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Povzetek: The ocean, including its coastal areas and covering more than 70% of the Earth’s surface, has always represented an important environmental and economic resource. Indeed, almost 40% of the global population lives in coastal communities (United Nations, 2017). With its ecosystem services, the ocean represents a pivotal role in human society (Rayner et al., 2019). Undeniably, the ocean provides food, regulates the climate, provides oxygen and ensures economic resources through its shipping routes and tourism opportunities. Additionally, the ocean is home to organisms that have for centuries sparked the scientific interest of many research groups to uncover the biodiversity and functions of these fascinating marine ecosystems. Through their biological and chemical diversity, marine organisms synthesize unique secondary metabolites, biopolymers and enzymes produced in response to environmental stimuli. Secondary metabolites play important biological roles in improving competitiveness, providing chemical defence against predators or competitors and facilitating reproductive processes (Rotter et al.). Screening of these natural products and their producer organisms, coupled with the search of their unique biological activities that could be used in various industries, is tackled within marine (blue) biotechnology. Marine organisms and microorganisms can be investigated, and their primary and secondary metabolites, biopolymers and enzymes can be used as lead agents for nutraceutical and pharmaceutical industries to improve processes (e.g., in drug delivery) and as a source of bio-inspired materials for numerous biotechnological applications. Although this field has been appearing since the 1960s and 1970s, it is still considered an emerging field and marine biotechnology is still in its infancy (Rayner et al., 2019; Rotter et al.). This is because many marine environments are extreme ones that are either hardly accessible for sampling and harvesting and/or are home to organisms that cannot be cultured or grown in laboratory conditions. Consequently, a lot of advancement in the field of marine biotechnology was hampered until recent advances in science were achieved, including sampling methods, high-throughput methods and transdisciplinary collaborations.
Ključne besede: blue biotechnology, marine biotechnology, ocean bioprospecting, marine organisms
Objavljeno v DiRROS: 06.08.2024; Ogledov: 32; Prenosov: 18
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Povzetek: Wastewater treatment plants (WWTPs) are a focal point for the removal of microplastic (MP) particles before they are discharged into aquatic environments. WWTPs are capable of removing substantial quantities of larger MP particles but are inefficient in removing particles with any one dimension of less than 100 μm, with influents and effluents tending to have similar quantities of these smaller particles. As a single WWTP may release >100 billion MP particles annually, collectively WWTPs are significant contributors to the problem of MP pollution of global surface waters. Currently, there are no policies or regulations requiring the removal of MPs during wastewater treatment, but as concern about MP pollution grows, the potential for wastewater technologies to capture particles before they reach surface waters has begun to attract attention. There are promising technologies in various stages of development that may improve the removal of MP particles from wastewater. Better incentivization could speed up the research, development and adoption of innovative practices. This paper describes the current state of knowledge regarding MPs, wastewater and relevant policies that could influence the development and deployment of new technologies within WWTPs. We review existing technologies for capturing very small MP particles and examine new developments that may have the potential to overcome the shortcomings of existing methods. The types of collaborations needed to encourage and incentivize innovation within the wastewater sector are also discussed, specifically strong partnerships among scientific and engineering researchers, industry stakeholders, and policy decision makers.
Ključne besede: wastewater, microplastic, particle removal, innovation, policy, jellyfish mucus
Objavljeno v DiRROS: 06.08.2024; Ogledov: 35; Prenosov: 30
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Povzetek: Biomass is defined as organic matter from living organisms represented in all kingdoms. It is recognized to be an excellent source of proteins, polysaccharides and lipids and, as such, embodies a tailored feedstock for new products and processes to apply in green industries. The industrial processes focused on the valorization of terrestrial biomass are well established, but marine sources still represent an untapped resource. Oceans and seas occupy over 70% of the Earth’s surface and are used intensively in worldwide economies through the fishery industry, as logistical routes, for mining ores and exploitation of fossil fuels, among others. All these activities produce waste. The other source of unused biomass derives from the beach wrack or washed-ashore organic material, especially in highly eutrophicated marine ecosystems. The development of high-added-value products from these side streams has been given priority in recent years due to the detection of a broad range of biopolymers, multiple nutrients and functional compounds that could find applications for human consumption or use in livestock/pet food, pharmaceutical and other industries. This review comprises a broad thematic approach in marine waste valorization, addressing the main achievements in marine biotechnology for advancing the circular economy, ranging from bioremediation applications for pollution treatment to energy and valorization for biomedical applications. It also includes a broad overview of the valorization of side streams in three selected case study areas: Norway, Scotland, and the Baltic Sea.
Objavljeno v DiRROS: 05.08.2024; Ogledov: 51; Prenosov: 54
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Povzetek: Soil salinity and drought are among the most serious agricultural and environmental problems of today. Therefore, investigations of plant resistance to abiotic stress have received a lot of attention in recent years. In this study, we identified the complete coding sequence of a 3′-phosphoadenosine-5′-phosphatase protein, ApHal2, from the halotolerant yeast Aureobasidium pullulans. Expression of the ApHAL2 gene in a Saccharomyces cerevisiae hal2 mutant complemented the mutant auxotrophy for methionine, and rescued the growth of the hal2 mutant in media with high NaCl concentrations. A 21-amino-acids-long region of the ApHal2 enzyme was inserted into the Arabidopsis thaliana homologue of Hal2, the SAL1 phosphatase. The inserted sequence included the META motif, which has previously been implicated in increased sodium tolerance of the Hal2 homologue from a related fungal species. Transgenic Arabidopsis plants overexpressing this modified SAL1 (mSAL1) showed improved halotolerance and drought tolerance. In a medium with an elevated salt concentration, mSAL1-expressing plants were twice as likely to have roots in a higher length category in comparison with the wild-type Arabidopsis and with plants overexpressing the native SAL1, and had 5% to 10% larger leaf surface area under moderate and severe salt stress, respectively. Similarly, after moderate drought exposure, the mSAL1-expressing plants showed 14% increased dry weight after revitalisation, with no increase in dry weight of the wild-type plants. With severe drought, plants overexpressing native SAL1 had the worst rehydration success, consistent with the recently proposed role of SAL1 in severe drought. This was not observed for plants expressing mSAL1. Therefore, the presence of this fungal META motif sequence is beneficial under conditions of increased salinity and moderate drought, and shows no drawbacks for plant survival under severe drought. This demonstrates that adaptations of extremotolerant fungi should be considered as a valuable resource for improving stress-tolerance in plant breeding in the future.
Ključne besede: soil salinity and drought, plant resistance, abiotic stress
Objavljeno v DiRROS: 02.08.2024; Ogledov: 119; Prenosov: 106
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Povzetek: In the field, plants are challenged by more than one biotic stressor at the same time. In this study, the molecular interactions between potato (Solanum tuberosum L.), Colorado potato beetle (Leptinotarsa decemlineata Say; CPB) and Potato virus YNTN (PVYNTN) were investigated through analyses of gene expression in the potato leaves and the gut of the CPB larvae, and of the release of potato volatile compounds. CPB larval growth was enhanced when reared on secondary PVYNTN-infected plants, which was linked to decreased accumulation of transcripts associated with the antinutritional properties of potato. In PVYNTN-infected plants, ethylene signalling pathway induction and induction of auxin response transcription factors were attenuated, while no differences were observed in jasmonic acid (JA) signalling pathway. Similarly to rearing on virus-infected plants, CPB larvae gained more weight when reared on plants silenced in JA receptor gene (coi1). Although herbivore-induced defence mechanism is regulated predominantly by JA, response in coi1-silenced plants only partially corresponded to the one observed in PVYNTN-infected plants, confirming the role of other plant hormones in modulating this response. The release of β-barbatene and benzyl alcohol was different in healthy and PVYNTN-infected plants before CPB larvae infestation, implicating the importance of PVYNTN infection in plant communication with its environment. This was reflected in gene expression profiles of neighbouring plants showing different degree of defence response. This study thus contributes to our understanding of plant responses in agro-ecosystems.
Ključne besede: insect midgut transcriptional response, gene expression, plant defence, volatile organic compounds, potato
Objavljeno v DiRROS: 02.08.2024; Ogledov: 84; Prenosov: 58
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