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1102. A fuzzy hypergraph-based framework for secure encryption and decryption of sensitive messages : application in selecting center location to set up a private hospitalAnnamalai Meenakshi, Obel Mythreyi, Leo Mršić, Antonios Kalampakas, Sovan Samanta, 2025, izvirni znanstveni članek Ključne besede: encryption, decryption, fuzzy hypergraph, dual fuzzy hypergraph, secure network
Objavljeno v DiRROS: 09.09.2025; Ogledov: 302; Prenosov: 135
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1107. Magnetomechanical detachment of bacterial biofilms using anisotropic magnetic iron oxide nanochainsMatija Šavli, Manca Černila, Maja Caf, Abida Zahirović, Nika Zaveršek, Sebastjan Nemec, Spase Stojanov, Anja Klančnik, Jerica Sabotič, Slavko Kralj, Aleš Berlec, 2025, izvirni znanstveni članek Povzetek: Bacterial biofilms attach to various surfaces and represent an important clinical and public health problem, as they are highly recalcitrant and are often associated with chronic, nonhealing diseases and healthcare-associated infections. Antibacterial agents are often not sufficient for their elimination and have to be combined with mechanical removal. Mechanical forces can be generated by actuating nonspherical (anisotropic) magnetically responsive nanoparticles in a rotating magnetic field. We have thus prepared anisotropic superparamagnetic nanochains in the size range of 0.5−1 μm by magnetically assembling several iron oxide nanoparticle clusters and coating them with a layer of silica with different shell morphologies: smooth, moderately rough, and highly rough. The silica surface was additionally functionalized with carboxylic groups to increase colloidal stability. The efficacy of the nanochains in biofilm removal was studied systematically with three different model nonpathogenic bacterial species Escherichia coli, Lactococcus lactis, and Pseudomonas fragi; two different magnetic field strengths; two stirring speeds; and two treatment durations. All bacterial species were engineered to express fluorescent proteins to enable quantification of biofilm removal by colony-forming unit count and fluorescence measurements. Nanochains removed >90% of Gram-negative E. coli and P. fragi with a stronger magnetic field, and <90% of Gram-positive L. lactis with a weaker magnetic field. Surface roughness of nanochains, duration, and stirring speed also affected removal, but the effect could not be generalized. In contrast to their effects on biofilms, the functionalized nanochains showed no toxicity to Caco-2 intestinal epithelial cells, regardless of whether magnetomechanical force was employed or not. In summary, we demonstrated that remotely controlled spatial movement of nanoparticles can generate sufficient mechanical forces to disperse attached biofilms while retaining safety in an epithelial cell model.
Ključne besede: bacterial biofilm, magnetomechanical detachment, magnetic nanoparticles, nanochains
Objavljeno v DiRROS: 09.09.2025; Ogledov: 299; Prenosov: 137
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1110. Overcoming biases in opportunistic citizen science for studying life history traits of an invasive leaf-mining tree insect pestNatalia I. Kirichenko, Maria A. Ryazanova, Oksana V. Kosheleva, Stanislav Gomboc, Barbara Piškur, Maarten De Groot, 2025, izvirni znanstveni članek Povzetek: The aim of this study was to determine whether opportunistic citizen science can support the detection of life history traits in invasive insects. Using the invasive leaf-mining micromoth Macrosaccus robiniella (Clemens 1859) (Lepidoptera: Gracillariidae) as a model species, we analyzed data from iNaturalist submitted by citizen scientists to assess the variability in its leaf mines on its native host, Robinia pseudoacacia L., 1753 (Fabaceae), across both the moth’s invaded (Europe, North America–Eastern United States) and native range (North America–Southern and Western Unites States, Eastern Canada). We examined 86,489 photographs collected over the past 20 years to compare the occurrence and proportions of different M. robiniella leaf mine types between invaded and native ranges using three search variants: (I) M. robiniella, (II) all endophagous invasive insects associated with R. pseudoacacia, and (III) the host plant itself. The first two datasets revealed differences in the ratio of leaf mine types between Europe and North America (when analyzed separately for native and invaded areas), whereas the third dataset showed no significant differences in either the presence or proportion of mine types between invaded and native ranges. Leaf mine types atypical of M. robiniella, which resemble damage caused by other invasive insects such as Parectopa robiniella Clemens, 1863 (Lepidoptera: Gracillariidae) and Obolodiplosis robiniae (Haldeman, 1847) (Diptera: Cecidomyiidae)—also associated with R. pseudoacacia—have been observed in Europe for at least a decade. Our main conclusion is that, when investigating the life history traits of invasive herbivorous insects, focusing data collection on the host plant rather than on the insect species alone can reduce biases associated with opportunistic citizen science and help reveal true ecological patterns.
Ključne besede: iNaturalist, invasive leaf miner, Macrosaccus robiniella, leaf mine types, Europe, North America
Objavljeno v DiRROS: 09.09.2025; Ogledov: 262; Prenosov: 118
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