1601. CD133/prominin1 is prognostic for GBM patient's survival, but inversely correlated with cysteine cathepsine' expression in gliobastoma derived spheroidsSeyed Yousef Ardebili, Irena Zajc, Boris Gole, Benito Campos, Christel Herold-Mende, Sara Drmota Prebil, Tamara Lah Turnšek, 2011, izvirni znanstveni članek Povzetek: Introduction. CD133 is a marker for a population of glioblastoma (GBM) and normal neural stem cells (NNSC). We aimed to reveal whether the migratory potential and differentiation of these stem cells is associated with CD133 expression and with cathepsin proteases (Cats). Materials and methods. The invasiveness of normal NNSC, GBM/CD133+ cell lines and GBM spheroids was evaluated in 3D collagen, as well as of U87-MG and normal astrocytes (NHA) grown in monolayers in 2D Matrigel. Expression of Cats B, L and S was measuredat mRNA and activity levels and their relation to invasiveness, to CD133 mRNA in 26 gliomas, and to the survival of these patients. Results. The average yield of CD133+ cells from GBM samples was 9.6%. Survival of patients with higher CD133 mRNA expression was significantly shorter (p< 0.005). Invasion, associated with proteolytic degradation of matrix, was higher in normal stem cells and GBM spheroids and cells than in isolated GBM CD133+ cells. In glioma samples, there was no correlation between CD133 mRNA expression and Cat mRNAs, but there was an inverse correlation with Cat activities. Conclusions. The study confirms CD133 as a prognostic marker for the survival of GBM patients. We demonstrated that NNSC have higher invasion potential and invade the collagen matrix in a mode different from that of GBM,initiating stem cell spheres. This result could have implications for the design of new therapeutics, including protease inhibitors that specifically target invasive tumour stem cells. Increased activity of cathepsins in CD133- cells suggests their role in the invasive behaviour of GBM. Objavljeno v DiRROS: 19.03.2024; Ogledov: 349; Prenosov: 104
Celotno besedilo (1,00 MB) |
1602. Microsatellite instability in colorectal cancerMatej Horvat, Borut Štabuc, 2011, pregledni znanstveni članek Povzetek: Background. Colorectal cancer (CRC) is the third most common cancer in the world. In 75% CRC develops sporadically, in 25% hereditary or as a consequenceof inflammatory bowel disease. CRC carcinogenesis develops over many years. The cause of CRC in 85% is chromosomal instability (CIN) and in 15% microsatellite instability (MSI-H), where hereditary nonpolyposis colorectal cancer (HNPCC) represents 10-20%. Microsatellite sequences (MS) arerepeated sequences of short stretches of DNA all over the genome. Microsatellite stability (MSS) means MS are the same in each cell of an individual, whereas microsatellite instability (MSI-H) means MS differ in normal and cancer cells of an individual. The cause of MSI-H is a damaged mismatch repair mechanism (MMR), with the most important MMR proteins being MSH2, MLH1 and MSH6. Conclusions. MSI-H seems to be an important prognostic factor in CRC and an important predictive factor of CRC chemotherapeutic treatment efficacy. Clinical trials conducted until now have shown contradictory findings in different chemotherapeutic settings, adjuvant and palliative; therefore MSI-H is going to be the object of the future research. The future of cancer treatment is in the individualized therapy based on molecular characteristics of the tumour, such as MSI-H in CRC. Objavljeno v DiRROS: 19.03.2024; Ogledov: 340; Prenosov: 164
Celotno besedilo (489,56 KB) Gradivo ima več datotek! Več... |
1603. Triple negative breast cancer : prognostic factors and survivalTanja Ovčariček, Snježana Frković-Grazio, Erika Matos, Barbara Možina, Simona Borštnar, 2011, izvirni znanstveni članek Povzetek: Background. Triple negative breast cancer (TNBC) is defined by a lack of expression of both estrogen (ER) and progesteron(PgR) receptors as well as human epidermal growth factor receptor 2 (HER2). Our retrospective analysis addressed prognostic factors for short- and long-term outcomes of patients (pts) with TNBC pts treated in routine clinical practice. Patient and methods.Our retrospective study included 269 TNBC treated at Institute of Oncology Ljubljana between March 2000 and December 2006. The collected data included patientsć, tumoursć and treatmentsć characteristics. The survival analyses were performed using the Kaplan-Meier method. The Cox proportional hazard model was used in the multivariate analysis. Results. The median age ofour patients was 55.3 yrs (23-88.5) and the median follow-up was 5.9 yrs (0.3-9.6). Six (2%) pts experienced local only, 79 (92%) pts distal recurrenceand 66 (24%) died. The predominant localisation of the first relapsewas in visceral organs (70.4%). The 5-year disease-free survival (DFS) for the entire group was 68.2% and the 5-year overall survival (OS) was 74.5%.We found a pattern of high recurrence rate in the first 3 years following the diagnosis and a clear decline in recurrence rate over the next 3years. In the univariate analysis age, nodal status, size and lymphovascular invasion (LVI) were found to have a significant impact on DFS as well as on OS. In the multivariate analysis only age (HR=1.79; 95%CI=1.14-2.82; p=0.012) and nodal status (HR=2.71; 95%CI=1.64-4.46; p<0.001) retained their independent prognostic value for DFS and for OS only the nodal status (HR=2.96; 95%CI=1.51-5.82; p=0.002). (Abstract truncated at 2000 characters) Objavljeno v DiRROS: 19.03.2024; Ogledov: 292; Prenosov: 121
Celotno besedilo (536,99 KB) |
1604. Image cytometric nuclear texture features in inoperable head and neck cancer : a pilot studyMargareta Strojan Fležar, Jaka Lavrenčak, Mario Žganec, Primož Strojan, 2011, izvirni znanstveni članek Povzetek: Background. Image cytometry can measure numerous nuclear features which could be considered a surrogate end-point marker of molecular genetic changes in a nucleus. The aim of the study was to analyze image cytometric nuclear featuresin paired samples of primary tumor and neck metastasis in patients with inoperable carcinoma of the head and neck. Materials and methods. Image cytometric analysis of cell suspensions prepared from primary tumor tissue andfine needle aspiration biopsy cell samples of neck metastases from 21 patients treated with concomitant radiochemotherapy was performed. Nuclear features were correlated with clinical characteristics and response to therapy. Results. Manifestation of distant metastases and new primaries was associated (p<0.05) with several chromatin characteristics from primary tumor cells, whereas the origin of index cancer and disease response in the neck wasrelated to those in the cells from metastases. Many nuclear features of primary tumors and metastases correlated with the TNM stage. Conclusions. A specific pattern of correlation between well-established prognostic indicatorsand nuclear features of samples from primary tumors and those from neck metastases was observed. Image cytometric nuclear features represent a promising candidate marker for recognition of biologically different tumor subgroups. Objavljeno v DiRROS: 19.03.2024; Ogledov: 291; Prenosov: 83
Celotno besedilo (427,02 KB) |
1605. Rople of [sup]18F-choline PET/CT in evaluation of patients with prostateMarina Hodolič, 2011, izvirni znanstveni članek Povzetek: Background. Choline presents a high affinity for maligna nt prostate tissue. It can be labelled with positron emitting 18f,and used for the evaluation of patients with prostate carcinoma by PET/CTimaging. The aim of this paper is tosummarise our experience with fluoromethylcholine ('8f-choline) PET/CTin patients with prosta te cancer. Methods. In 4 months we investigated the patients with histopathological (or cytological) confirmed prosta te cancer. Two observers evaluated the early and late 18f-choline PETimages in correlation with corresponding localising CT images and using the semiquantitative standard uptake value (SUV)calculation. Results. The 18f-choline PET/CT was made in 50 patients with prosta te cancer. There were 18 patients after radical prostatectomy and 32 without surgery.ln allpatients wiff1but surgery the pathological uptake was seen in the prostate. ln 14 (44 %) patients of this group there was evidence of metastatic spread in local or distant Iymph nodes and/or bones. In out of 18 patients after radical prostatectomy the local recurrence was detected in 6 patients (33%) and distant metastases were present in 2 patients (10%). Conclusions. 18f-choline PET/CTseems to be useful imaging modality in patients with prosta te carcinoma; it can demonstrate spread of the disease preoperatively and detect the local recurrence after radical prostatectomy. Objavljeno v DiRROS: 19.03.2024; Ogledov: 357; Prenosov: 209
Celotno besedilo (547,29 KB) |
1606. Influence of geology, hydrogeology, and climate on ground source heat pump distribution in Slovenia and selected European countriesSimona Adrinek, Mitja Janža, Rao Martand Singh, 2024, izvirni znanstveni članek Povzetek: Shallow geothermal energy (SGE) is a renewable energy that could contribute to the decarbonatization of the heating and cooling sector. SGE is predominantly harnessed through ground source heat pump (GSHP) systems. The choice of which type of GSHP system depends on various factors. Understanding these factors is crucial for optimizing the efficiency of GSHP systems and fostering their implementation. In this paper, we have analysed the spatial distribution of GSHPs in Slovenia. We identified 1073 groundwater and 1122 ground-coupled heat pump systems with a total heat pump capacity of almost 30 MW. We quantitatively assessed the influence of geological, hydrogeological, and climate conditions on their spatial distribution. Using the χ2 test and information value method, we identified hydrogeological conditions as the most influential factor for the GSHP systems’ spatial distribution. We also performed the spatial analysis of geological and hydrogeological data in 22 European countries, including Slovenia. We collected the reported numbers of installed GSHP units in 2020 and were able to distinguish the shares of groundwater and ground-coupled heat pump systems for 12 of these countries. The analysis showed that ground-coupled heat pumps predominate in most countries, even if the natural conditions are favourable for groundwater heat pumps. Ključne besede: shallow geothermal energy, renewable heating and cooling, ground-source heat pump, spatial distribution, natural condition Objavljeno v DiRROS: 19.03.2024; Ogledov: 331; Prenosov: 160
Celotno besedilo (15,39 MB) |
1607. |
1608. Distance formula for direct-co-direct product in the case of disconnected factorsAleksander Kelenc, Iztok Peterin, 2023, izvirni znanstveni članek Povzetek: Direct-co-direct product $G\circledast H$ of graphs $G$ and $H$ is a graph on the vertex set $V(G)\times V(H)$. Two vertices $(g,h)$ and $(g',h')$ are adjacent if $gg'\in E(G)$ and $hh'\in E(H)$ or $gg'\notin E(G)$ and $hh'\notin E(H)$. We show that if at most one factor of $G\circledast H$ is connected, then the distance between two vertices of $G\circledast H$ is bounded by three unless some small number of exceptions. All the exceptions are completely described which yields the distance formula. Ključne besede: direct-co-direct product, distance, eccentricity, disconnected graphs Objavljeno v DiRROS: 19.03.2024; Ogledov: 320; Prenosov: 134
Celotno besedilo (450,88 KB) Gradivo ima več datotek! Več... |
1609. |
1610. With computed tomography confirmed anterolateral left ventricular pseudoaneurysm in patient with dilatative alcoholic cardiomyopathyMitja Letonja, Marija Šantl-Letonja, 2011, izvirni znanstveni članek Povzetek: Background. Pseudoaneurysms are rare complications of myocardial infarction with propensity for rupture. There is still a challenge with which diagnostic imaging we performed a final diagnosis of pseudoaneurysm and differentiate it from true aneurysm what is clinically important due to the different treatment. Case report. We presented the unusual case of a 56-year-old man with signs of decompensated heart failure which had worsened a few months before hospitalization. We believed that during worsening of symptoms the patient suffered a silent myocardial infarction complicated by subacute free wall rupture which resulted into left ventricular pseudoaneurysm formation without tamponade. Echocardiography showed dilatativecardiomyopathy which was already present years before and a very rare location of the left ventricular pseudoaneurysm on the anterolateral part of the left ventricle. Pseudoaneurysm was confirmed with CT scan. Due to the severity of contractile dysfunction and no response in treatment for congestive heart failure the directive for the resection was tempered and the patient died due to the progressive heart failure and embolic phenomena. Conclusions. This report shows the importance of non-invasive imaging diagnostic evaluation of acute decompensated heart failure where echocardiography and chest X-ray are the first diagnostic steps. Based on those findings further imaging diagnostic steps must be performed such as CT scan in our case which finally confirms left ventricular pseudoaneurysm with dilatative cardiomyopathy. Objavljeno v DiRROS: 19.03.2024; Ogledov: 246; Prenosov: 123
Celotno besedilo (415,90 KB) Gradivo ima več datotek! Več... |