861. Recruitment and controlled growth of juveniles of the critically endangered fan mussel Pinna nobilis in the Northern AdriaticValentina Pitacco, Domen Trkov, Daniela Caracciolo, Saul Ciriaco, Marco Segarich, Edoardo Batistini, Martina Orlando-Bonaca, Lovrenc Lipej, Borut Mavrič, Manja Rogelja, Ilenia Azzena, Chiara Locci, Fabio Scarpa, Daria Sanna, Marco Casu, Chiara Manfrin, Alberto Pallavicini, Ana Fortič, 2025, izvirni znanstveni članek Povzetek: The fan mussel Pinna nobilis is the largest bivalve species in the Mediterranean Sea and provides numerous ecosystem services. It is classified as critically endangered by IUCN (International Union for Conservation of Nature) due to severe mass mortality events throughout the Mediterranean. The aims of this work are as follows: (i) to assess the current recruitment potential of the species, (ii) to enhance recruitment by keeping juveniles in controlled conditions before releasing them back into the sea, and (iii) to assess the health status of recruits. In the period 2022–2023, larval collectors were set up in the Gulf of Trieste as part of the LIFE Pinna project. The collected individuals were kept in aquaria in two different facilities under different conditions: (a) a closed system with constant water temperature, live phytoplankton, and commercial food and (b) an open system with ambient seawater temperature and commercial food. A clear temporal and spatial variability in recruitment was observed: 13 recruits were found in 2022 and 50 recruits in 2023. The live specimens were between 0.5 and 8 cm in size upon collection and larger in 2023. The growth and survival rate did not differ significantly between the two systems, but the average monthly growth and survival rate were related to the initial size of the juveniles.
Ključne besede: pen shell, ex situ maintenance, in situ maintenance, Haplosporidium pinnae, Mycobacterium, conservation, marine biology
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862. Predlog za (so)financiranje ukrepov za gospodarjenje z gozdovi v hudourniških območjih : poročilo izsledka 3.2Urša Vilhar, Jaša Saražin, Matjaž Dovečar, Jože Papež, Erika Kozamernik, 2025, elaborat, predštudija, študija Ključne besede: zakonodaja, gozdarstvo, hudourniki, hudourniška območja, gozdovi
Objavljeno v DiRROS: 08.10.2025; Ogledov: 277; Prenosov: 76
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863. Katalog tehničnih rešitev, ki prispevajo k povečanju erozijske odpornosti v gozdovih : poročilo izsledka 3.3Jaša Saražin, Urša Vilhar, Jože Papež, Matjaž Dovečar, 2025, elaborat, predštudija, študija Ključne besede: zakonodaja, gozdarstvo, hudourniki, hudourniška območja, gozdovi
Objavljeno v DiRROS: 08.10.2025; Ogledov: 321; Prenosov: 117
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864. Deležniki ter njihove pristojnosti in interesi pri gospodarjenju z gozdovi na hudourniških območjih : poročilo izsledka 4.1Urša Vilhar, Jože Papež, Erika Kozamernik, Matjaž Dovečar, 2025, elaborat, predštudija, študija Ključne besede: zakonodaja, gozdarstvo, hudourniki, hudourniška območja, gozdovi
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865. Zakonodajni okvir za gospodarjenje z gozdovi v hudourniških območjih : poročilo izsledka 4.2Urša Vilhar, Jaša Saražin, Matjaž Dovečar, Jože Papež, Erika Kozamernik, 2025, elaborat, predštudija, študija Ključne besede: zakonodaja, gozdarstvo, hudourniki, hudourniška območja, gozdovi
Objavljeno v DiRROS: 08.10.2025; Ogledov: 255; Prenosov: 81
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866. Predlog strokovnih izhodišč za spremembo predpisov s področja gozdarstva za ukrepanje v hudourniških območjih v Sloveniji : poročilo izsledka 4.3Urša Vilhar, Jaša Saražin, Jože Papež, Erika Kozamernik, Matjaž Dovečar, 2025, elaborat, predštudija, študija Ključne besede: zakonodaja, gozdarstvo, hudourniki, hudourniška območja, gozdovi
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867. Effects of manganese dioxide and sintering temperature on the properties and microstructure of a secondary-aluminum-ash ceramic proppantYongming Zeng, Naichen Xiao, Haiping Yang, Peipeng Yang, Fei Yan, Sifeng Bi, Ruien Yu, 2025, izvirni znanstveni članek Ključne besede: ceramic proppant, secondary aluminum ash, crushing rate, microstructure
Objavljeno v DiRROS: 08.10.2025; Ogledov: 315; Prenosov: 132
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868. Resynthesis and electrochemical performance of NCM111 using ultrasound-assisted leachate from spent lithium-ion battery cathodesHonghao Yu, Junhui Ye, Nianping Li, Xin Li, 2025, izvirni znanstveni članek Ključne besede: spent lithium-ion batteries, ultrasound-assisted leachate, resynthesis, deep eutectic solvent, LiNi1/3Co1/3Mn1/3O2
Objavljeno v DiRROS: 08.10.2025; Ogledov: 239; Prenosov: 116
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869. Improving biodiversity in Central and Eastern European gardens needs regionally scaled strategiesZsófia Varga-Szilay, Arvids Barševskis, Klára Benedek, Danilo Bevk, 2025, izvirni znanstveni članek Povzetek: Amid ongoing urbanisation, gardens are expected to play an increasing role in enhancing urban biodiversity by supplementing green areas and improving landscape connectivity. Biodiversity-friendly gardens also improve human well-being and foster connections between nature and people. To study these benefits, we distributed a questionnaire (n = 5255), and used a scoring system to evaluate gardens’ ecological value (GAR index), gardeners' attitudes (RES index), and pesticide use habits (PES index). We used machine learning to explore how these indices interact and what sociodemographic factors drive them across Central and Eastern Europe (CEE). Our aim was to explore the ecological values of gardens and gardening practices, identifying characteristics that might contribute to building high biodiversity. We found significant variability within and between countries, with Romania scoring low and Czechia high in all indices. Domestic pesticide use was ubiquitous across CEE and largely unaffected by sociodemographic factors. Increased time spent gardening was associated with the highest pesticide use and a greater potential for fostering high biodiversity. Gardeners aged over 55 tended to uphold longstanding conventional practices and thus lowered both PES and GAR index scores. The local differences highlight the need for regionally tailored biodiversity-friendly gardening guidelines instead of standardised regulations across Europe. Effective environmental education and community programs can be developed based on local biodiversity and the three indices we used. These programs should inform gardeners about the environmental and health impacts of pesticides and provide comprehensive biodiversity-related knowledge. This is especially important in CEE, where such initiatives are currently underrepresented.
Ključne besede: rural-urban gradient, urban ecosystems, environmental consciousness, sustainable gardening, environmental sensitivity, urbanisation
Objavljeno v DiRROS: 08.10.2025; Ogledov: 203; Prenosov: 99
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870. From vineyard to genome : optimized enrichment and sequencing of Flavescence dorée phytoplasma from grapevine samplesZala Kogej Zwitter, Denis Kutnjak, Nataša Mehle, 2025, izvirni znanstveni članek Povzetek: Phytoplasmas are non-culturable obligate intracellular bacteria that cause considerable economic losses in agriculture. Genome sequencing provides crucial insights into their biology and vector dependence. However, genome studies on phytoplasmas are often hampered by their low abundance in naturally infected plants. Propagation in test plants is usually necessary but time-consuming and resource-intensive, especially for quarantine phytoplasmas such as the phytoplasma causing Flavescence dorée (FD), a serious threat to European viticulture. To overcome these challenges, we aimed to develop a protocol for efficient enrichment of phytoplasma DNA directly from field-collected samples, enabling genome sequencing using both Illumina and Oxford Nanopore Technologies platforms. We evaluated six sample preparation protocols that included stepwise enrichment steps to improve phytoplasma genome coverage and assembly quality. The most effective approach combined differential centrifugation, CTAB extraction and removal of CpG-methylated host DNA and resulted in a notable increase in the relative abundance of phytoplasma reads compared to other protocols. Rarefaction analysis of the dataset generated using this protocol demonstrated that the entire phytoplasma genome was covered by reads in a dataset comprising 3 billion nucleotides. We also evaluated and compared de novo phytoplasma genome assemblies generated from short Illumina reads and long nanopore sequencing reads. While Illumina sequencing yielded more accurate assemblies with longer total lengths, the assemblies derived from nanopore sequencing data contained longer individual contigs. This advantage was reflected in hybrid assemblies that combined both technologies, yielding longer phytoplasma contigs than assemblies from Illumina datasets and lower mismatch rates compared to assemblies from nanopore sequencing datasets. A hybrid de novo assembled genome of the Slovenian FD phytoplasma isolate achieved 96% reference genome coverage, with high contiguity and low error rates. This streamlined and accessible protocol enables high-quality genome sequencing of phytoplasma-infected grapevines without the need for propagation in test plants. This facilitates broader phytoplasma research and can potentially be extended to other naturally infected phytoplasma hosts or organisms infected with other non-culturable microbes.
Ključne besede: plant pathogen, nanopore, grapevine, enrichment, HTS, Flavescence dorée, phytoplasma
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