51. Comparison of colorimetric MTT and clonogenic assays for irradiation and cisplatin treatment on murine fibrosarcoma SA-1 cellsMaja Čemažar, Darja Marolt, Mira Lavrič, Gregor Serša, 1999, izvirni znanstveni članek Povzetek: Background. The aim of our study was to determine the relationship between cell survival of SA-1 tumor cells measured by clonogenic assay and MTT assay after irradiation and cisplatin treatment. Materials and methods. Survival of SA-1 cells was measured after irradiation (2-8 Gy) and cisplatin treatment (0.05-0.5 u g/ml) by clonogenic assay performed 7 days after treatment, and byMTT assay performed on day 3, 4, 5, and 7 after the treatment. Results. The results showed good correlation between MTT assay and clonogenic assay for irradiation doses below 4 Gy. For higher doses good correlation between MTT and clonogenic assay was determined only when MTT assay was performed on day 5and 7 after the treatment. In the case of cisplatin treatment, similar pattern was observed, good correlation between IC50 values for MTT and clonogenic assay was found when MTT assay was performed on day 5 and 7 after the treatment. Conclusion. Results of our study confirmed the results of previous studies addressing this topic and further support the use of MTT testas an alternative test for clonogenic test as a predictive assay of tumourresponse to the radio or chemotherapy. Objavljeno v DiRROS: 22.01.2024; Ogledov: 170; Prenosov: 35 Celotno besedilo (419,17 KB) |
52. Adenocarcinoma skin metastases treated by electrochemotherapy with cisplatin combined with radiationGregor Serša, Maja Čemažar, Zvonimir Rudolf, Albert-Peter Fras, 1999, izvirni znanstveni članek Povzetek: Background. The aim of this study was to determine the interaction between electrochemotherapy as a means of facilitated cisplatin delivery into the cells and irradiation of adenocarcinoma skin metastases. Case report. A patient with progressive disease presenting skin metastases of tubal dedifferentiated pappilary adenocarcinoma was enrolled in the study. Skin metastases were treated by electrochemotherapy with intratumoral injection of cisplatin. Its antitumor effectiveness was compared to that of combined treatment of irradiation with electrochemotherapy. After a two week observation time, the response to treatment was comparable between the electrochemotherapy and electrochemotherapy combined with irradiation. Both ofthese treatments were more effective than irradiation alone. Furthermore, antitumor effectiveness of the combined electrochemo- and radiotherapy was found to be quicker than that of electrochemotherapy alone. Comment. This study shows that electrochemotherapy with cisplatin is also effective in the treatment of adenocarcinoma skin metastases. Inspite of the short observation time, positive interaction between radiotherapy and electrochemotherapy with cisplatin was found. Objavljeno v DiRROS: 22.01.2024; Ogledov: 153; Prenosov: 37 Celotno besedilo (410,27 KB) |
53. Cryosurgery combined with radiotherapy of tumors in miceAlbert-Peter Fras, Simona Kranjc Brezar, Maja Čemažar, Gregor Serša, 1999, izvirni znanstveni članek Povzetek: Tlle aim of this study zuas to determine antitumor effectiveness of cryosurgery alone and in comhination with radiotherapy. Cryosurgery of subcutaneous fibrosarcoma SA-1 tumors in A/J mice was moderately effective trcatmcnt. Tumor growth delay was 10.3 * 3.8 days after 5 minute treatment with nitrogen filled cryo-prohe. Shorter treatment times induced less, but dose dependent antitumor effect. In combined treat- ment, tumors were either first treated by cryosurgery for 3 minutes and then locally irradiated with 10Gy for 5 minutes, or irradiated first and thereafter treated by cryosurgery.The antitumor effectiveness of com- bined treatrnent was sequence dependent; the irradiation of tumors before cryosurgery resulted in better antitumor effect than the irradiation after cryosurgery. These results indicate that radiosensitization may not be always expected, in spite of some reports demonstrating that cryosurgery may have radiosensitizing effect in vivo, and that some other mechanisms may be involved contributing to radiationdamage when cryosurgery follows irradiation. Objavljeno v DiRROS: 22.01.2024; Ogledov: 164; Prenosov: 42 Celotno besedilo (311,91 KB) |
54. Predictive assays of tumor response to chemo and radiotherapyMaja Čemažar, 1999, pregledni znanstveni članek Povzetek: Predstavljeni so testi za napovedovanje izida zdravljenja tumorjev po kemo- inradioterapiji in poškodb normalnih tkiv po radioterapiji. Pregled je osredotočen na tiste teste, pri katerih je treba celice gojiti v pogojih in vitro. Poleg tega članek obravnava tudi korelacijo med napovednimi testi in izidom zdravljenja različnih vrst raka ter poškodbami normalnih tkiv. Objavljeno v DiRROS: 22.01.2024; Ogledov: 149; Prenosov: 44 Celotno besedilo (692,06 KB) |
55. Molecular alterations induced in drug-resistant cellsMaja Osmak, 1998, izvirni znanstveni članek Povzetek: The major obstacle to the ultimate success in cancer therapy is the ability oftumor cells to develop resistance to anticancer drugs. Several molecular mechanisms have been suggested to be involved in drugresistance: a) decrease in the intracellular drug accumulation (increased activity of membrane transporters such as P-glycoprotein or multidrug-resistance-associated protein), b) changes in intracellular detoxification system (increased concentrations of glutathione or metallothioneins, or increased activity of related enzymed), c) alteration in nuclear enzymes (enhanced DNA repair and/orbetter tolerance of DNA damage, decreased acitivty of topoisomerases), d) altered expression of oncogenes (inducing increased level of protective molecules in cells or the inhibition of apoptosis). Drug resistance is a multifactorial phenomenon. The complexity of molecular alterations in drug-resistant cells is and will stay the main problem for the successful treatment of cancer. Objavljeno v DiRROS: 19.01.2024; Ogledov: 143; Prenosov: 26 Celotno besedilo (2,17 MB) |
56. Znanje, ki pušča sled : sedemdeset let Zavoda za gradbeništvo Slovenije 1949-20192019, strokovna monografija Povzetek: V publikaciji je predstavljen Zavod za gradbeništvo Slovenije, ob 70-letnici. V osmih poglavjih je predstavljeno znanstveno-raziskovalno in strokovno delo. V nadaljevanju sledijo poglavja, ki predstavljajo posamezne zanszveno-raziskovalne laboratorije in strokovne službe. Ključne besede: Zavod za gradbeništvo Slovenije, ZAG Ljubljana, gradbeništvo, znanstveno-raziskovalna dejavnost, strokovna dejavnost, laboratoriji, predstavitev, 70 let, sedemdeset let, grajeno okolje, digitalizacija, gradnja, les, krožno gospodarstvo, napredni materiali v gradbeništvu, potresi, stavbe, trajnostna gradnja, bivanje, ekologija, voda, zrak, zemlja Objavljeno v DiRROS: 19.01.2024; Ogledov: 191; Prenosov: 150 Celotno besedilo (19,25 MB) Gradivo ima več datotek! Več... |
57. Knowledge that leaves a mark : seventy years of the Slovenian National Building and Civil Engineering Institute 1949-20192019, strokovna monografija Povzetek: V publikaciji je predstavljen Zavod za gradbeništvo Slovenije ob 70-letnici. V osmih poglavjih je predstavljeno znanstveno-raziskovalno in strokovno delo. V nadaljevanju sledijo poglavja, ki predstavljajo posamezne znanstveno-raziskovalne laboratorije in strokovne službe. Ključne besede: Zavod za gradbeništvo Slovenije, ZAG Ljubljana, gradbeništvo, znanstveno-raziskovalna dejavnost, strokovna dejavnost, laboratoriji, predstavitev, 70 let, sedemdeset let, grajeno okolje, digitalizacija, gradnja, les, krožno gospodarstvo, napredni materiali v gradbeništvu, potresi, stavbe, trajnostna gradnja, bivanje, ekologija, voda, zrak, zemlja Objavljeno v DiRROS: 19.01.2024; Ogledov: 177; Prenosov: 119 Celotno besedilo (19,17 MB) Gradivo ima več datotek! Več... |
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59. Vinblastine increases antitumor effectiveness of bleomycinMaja Čemažar, Marija Auersperg, Gregor Serša, 1997, izvirni znanstveni članek Povzetek: In our previous vinblastine (VELBE) was shown to increase the plasma membrane fluidity. This effect of VELBE might be expolited for better transport of drugs through the plasma membrane. Bleomycin (BLM) is a highly cytotoxic drug when present inside the cells but has a hampered transport through the plasma membrane. The aim of the present study was to determine whether pretreatment with VELBE can increase the effect of BLM on intraperitoneal SA-1 tumors in mice. BLM and VELBE were used as single agents or in various combinations, i.e. VELBE and BLM injected simultanously, BLM injected 24 h before VELBE or VELBE injected 24 h before BLM. Mice survival was the end-point used for determining the effect of this combined treatments. VELBE and BLM as a single treatment significantly prolonged median survival time of study animals compared to controls. Furthermore, when VELBE and BLM were combined, all threetested combinations were more effective than VELBE or BLM as single treatments. The effect on animal survival was equal when VELBE was given 24 h after or simultanously with BLM. The longest survival, however was obtained when VELBE was injected 24 h before BLM. From these results we can conclude that the underlying mechanisms for more than additive effect of VELBE and BLM when VELBE was given 24 h before BLM could be attributed to an increased membrane fluidity, possibly in combination with a cell kinetic effect. Objavljeno v DiRROS: 18.01.2024; Ogledov: 141; Prenosov: 31 Celotno besedilo (1,28 MB) |
60. Study of blood perfusion with patent blue staining method in LPB fibrosarcoma tumors in immuno-competent and immuno-deficient mice after electrotherapy by direct currentTomaž Jarm, Dong Jian An, Jean Belehradek, Lluis M. Mir, Gregor Serša, Maja Čemažar, Tadej Kotnik, Jani Pušenjak, Damijan Miklavčič, 1997, izvirni znanstveni članek Objavljeno v DiRROS: 16.01.2024; Ogledov: 151; Prenosov: 34 Celotno besedilo (344,22 KB) |