1. Protitumorsko delovanje utišanja transkripcijskega faktorja E2F1 na modelu humanega kolorektalnega karcinoma : in vitro in in vivo spoznanjaSimona Kranjc Brezar, Tanja Jesenko, Monica Cazzato, Živa Pišljar, Boštjan Markelc, Tim Božič, Gabriele Grassi, Maja Čemažar, 2024, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje) Ključne besede: rak debelega črevesa in danke, eksperimentalna onkologija, onkologija
Objavljeno v DiRROS: 25.02.2026; Ogledov: 116; Prenosov: 19
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2. Validacija nanoteles za vezavo na specifične antigene mukoznega melanoma (MM)Urša Lampreht Tratar, Ario De Marco, Urša Štrancar, Tim Božič, Barbara Perić, Maja Čemažar, 2024, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: melanom, eksperimentalna onkologija, onkologija
Objavljeno v DiRROS: 25.02.2026; Ogledov: 87; Prenosov: 20
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4. Chemokine CCL5 overexpression combined with radiotherapy modulates Th1-mediated immune response and leads to significant tumor growth delay in mouse tumor modelsTim Božič, Iva Šantek, Živa Pišljar, Simona Kranjc Brezar, Gregor Serša, Boštjan Markelc, Maja Čemažar, 2026, izvirni znanstveni članek Povzetek: This study investigated the antitumor efficacy of chemokine CCL5 gene therapy using gene electrotransfer (GET) in combination with radiotherapy (RT) in solid murine tumors CT26 and 4T1. In vitro, CT26 and 4T1 tumor cells transfected with plasmid DNA (pDNA) encoding CCL5 induced migration of RAW264.7 macrophages. In vivo, CCL5 overexpression achieved via GET of pDNA encoding CCL5 led to increased splenocyte infiltration in dorsal window chamber models. When combined with RT, GET of pDNA encoding CCL5 shifted the tumor cytokine profile toward a proinflammatory state, with elevated Ifn-γ, Cxcl9, Cxcl10, and Il-12α. Although CD8 + and CD4 + T cells were reduced post-treatment, due to radiation-induced cell death, the combination of GET of pDNA encoding CCL5 and RT significantly delayed tumor growth in both models. In 4T1 tumors, this delay was also significant compared to the equivalent treatment with GET of control pDNA. These findings support GET of pDNA encoding CCL5 combined with RT as a strategy to enhance immune-mediated tumor control.
Ključne besede: chemokines, gene electrotransfer, gene therapy, mouse, radiotherapy
Objavljeno v DiRROS: 13.02.2026; Ogledov: 339; Prenosov: 47
Celotno besedilo (12,90 MB) |
5. New mitochondrial ▫$K_V$▫1.3 conjugates are potent and specific inducers of apoptosis in cancer modelsŠpela Gubič, Marzia Fois, Ivan Džajić, Katja Kološa, Alja Štern, Maša Omerzel, Tim Božič, Boštjan Markelc, Tanja Jesenko, Maja Čemažar, Bojana Žegura, Tina Kosjek, Andrej Emanuel Cotman, Tihomir Tomašič, Lucija Peterlin-Mašič, 2026, izvirni znanstveni članek Ključne besede: mitochondrial targeting, mitochondrial KV1.3, apoptosis, cancer
Objavljeno v DiRROS: 12.02.2026; Ogledov: 380; Prenosov: 77
Celotno besedilo (7,40 MB)
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7. Kombinirano zdravljenje solidnih tumorjev z intratumoralnim genskim elektroprenosom plazmidne DNA z zapisom za protitelesa anti-CTLA4 in obsevanjemSimona Kranjc Brezar, Boštjan Markelc, Tanja Jesenko, Tim Božič, Paul Declerck, Liesl Jacobs, Maja Čemažar, Kevin Hollevoet, Gregor Serša, 2023, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: solidni tumorji, elektroprenos genov, radioterapija
Objavljeno v DiRROS: 19.06.2023; Ogledov: 1548; Prenosov: 717
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9. Vaskularizacija in vaskularni učinki kot prognostični dejavniki za zdravljenje tumorjev z lokalnimi ablacijskimi tehnikamiTadej Tomanič, Črt Keber, Jošt Stergar, Boštjan Markelc, Tim Božič, Simona Kranjc Brezar, Gregor Serša, Matija Milanič, 2023, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: medicinska fizika, medicinsko slikanje, hiperspektralno slikanje, tumorji, zdravljenje
Objavljeno v DiRROS: 16.06.2023; Ogledov: 1429; Prenosov: 435
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10. Gene electrotransfer of proinflammatory chemokines CCL5 and CCL17 as a novel approach of modifying cytokine expression profile in the tumor microenvironmentTim Božič, Gregor Serša, Simona Kranjc Brezar, Maja Čemažar, Boštjan Markelc, 2021, izvirni znanstveni članek Povzetek: The effectiveness of immunotherapy highly correlates with the degree and the type of infiltrated immune cells in the tumor tissue. Treatments based on modifying the immune cell infiltrate of the tumor microenvironment are thus gaining momentum. Therefore, the aim of our study was to investigate the effects of gene therapy with two proinflammatory chemokines CCL5 and CCL17 on inflammatory cytokine expression profile and immune cell infiltrate in two murine breast tumor models, 4T1 and E0771, and two murine colon tumor models, CT26 and MC38. In vitro, lipofection of plasmid DNA encoding CCL5 or CCL17 resulted in changes in the cytokine expression profile similar to control plasmid DNA, implying that the main driver of these changes was the entry of foreign DNA into the cell%s cytosol. In vivo, gene electrotransfer resulted in high expression levels of both Ccl5 and Ccl17 transgenes in the 4T1 and CT26 tumor models. Besides a minor increase in the survival of the treated mice, the therapy also resulted in increased expression of Cxcl9 and Ifn%, potent activators of the immune system, in CT26 tumors. However, this was not recapitulated in changes of TME, implying that a further refinement of the dosing schedule is needed.
Ključne besede: chemokines, cytokine expression, gene electrotransfer, CCL5
Objavljeno v DiRROS: 19.09.2022; Ogledov: 2045; Prenosov: 633
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