181. Estimating quantitative physiological and morphological tissue parameters of murine tumor models using hyperspectral imaging and optical profilometryTadej Tomanič, Luka Rogelj, Jošt Stergar, Boštjan Markelc, Tim Božič, Simona Kranjc Brezar, Gregor Serša, Matija Milanič, 2022, izvirni znanstveni članek Povzetek: Understanding tumors and their micro-environment are essential for successfuland accurate disease diagnosis. Tissuephysiology and morphology are altered intumors compared to healthy tissues, andthere is a need to monitor tumors and their surrounding tissues, includingblood vessels, non-invasively. This preliminary study utilizes a multimodaloptical imaging system combining hyperspectral imaging (HSI) and three-dimensional (3D) optical profilometry (OP) to capture hyperspectral imagesand surface shapes of subcutaneously grown murine tumor models. Hyper-spectral images are corrected with 3D OP data and analyzed using the inverse-adding doubling (IAD) method to extract tissue properties such as melaninvolume fraction and oxygenation. Blood vessels are segmented using theB-COSFIRE algorithm from oxygenation maps. From 3D OP data, tumor vol-umes are calculated and compared to manual measurements using a verniercaliper. Results show that tumors can be distinguished from healthy tissuebased on most extracted tissue parameters (p<0:05). Furthermore, blood oxy-genation is 50% higher within the blood vessels than in the surrounding tissue,and tumor volumes calculated using 3D OP agree within 26% with manualmeasurements using a vernier caliper. Results suggest that combining HSI andOP could provide relevant quantitative information about tumors and improvethe disease diagnosis.
Ključne besede: medical physics, hyperspectral imaging, diffuse reflectance spectroscopy, blood vessels, tumors
Objavljeno v DiRROS: 08.09.2022; Ogledov: 593; Prenosov: 199
 Celotno besedilo (3,79 MB) |
182. Non-clinical in vitro evaluation of antibiotic resistance gene-free plasmids encoding human or murine IL-12 intended for first-in-human clinical studyŠpela Kos, Maša Omerzel, Tanja Jesenko, Boštjan Markelc, Urška Kamenšek, Katarina Žnidar, Urška Matkovič, Andrej Renčelj, Gregor Serša, Rosana Hudej, Aneja Tuljak, Matjaž Peterka, Maja Čemažar, 2021, izvirni znanstveni članek Povzetek: Interleukin 12 (IL-12) is a key cytokine that mediates antitumor activity of immune cells. To fulfill its clinical potential, the development is focused on localized delivery systems, such as gene electrotransfer, which can provide localized delivery of IL-12 to the tumor microenvironment. Gene electrotransfer of the plasmid encoding human IL-12 is already in clinical trials in USA, demonstrating positive results in the treatment of melanoma patients. To comply with EU regulatory requirements for clinical application, which recommend the use of antibiotic resistance gene-free plasmids, we constructed and developed the production process for the clinical grade quality antibiotic resistance gene-free plasmid encoding human IL-12 (p21-hIL-12-ORT) and its ortholog encoding murine IL-12 (p21-mIL-12-ORT). To demonstrate the suitability of the p21-hIL-12-ORT or p21-mIL-12-ORT plasmid for the first-in-human clinical trial, the biological activity of the expressed transgene, its level of expression and plasmid copy number were determined in vitro in the human squamous cell carcinoma cell line FaDu and the murine colon carcinoma cell line CT26. The results of the non-clinical evaluation in vitro set the basis for further in vivo testing and evaluation of antitumor activity of therapeutic molecules in murine models as well as provide crucial data for further clinical trials of the constructed antibiotic resistance gene-free plasmid in humans.
Ključne besede: interleukin 12, gene electrotransfer, antibiotic resistance, plasmids
Objavljeno v DiRROS: 07.09.2022; Ogledov: 541; Prenosov: 309
Celotno besedilo (4,73 MB)
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183. Influence of housing conditions on reliability of immunocastration and consequences for growth performance of male pigsKevin Kress, Ulrike Weiler, Sonja Schmucker, Marjeta Čandek-Potokar, Milka Vrecl, Gregor Fazarinc, Martin Škrlep, Nina Batorek Lukač, Volker Stefanski, 2020, izvirni znanstveni članek Povzetek: Immunocastration is a sustainable alternative to piglet castration but faces limited market acceptance. The phenomenon of non-responders has not to date been examined in detail, but adverse and stressful housing conditions (e.g., mixing of groups) might impair the success of vaccinations. Therefore, we evaluated the influence of housing conditions on the immune response after two Improvac® vaccinations at an age of 12 and 22 weeks, respectively. Boars, immunocastrates and barrows (n = 48 each) were assigned to three different housing conditions (n = 36 enriched, n = 36 standard n = 72 repeated social mixing). Immune response was quantified by measuring GnRH-binding and its consequences for testosterone concentrations, development of the genital tract and boar taint. Growth performance was evaluated via average daily gain (ADG). GnRH-binding and testosterone levels revealed that immunocastration reliably suppressed testicular functions after the 2nd vaccination. Housing conditions did not modify testicular function but influenced ADG as animals under mixing grew slower than those under enriched conditions. Gonadal status had only a slight impact on ADG except in immunocastrates, which showed a temporarily higher ADG after the 2nd vaccination. The results show that immunocastration is a reliable procedure under different housing conditions and competitive in terms of growth performance.
Ključne besede: immunocastration, vaccination, Improvac, non-responder, immune response, housing conditions, surgical castration, boar taint, growth performance, genital tract
Objavljeno v DiRROS: 06.09.2022; Ogledov: 683; Prenosov: 243
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184. Priročnik za prepoznavanje tujerodnih drevesnih vrst v alpskem prostoruAjša Alagić, Bénédicte Baxerres, Frédéric Berger, Anja M. Bindewald, Sylvain Bouquet, Giuseppe Brundu, Debojyoti Chakraborty, Patricia Detry, Freya-Isabel Georges, Quentin Guillory, Janez Kermavnar, Lado Kutnar, Katharina Lapin, Aleksander Marinšek, Eric Mermin, Anja Müller-Meißner, Janine Oettel, Anica Simčič, Simon Zidar, 2022, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid Ključne besede: tujerodne drevesne vrste, Alpe, invazivne drevesne vrste
Objavljeno v DiRROS: 12.08.2022; Ogledov: 1335; Prenosov: 419
Celotno besedilo (19,49 MB) |
185. Identification handbook for non native tree species in the Alpine spaceAjša Alagić, Bénédicte Baxerres, Frédéric Berger, Anja M. Bindewald, Sylvain Bouquet, Giuseppe Brundu, Debojyoti Chakraborty, Patricia Detry, Freya-Isabel Georges, Quentin Guillory, Janez Kermavnar, Lado Kutnar, Katharina Lapin, Aleksander Marinšek, Eric Mermin, Anja Müller-Meißner, Janine Oettel, Anica Simčič, Simon Zidar, 2022, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid Ključne besede: non-native tree species, Alps, invasive alien species
Objavljeno v DiRROS: 12.08.2022; Ogledov: 652; Prenosov: 230
Celotno besedilo (17,57 MB) |
186. Rast in kakovost metasekvoje (Metasequoia glyptostroboides Hu et Cheng) v živem arhivu pri LjubljaniIza Petek, Gregor Božič, Dušan Jurc, Kristjan Jarni, Robert Brus, 2022, izvirni znanstveni članek Povzetek: Analizirali smo živi arhiv metasekvoje (Metasequoia glyptostroboides Hu et Cheng) na obrečnem rastišču ob Savi pri Ljubljani. Osnovan je bil v letih 1993 in 1994 s sadikami, vzgojenimi iz originalnih semen z območja na Kitajskem, kjer je bila vrsta opisana (klasično nahajališče oz. locus classicus). Drevesa v nasadu so bila prvič izmerjena spomladi leta 2000 in nato še leta 2006, spomladi v letu 2020 pa smo meritve ponovili. V starosti 30 let je povprečno drevo merilo v višino 20,7 m, povprečni premer koreninskega vratu na višini debla 0,1 m je znašal 72,3 cm, povprečni premer debla na prsni višini pa 51,4 cm. Ugotovili smo, da je pogostnost reproduktivnih organov, torej storžev in moških cvetov, povezana s premerom in višino drevesa. Užlebljenost debla je zelo variabilna in povezana s premerom, zelo variabilno je tudi zmanjšanje premera z višino. Povezave med omenjenimi znaki in lego drevesa v nasadu nismo ugotovili. Svetloba oziroma lega v nasadu ni edini dejavnik, ki vpliva na lastnosti posameznega drevesa, vzrok je tudi genetska variabilnost med drevesi.
Ključne besede: Metasequoia glyptostroboides, semenski nasad, rast, kakovost, Slovenija
Objavljeno v DiRROS: 11.08.2022; Ogledov: 739; Prenosov: 198
Celotno besedilo (694,92 KB) |
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188. Different wood anatomical and growth responses in European beech (Fagus sylvatica L.) at three forest sites in SloveniaDomen Arnič, Jožica Gričar, Jernej Jevšenak, Gregor Božič, Georg von Arx, Peter Prislan, 2021, izvirni znanstveni članek Povzetek: European beech (Fagus sylvatica L.) adapts to local growing conditions to enhance its performance. In response to variations in climatic conditions, beech trees adjust leaf phenology, cambial phenology, and wood formation patterns, which result in different treering widths (TRWs) and wood anatomy. Chronologies of tree ring width and vessel features [i.e., mean vessel area (MVA), vessel density (VD), and relative conductive area (RCTA)] were produced for the 1960%2016 period for three sites that differ in climatic regimes and spring leaf phenology (two early- and one late-flushing populations). These data were used to investigate long-term relationships between climatic conditions and anatomical features of four quarters of tree-rings at annual and intra-annual scales. In addition, we investigated how TRW and vessel features adjust in response to extreme weather events (i.e., summer drought). We found significant differences in TRW, VD, and RCTA among the selected sites. Precipitation and maximum temperature before and during the growing season were the most important climatic factors affecting TRW and vessel characteristics. We confirmed differences in climate-growth relationships between the selected sites, late flushing beech population at Idrija showing the least pronounced response to climate. MVA was the only vessel trait that showed no relationship with TRW or other vessel features. The relationship between MVA and climatic factors evaluated at intra-annual scale indicated that vessel area in the first quarter of tree-ring were mainly influenced by climatic conditions in the previous growing season, while vessel area in the second to fourth quarters of tree ring width was mainly influenced by maximum temperature and precipitation in the current growing season. When comparing wet and dry years, beech from all sites showed a similar response, with reduced TRW and changes in intra-annual variation in vessel area. Our findings suggest that changes in temperature and precipitation regimes as predicted by most climate change scenarios will affect tree-ring increments and wood structure in beech, yet the response between sites or populations may differ.
Ključne besede: Fagus sylvatica, wood anatomy, tracheograms, dendrochronology, intra specific plasticity
Objavljeno v DiRROS: 22.07.2022; Ogledov: 596; Prenosov: 394
Celotno besedilo (15,28 MB)
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189. River distance, stand basal area, and climatic conditions are the main drivers influencing lying deadwood in riparian forestsJanine Oettel, Martin Braun, Marcus Sallmannshofer, Maarten De Groot, Silvio Schueler, Charlotte Virgillito, Marjana Westergren, Gregor Božič, Laszlo Nagy, Srdjan Stojnić, Katharina Lapin, 2022, izvirni znanstveni članek Povzetek: Riparian forests are among the most diverse terrestrial ecosystems, yet their biodiversity is increasingly threatened by habitat degradation, climate change, river regulation and invasive species. We investigated deadwood, widely recognized as an indicator for forest biodiversity, in riparian forests of the Mura-Drava-Danube Transboundary Biosphere Reserve. The Biosphere Reserve is a conservation area that spans five countries and three rivers located in south-eastern Europe. In detail, we analyzed the drivers of lying deadwood volume, occurrence and decay related to floodplain type, silvicultural management, and climatic conditions using regression models. Lying deadwood occurrence and volume significantly decreased as distance from the river edge increased, indicating that river dynamics likely play a role in deadwood accumulation in riparian forests. Deadwood volume was also positively influenced by stand basal area, a parameter that can be directly addressed by silvicultural management. Deadwood decay was affected positively by temperature and negatively by precipitation, highlighting the importance of climatic conditions on decay progression. However, in order to draw more accurate conclusions about the drivers and dynamics of deadwood in riparian forests, further monitoring efforts that consider river flooding and flow regime, deadwood transport and saproxylic organism activity in addition to forest management and site conditions, are needed.
Ključne besede: alluvial forest, hardwood floodplain, deadwood decay, Mura-Drava-Danube transboundary biosphere, reserve, riparian area, softwood floodplain, UNESCO biosphere reserve, wetlands
Objavljeno v DiRROS: 18.07.2022; Ogledov: 544; Prenosov: 435
Celotno besedilo (1,55 MB)
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190. Delovni sklop 1.3 Pomen bolezni in škodljivcev GRM na poslovno uspešnost semenarn in drevesnic : Aktivnost 1.3.2 Problemi pri obnovi gozdov s sadnjo in setvijo ter vpliv bolezni in škodljivcev na izpad prihodka slovenskih semenarjev in drevesničarjevAndreja Kavčič, Hojka Kraigher, Gregor Božič, Barbara Piškur, Nikica Ogris, 2022, elaborat, predštudija, študija Ključne besede: domači projekti, gozdno semenarstvo in drevesničarstvo, bolezni, škodljivci, problemi, poslovna uspešnost
Objavljeno v DiRROS: 04.07.2022; Ogledov: 629; Prenosov: 229
Celotno besedilo (506,80 KB) |
