact on public health systems and strongly stimulating the detection of gene variants related with drug resistance in TB. Next-generation sequencing (NGS) can provide comprehensive analysis of gene variants linked to drug resistance in Mycobacterium tuberculosis. Therefore, the aim of our study was to examine the feasibility of a full-length gene analysis for the drug resistance related genes (inhA, katG, rpoB, embB) using Ion Torrent technology and to compare the NGS results with those obtained from conventional phenotypic drug susceptibility testing (DST) in TB isolates. Between 1996 and 2017, we retrospectively selected 56 TB strains from our National mycobacterial culture collection. Of those, 33 TB isolates from Slovenian patients were isolated from various clinical samples and subjected to phenotypic DST testing in Laboratory for Mycobacteria (University Clinic Golnik, Slovenia). The remaining 23 TB isolates were isolated from Macedonian patients and sent to our laboratory for assistance in phenotypic DST testing. TB strains included were either mono-, poly- or multidrug resistant. For control purposes, we also randomly selected five TB strains susceptible to first-line anti-TB drugs. High concordance between genetic (Ion Torrent technology) and standard phenotypic DST testing for isoniazid, rifampicin and ethambutol was observed, with percent of agreement of 77%, 93.4% and 93.3%, sensitivities of 68.2%, 100% and 100%, and specificities of 100%, 80% and 88.2%, respectively. In conclusion, the genotypic DST using Ion Torrent semiconductor NGS successfully predicted drug resistance with significant shortening of time needed to obtain the resistance profiles from several weeks to just a few days.
Ključne besede:drug resistant tuberculosis, next-generation sequencing, low-incidence countries, phenotypic drug susceptibility testing
Leto izida:2019
Status objave pri reviji:Objavljeno
Verzija članka:Založnikova različica članka
Založnik:Saint-Petersburg Pasteur Institute
Št. strani:str. 773-778
Številčenje:Vol. 9, no. 5/6
Izvor:Rusija
UDK:616-002
ISSN pri članku:2313-7398
COBISS.SI-ID:13128195 Novo okno
DOI:10.15789/2220-7619-2019-5-6-773-778 Novo okno
Avtorske pravice:Sodja E. et al., 2019
Opomba:Nasl. z nasl. zaslona; Soavtorji iz Slovenije: N. Toplak, S. Koren, M. Kovač, S. Truden, M. Žolnir-Dovč; Opis vira z dne 29. 4. 2020;
Datum objave v DiRROS:24.07.2020
Število ogledov:1235
Število prenosov:615
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
:
  
Objavi na:AddThis
AddThis uporablja piškotke, za katere potrebujemo vaše privoljenje.
Uredi privoljenje...

Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše podrobnosti ali sproži prenos.

Gradivo je del revije

Naslov:Infekciâ i immunitet
Skrajšan naslov:Infekc. immun.
Založnik:NIIÈM imeni Pastera
ISSN:2313-7398
COBISS.SI-ID:525094169 Novo okno

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:24.07.2020

Sekundarni jezik

Jezik:Ni določen
Ključne besede:proti zdravilom odporna tuberkuloza, sekvenciranje naslednje generacije, države z nizko incidenco, fenotipsko testiranje občutljivosti za zdravila


Nazaj