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Povzetek: Background: High-grade serous carcinoma (HGSC) is often associated with ascites at presentation. Our objective was to quantify immune cells (ICs) in ascites prior to any treatment was given and evaluate their impact on progression-free survival (PFS) and overall survival (OS). Patients and methods: Forty-seven patients with primary HGSC and ascites were included. Flow-cytometric analysis was performed to detect percentages of CD3+ T cells (CD4+, CD8+, Tregs, and NKT cells), B cells, NK cells (CD56brightCD16- and CD56dimCD16+ subsets), macrophages and dendritic cells (DCs). Furthermore, CD103 expression was analyzed on T cells and their subsets, while PD-1 and PD-L1 expression on all ICs. Cut-off of low and high percentages of ICs was determined by the median of variables, and correlation with PFS and OS was calculated. Results: CD3+ cells were the predominant ICs (median 51%), while the presence of other ICs was much lower (median ≤10%). CD103+ expression was mostly present on CD8+, and not CD4+ cells. PD-1 was mainly expressed on CD3+ T cells (median 20%), lower expression was observed on other ICs (median ≤10%). PD-L1 expression was not detected. High percentages of CD103+CD3+ T cells, PD-1+ Tregs, CD56brightCD16- NK cells, and DCs correlated with prolonged PFS and OS, while high percentages of CD8+ cells, macrophages, and PD-1+CD56brightCD16- NK cells, along with low percentages of CD4+ cells, correlated with better OS only. DCs were the only independent prognostic marker among all ICs. Conclusions: Our results highlight the potential of ascites tumor-immune microenvironment to provide additional prognostic information for HGSC patients. However, a larger patient cohort and longer follow-up are needed to confirm our finBackground: High-grade serous carcinoma (HGSC) is often associated with ascites at presentation. Our objective was to quantify immune cells (ICs) in ascites prior to any treatment was given and evaluate their impact on progression-free survival (PFS) and overall survival (OS). Patients and methods: Forty-seven patients with primary HGSC and ascites were included. Flow-cytometric analysis was performed to detect percentages of CD3+ T cells (CD4+, CD8+, Tregs, and NKT cells), B cells, NK cells (CD56brightCD16- and CD56dimCD16+ subsets), macrophages and dendritic cells (DCs). Furthermore, CD103 expression was analyzed on T cells and their subsets, while PD-1 and PD-L1 expression on all ICs. Cut-off of low and high percentages of ICs was determined by the median of variables, and correlation with PFS and OS was calculated. Results: CD3+ cells were the predominant ICs (median 51%), while the presence of other ICs was much lower (median ≤10%). CD103+ expression was mostly present on CD8+, and not CD4+ cells. PD-1 was mainly expressed on CD3+ T cells (median 20%), lower expression was observed on other ICs (median ≤10%). PD-L1 expression was not detected. High percentages of CD103+CD3+ T cells, PD-1+ Tregs, CD56brightCD16- NK cells, and DCs correlated with prolonged PFS and OS, while high percentages of CD8+ cells, macrophages, and PD-1+CD56brightCD16- NK cells, along with low percentages of CD4+ cells, correlated with better OS only. DCs were the only independent prognostic marker among all ICs. Conclusions: Our results highlight the potential of ascites tumor-immune microenvironment to provide additional prognostic information for HGSC patients. However, a larger patient cohort and longer follow-up are needed to confirm our findings.dings.
Ključne besede: high-grade serous carcinoma, immune cells, prognostic markers
Objavljeno v DiRROS: 26.07.2024; Ogledov: 616; Prenosov: 259
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Ključne besede: optical coherence tomography, angiography, endoscopy, oncology, skin carcinoma
Objavljeno v DiRROS: 26.07.2024; Ogledov: 546; Prenosov: 225
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Povzetek: The data regarding the occurrence of the Cline (Clinitrachus argentatus) in the Adriatic Sea are scarce, limited and sporadic. In the Slovenian sea only three specimens were observed in the past decades. The paper presents new findings of the species from 2013 to 2016, when some specimens were occasionally manually collected in shallow waters. Data about habitat preferences, depth range, total lengths and meristic counts are reported. The aim of the study is also to discuss the reasons for the underestimation of the occurrence of C. argentatus in the area.
Ključne besede: fishes, shallow waters, overlooked species, habitat preferences, Adriatic Sea
Objavljeno v DiRROS: 26.07.2024; Ogledov: 352; Prenosov: 190
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Povzetek: A juvenile male of porbeagle (Lamna nasus) was caught in waters off Piran (Slovenia, northern Adriatic) on December 22, 2015. The specimen was accurately measured and weighed. Cephalopods and fish remains were found in its stomach contents. This is the first record of a porbeagle in the waters of Slovenia and in the Gulf of Trieste, and one of the few records up to date reported in the northern Adriatic Sea. The presence of this juvenile specimen arises a question whether the Adriatic Sea is a reproductive ground of this species. According to an older record from 1910, a female porbeagle with four embryos was caught on Ugljan Island, confirming the fact that porbeagles reproduced at least in the past in the Adriatic Sea.
Ključne besede: sharks, lamnoid sharks, by-catch, occurrence, Adriatic Sea
Objavljeno v DiRROS: 26.07.2024; Ogledov: 323; Prenosov: 168
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Povzetek: We have studied the performances of (a) a two-way coupled atmosphere%ocean modeling system and (b) one-way coupled ocean model (forced by the atmosphere model), as compared to the available in situ measurements during and after a strong Adriatic bora wind event in February 2012, which led to extreme air%sea interactions. The simulations span the period between January and March 2012. The models used were ALADIN (Aire Limitée Adaptation dynamique Développement InterNational) (4.4 km resolution) on the atmosphere side and an Adriatic setup of Princeton ocean model (POM) (1%=30%1%=30 angular resolution) on the ocean side. The atmosphere%ocean coupling was implemented using the OASIS3-MCT model coupling toolkit. Two-way coupling ocean feedback to the atmosphere is limited to sea surface temperature. We have compared modeled atmosphere%ocean fluxes and sea temperatures from both setups to platform and CTD (conductivity, temperature, and depth) measurements from three locations in the northern Adriatic.We present objective verification of 2m atmosphere temperature forecasts using mean bias and standard deviation of errors scores from 23 meteorological stations in the eastern part of Italy. We show that turbulent fluxes from both setups differ up to 20° during the bora but not significantly before and after the event. When compared to observations, two-way coupling ocean temperatures exhibit a 4 times lower root mean square errors (RMSE) than those from one-way coupled system. Two-way coupling improves sensible heat fluxes at all stations but does not improve latent heat loss. The spatial average of the two-way coupled atmosphere component is up to 0.3 °C colder than the one-way coupled setup, which is an improvement for prognostic lead times up to 20 h. Daily spatial average of the standard deviation of air temperature errors shows 0.15 °C improvement in the case of coupled system compared to the uncoupled. Coupled and uncoupled circulations in the northern Adriatic are predominantly wind-driven and show no significant mesoscale differences.
Ključne besede: sea, marine water, numerical modeling, physical oceanography, dense water, bora wind, Adriatic sea, Mediterranean sea, Adriatic shelf
Objavljeno v DiRROS: 26.07.2024; Ogledov: 373; Prenosov: 225
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