381. |
382. |
383. |
384. Multiplex quantification of 19 GM soybean lines using digital PCRAmadej Jelenčič, Dejan Štebih, Tina Demšar, David Dobnik, 2026, samostojni znanstveni sestavek ali poglavje v monografski publikaciji Povzetek: The European Union (EU) imposes strict regulations on the presence of genetically modified (GM) material in food and feed, requiring thorough testing of samples for various GM lines. Although traditional quantitative real-time PCR (qPCR) methods are sensitive and robust, they are not cost-effective for managing large numbers of GM events due to their limited multiplexing capabilities. Conversely, digital PCR (dPCR) is capable of robust quantitative multiplexing in addition to other benefits such as absolute quantification and better tolerance of PCR inhibitors. In this context, we present a protocol for multiplex quantification of 19 GM soybean lines using dPCR as an improvement over the currently used simplex qPCR approach. This method enables simple and robust quantification of common GM soybean lines with a relatively low number of reactions.
Ključne besede: genetically modified soybean, GMO, multiplexing, food and feed, food regulation, digital PCR (dPCR)
Objavljeno v DiRROS: 26.11.2025; Ogledov: 127; Prenosov: 40
 Povezava na datoteko |
385. Strain-controlled superconductivity in epitaxially grown thin films of ▫$1T-TaS_2$▫Yelyzaveta Chernolevska, Anže Mraz, Rok Venturini, Bojan Ambrožič, Tomaž Mertelj, Goran Dražić, Damjan Svetin, Damjan Vengust, Hsin-Chia Ho, Matjaž Spreitzer, Dragan Mihailović, 2025, izvirni znanstveni članek Ključne besede: transition metal dichalcogenides, thin films, molecular beam epitaxy
Objavljeno v DiRROS: 26.11.2025; Ogledov: 144; Prenosov: 80
 Celotno besedilo (3,77 MB)
Gradivo ima več datotek! Več... |
386. The European Information System on Forest Genetic Resources (EUFGIS): : a transnational tool for genetic conservation and policy supportMichele Bozzano, Valentina Barbiero, Egbert Beuker, Simone Mori, Mari Rusanen, Ivan Scotti, Milko Skofic, Marjana Westergren, 2025, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: forest genetic resources, information system, Europe
Objavljeno v DiRROS: 26.11.2025; Ogledov: 113; Prenosov: 55
Celotno besedilo (536,02 KB)
Gradivo ima več datotek! Več... |
387. Trans fatty acids in margarines and shortenings in the food supply in SloveniaHelena Abramovič, Rajko Vidrih, Emil Zlatić, Doris Kokalj Sinkovič, Matthias Schreiner, Katja Žmitek, Anita Kušar, Igor Pravst, 2018, izvirni znanstveni članek Objavljeno v DiRROS: 26.11.2025; Ogledov: 168; Prenosov: 88
Celotno besedilo (1,66 MB)
Gradivo ima več datotek! Več... |
388. Signalling network construction for modelling plant defence responseDragana Miljković, Tjaša Stare, Igor Mozetič, Vid Podpečan, Marko Petek, Kamil Witek, Marina Dermastia, Nada Lavrač, Kristina Gruden, 2012, izvirni znanstveni članek Objavljeno v DiRROS: 26.11.2025; Ogledov: 152; Prenosov: 84
Celotno besedilo (2,51 MB)
Gradivo ima več datotek! Več... |
389. In vitro evaluation of electrochemotherapy combined with sotorasib in pancreatic carcinoma cell lines harboring distinct kras mutationsTanja Jesenko, Maša Omerzel, Tina Živič, Gregor Serša, Maja Čemažar, 2025, izvirni znanstveni članek Povzetek: Pancreatic cancer is among the deadliest malignancies, with limited treatment options and poor prognosis. Novel strategies are therefore urgently needed. Sotorasib, a KRAS G12C-specific inhibitor, offers targeted treatment for a small subset of patients with this mutation. Electrochemotherapy (ECT), which enhances the cytotoxicity of chemotherapeutic agents through electroporation-induced membrane permeabilization, has shown promise in various tumor types, including deep-seated malignancies such as pancreatic cancer. Combining ECT with sotorasib may potentiate antitumor effects in KRAS G12C-mutated pancreatic cancer; however, preclinical data on such combinations are lacking. This proof-of-concept study evaluated the cytotoxic effects of ECT using bleomycin (BLM) or cisplatin (CDDP) in combination with sotorasib in KRAS G12C-mutated MIA PaCa-2 and KRAS G12D-mutated PANC-1 pancreatic cancer cell lines. ECT alone significantly reduced cell viability, particularly in MIA PaCa-2 cells, where electric pulses induced approximately 75% cell death. Combining ECT with sotorasib resulted in an additive effect on KRAS G12C-mutated MIA PaCa-2 cells, though no synergy was observed, likely due to the high intrinsic sensitivity to electric pulses. These results support the potential of combining physical and molecular therapies in a subset of pancreatic cancer patients and lay the groundwork for further in vivo studies to optimize treatment parameters and explore clinical translatability.
Ključne besede: bleomycin, cisplatin, electrochemotherapy, pancreatic cancer
Objavljeno v DiRROS: 26.11.2025; Ogledov: 100; Prenosov: 48
Celotno besedilo (743,73 KB)
Gradivo ima več datotek! Več... |
390. |
