20.500.12556/DiRROS-12195 Next-generation sequencing of drug resistant Mycobacterium tuberculosis clinical isolates in low-incidence countries Drug resistant tuberculosis (TB), especially multidrug (MDR) and extensively drug-resistant (XDR) TB, is still a serious problem in global TB control. Slovenia and North Macedonia are low-incidence countries with TB incidence rates of 5.4 and 10.4 in 2017, respectively. In both countries, the percentage of drug resistant TB is very low with sporadic cases of MDR-TB. However, global burden of drug-resistant TB continues to increase imposing huge impact on public health systems and strongly stimulating the detection of gene variants related with drug resistance in TB. Next-generation sequencing (NGS) can provide comprehensive analysis of gene variants linked to drug resistance in Mycobacterium tuberculosis. Therefore, the aim of our study was to examine the feasibility of a full-length gene analysis for the drug resistance related genes (inhA, katG, rpoB, embB) using Ion Torrent technology and to compare the NGS results with those obtained from conventional phenotypic drug susceptibility testing (DST) in TB isolates. Between 1996 and 2017, we retrospectively selected 56 TB strains from our National mycobacterial culture collection. Of those, 33 TB isolates from Slovenian patients were isolated from various clinical samples and subjected to phenotypic DST testing in Laboratory for Mycobacteria (University Clinic Golnik, Slovenia). The remaining 23 TB isolates were isolated from Macedonian patients and sent to our laboratory for assistance in phenotypic DST testing. TB strains included were either mono-, poly- or multidrug resistant. For control purposes, we also randomly selected five TB strains susceptible to first-line anti-TB drugs. High concordance between genetic (Ion Torrent technology) and standard phenotypic DST testing for isoniazid, rifampicin and ethambutol was observed, with percent of agreement of 77%, 93.4% and 93.3%, sensitivities of 68.2%, 100% and 100%, and specificities of 100%, 80% and 88.2%, respectively. In conclusion, the genotypic DST using Ion Torrent semiconductor NGS successfully predicted drug resistance with significant shortening of time needed to obtain the resistance profiles from several weeks to just a few days. drug resistant tuberculosis next-generation sequencing low-incidence countries phenotypic drug susceptibility testing proti zdravilom odporna tuberkuloza sekvenciranje naslednje generacije države z nizko incidenco fenotipsko testiranje občutljivosti za zdravila true false true Saint-Petersburg Pasteur Institute Angleški jezik Ni določen Slovenia; Republic of North Macedonia; Slovenija; Republika Severna Makedonija Sodja E. et al., 2019 Neznano 2020-07-24 13:16:15 2020-07-24 13:16:15 2022-08-18 03:35:46 0000-00-00 00:00:00 2019 0 Rusija 0 Nasl. z nasl. zaslona; Soavtorji iz Slovenije: N. Toplak, S. Koren, M. Kovač, S. Truden, M. Žolnir-Dovč; Opis vira z dne 29. 4. 2020; str. 773-778 no. 5/6 Vol. 9 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-002 2313-7398 10.15789/2220-7619-2019-5-6-773-778 13128195 525094169 RAZ_Sodja_Eva_i2019.pdf RAZ_Sodja_Eva_i2019.pdf 1 C2CFCA57A3FFF065BB70F7D7CFD360E8 7a4f708899de9c16859512f3765624fc195cee49980adc352a6ded2244d8eba4 8f7368ad-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=17336 https://www.iimmun.ru/iimm/article/view/851/883 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=14609 Univerzitetna klinika za pljučne bolezni in alergijo Golnik 0 0 0