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111.
112.
Candidate pathogenicity factor/effector proteins of ‘Candidatus Phytoplasma solani’ modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomes
Marina Dermastia, Špela Tomaž, Rebeka Strah, Tjaša Lukan, Anna Coll Rius, Barbara Dušak, Timotej Čepin, Aleš Kladnik, Maja Zagorščak, Kristina Gruden, Maruša Pompe Novak, 2023, izvirni znanstveni članek

Povzetek: The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ‘Candidiatus Phytoplasma solani’ are unknown. Six putative pathogenicity factors/effectors from six different strains of ‘Ca. P. solani’ were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate–glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate–glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed.
Ključne besede: autophagosome, effector, glycolysis, pathogenicity factor, StAMP
Objavljeno v DiRROS: 24.08.2023; Ogledov: 508; Prenosov: 239
.pdf Celotno besedilo (7,84 MB)
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113.
Tribocorrosive study of new and in vivo exposed nickel titanium and stainless steel orthodontic archwires
Tadeja Kosec, Petra Močnik, Uroš Mezeg, Andraž Legat, Maja Ovsenik, Monika Jenko, John T. Grant, Jasmina Primožič, 2020, izvirni znanstveni članek

Povzetek: The surface, corrosion and wear properties of new and in vivo exposed nickel titanium (NiTi) and stainless steel (SS) archwires used in orthodontic treatment were investigated. Electrochemical and tribo-electrochemical tests in artificial saliva were performed in order to define corrosion properties and to estimate wear rate of new and in vivo exposed NiTi and SS archwires. The surface chemical analysis of the passive film on the NiTi and SS archwires before and after tribocorrosion tests was performed by Auger Electron Spectroscopy (AES). In vivo exposed NiTi and SS archwires had better electrochemical properties than new archwires due to the protective nature of oral deposits. Total wear and coefficients of friction were higher among in vivo exposed archwires and higher in NiTi archwires in comparison to SS archwires. The estimated thickness of the TiO2 passive film on as-received NiTi is 8 nm, while the passive Cr2O3 film on as-received SS is just 1–2 nm. On in vivo exposed NiTi archwire, a 60–80 nm thick organic film/dental plaque was observed, and on SS, it was thinner, at about 60 nm. This research shows the importance of combining AES with electrochemical testing, to characterize tribocorrosive properties of NiTi and SS orthodontic archwires.
Ključne besede: archwires, NiTi, stainless steel, wear
Objavljeno v DiRROS: 24.08.2023; Ogledov: 290; Prenosov: 127
.pdf Celotno besedilo (2,18 MB)
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114.
Biokemijske analize (API ZYM testi) dominantnih parazitskih in saprofitskih gliv črnega bora (Pinus nigra Arn.)
Maja Jurc, Nada Gogala, 1996, izvirni znanstveni članek

Povzetek: Prispevek obravnava uporabo nekaterih biokemijskih analiz (API ZYM testov) pri pojasnjevanju vloge petih dominantnih endofitnih gliv (Cyclaneusma niveum (Pers.:Fr.) DiCosmo, Perdo & Minter, Cenangium ferruginosum Fr., Phialophora hoffmannii (Beyma) Schol-Schwarz, Cyclaneusma minus (Butin) DiCosmo, Perdo & Minter ter Hormonema dematioides Lagerberg & Melin), izoliranih iz iglic črnega bora. Ugotovljena je podobnost intracelularne in ekstarcelularne encimske aktivnosti analiziranih gliv. Pri vseh glivah je ugotovljena lipolitska aktivnost. Biokemijske analize podgobja lahko le delno nakažejo, v katero prehranjevalno skupino uvrščamo glivo (parazit, šibek parazit, saprofit)
Ključne besede: endofitne glive, črni bor, Pinus nigra Arn., encimi, latentna okužba, patogenost
Objavljeno v DiRROS: 23.08.2023; Ogledov: 470; Prenosov: 116
.pdf Celotno besedilo (1,65 MB)

115.
116.
A red Roman column from Emona (Ljubljana, Slovenia)
Andreja Maver, Bernarda Županek, Maja Gutman, Dragomir Skaberne, Sabina Dolenec, 2020, izvirni znanstveni članek

Povzetek: The excavation conducted in 1999 in Ljubljana (Slovenia), in Insula XXVII of the Roman colonia Iulia Emona, un-earthed the remains of a column shaft with an organic core set onto a stone block. The contribution presents this find, its context and likely parallels, the mineralogical-petrographic composition of the stucco, type of the pigments used, as well as the rock of the plinth and its provenance. Of the shaft, only the stucco survives, applied in four mortar layers and painted in red ochre, while its interior surface shows the impressions of reeds. The plinth is a rectangular block made of locally available Lower Jurassic oolitic limestone, presumably from Podpeč. The column may have formed part of a porticus or peristyle, possibly associated with a pool or a bath complex in this part of Late Roman Emona.
Ključne besede: NUK II Site (Ljubljana, Slovenia), columns, Roman architecture, reed impressions, stucco, limestone, Late Roman period
Objavljeno v DiRROS: 04.08.2023; Ogledov: 337; Prenosov: 236
.pdf Celotno besedilo (12,00 MB)
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117.
118.
Priporočila za obravnavo bolnikov s pljučnim rakom
Martina Vrankar, Nina Boc, Izidor Kern, Aleš Rozman, Karmen Stanič, Tomaž Štupnik, Mojca Unk, Maja Ebert Moltara, Vesna Zadnik, Katja Adamič, Jernej Benedik, Marko Bitenc, Jasna But-Hadžić, Anton Crnjac, Marina Čakš, Dominik Časar, Eva Ćirić, Tanja Čufer, Ana Demšar, Rok Devjak, Goran Gačevski, Marta Globočnik Kukovica, Kristina Gornik-Kramberger, Maja Ivanetič Pantar, Marija Ivanović, Urška Janžič, Staša Jelerčič, Veronika Kloboves-Prevodnik, Mile Kovačević, Luka Ležaič, Mateja Marc-Malovrh, Katja Mohorčič, Loredana Mrak, Igor Požek, Nina Turnšek, Bogdan Vidmar, Dušanka Vidovič, Gregor Vlačić, Ana Lina Vodušek, Rok Zbačnik, Ivana Žagar, 2023, strokovni članek

Povzetek: Leta 2019 so bila objavljena Priporočila za obravnavo bolnikov s pljučnim rakom, ki so v slovenski prostor vnesla prepotrebno poenotenje diagnostike in zdravljenja z namenom izboljšanja preživetja bolnikov s pljučnim rakom. Posodobitev Priporočil tri leta po izidu izvirnika prinaša največ novosti v poglavju o sistemskem zdravljenju bolnikov s pljučnim rakom. To kaže na izjemen napredek na področju razumevanja onkogeneze in biologije pljučnega raka ter s tem razvoja novih zdravil. Breme pljučnega raka ostaja veliko, saj je pljučni rak pri nas in v svetu še vedno najpogostejši vzrok smrti zaradi raka. Za vsako peto smrt zaradi raka je odgovoren pljučni rak. Skoraj tretjina bolnikov s pljučnim rakom ne prejme specifičnega onkološkega zdravljenja, bodisi zaradi slabega stanja zmogljivosti, spremljajočih bolezni ali obsega bolezni. Polovica bolnikov ima ob diagnozi razsejano bolezen, zaradi česar izboljšanje preživetja z malimi koraki sledi napredku v zdravljenju bolnikov s pljučnim rakom. Ti podatki nas opominjajo, da se bomo morali za velike premike v obravnavi bolnikov s pljučnim rakom lotiti drugačnih pristopov. Kot najbolj obetavno se ponuja zgodnje odkrivanje bolezni, ko so možnosti ozdravitve pljučnega raka najboljše. Zapisana Priporočila so usmeritev za obravnavo bolnikov s pljučnim rakom. Le s sodobnim multidisciplinarnim pristopom obravnave lahko bolniku ponudimo zdravljenje, ki mu omogoča najboljši izhod prognostično neugodne bolezni.
Ključne besede: pljučni rak, priporočila
Objavljeno v DiRROS: 27.07.2023; Ogledov: 473; Prenosov: 164
.pdf Celotno besedilo (708,18 KB)
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119.
Potato (Solanum tuberosum) - a new host for the root-knot nematode Meloidogyne inornata
Barbara Gerič Stare, Nik Susič, Maja Starovič, Saša Širca, 2022, drugi znanstveni članki

Ključne besede: pathogenicity, plant-parasitic nematodes, nematodes, root-knot nematodes, potato
Objavljeno v DiRROS: 27.07.2023; Ogledov: 385; Prenosov: 156
.pdf Celotno besedilo (615,48 KB)
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120.
Measurements of bridge dynamic amplification factor using bridge weigh-in-motion data
Jan Kalin, Aleš Žnidarič, Andrej Anžlin, Maja Kreslin, 2022, izvirni znanstveni članek

Povzetek: The dynamic component of bridge traffic loading is commonly taken into account with a Dynamic Amplification Factor (DAF)—the ratio between the dynamic and static load effects on a bridge. In the design codes, this factor is generally more conservative than in reality. Recently a new method of cal- culation of this factor had been developed. Data from 15 different bridges have been analysed since then and this paper presents the results of the analyses. The background for Bridge Weigh-in-Motion is given, and the most recent method for DAF calculation is described. The sites from which the data originated are presented, and the selection of data discussed. The results of the analyses are presented and discussed and some examples of DAF calculations are shown. Data from the considered sites have invariably demonstrated a DAF decrease with increasing axle load. This is a significant result, especially for assessment of existing structures, since it is beneficial to use measured structural param- eters to optimise structural analysis.
Ključne besede: bridge loads, bridge weigh-in-motion, dynamic amplication factor, dynamic analysis, measurement, traffic loading
Objavljeno v DiRROS: 14.07.2023; Ogledov: 341; Prenosov: 178
.pdf Celotno besedilo (2,61 MB)
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