| Naslov: | Digital PCR outperforms quantitative real-time PCR for the detection and quantification of major periodontal pathobionts |
|---|
| Avtorji: | ID Munjaković, Haris (Avtor)
ID Povšič, Katja (Avtor)
ID Poljak, Mario (Avtor)
ID Seme, Katja (Avtor)
ID Gašperšič, Rok (Avtor)
ID Skubic, Lucijan (Avtor) |
|---|
| Datoteke: |  PDF - Predstavitvena datoteka, prenos (3,83 MB)
MD5: AF3DA315155973612B33B71EEBC82778
 URL - Izvorni URL, za dostop obiščite https://www.tandfonline.com/doi/10.1080/20002297.2025.2537439?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
|
|---|
| Jezik: | Angleški jezik |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: |  UKC LJ - Univerzitetni klinični center Ljubljana
|
|---|
| Povzetek: | Background: This study comparatively evaluated the analytical and diagnostic performance of a multiplex digital polymerase-chain reaction (dPCR) assay and a quantitative real-time PCR (qPCR) for the simultaneous detection and quantification of periodontal pathobionts: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum. Materials and methods: Subgingival plaque samples from 20 periodontitis patients and 20 periodontally healthy controls were analyzed. Several analytical parameters of the dPCR assay, optimized using DNA standards, were assessed versus qPCR: dynamic range linearity, precision, accuracy, prevalence, sensitivity, specificity, and concordance. The statistical analyses included Mann-Whitney U test, Wilcoxon test, McNemar's test, and Bland-Altman plots. Results: dPCR showed high linearity (R2 > 0.99) and lower intra-assay variability (median CV%: 4.5%) than qPCR (p = 0.020), with comparable accuracy and agreement. dPCR demonstrated superior sensitivity, detecting lower bacterial loads, particularly for P. gingivalis and A. actinomycetemcomitans. The Bland-Altman plots highlighted good agreement at medium/high loads but discrepancies at low concentrations (< 3 log10Geq/mL), resulting in qPCR false negatives and a 5-fold underestimation of the prevalence of A. actinomycetemcomitans in periodontitis patients. High concordance between the assays was observed for F. nucleatum across both study groups. Conclusions: dPCR outperformed qPCR for quantifying periodontal pathobionts and had superior sensitivity and precision, making it particularly effective in detecting low-level bacterial loads. |
|---|
| Ključne besede: | digital PCR, oral microbiology, periodontal disease, quantitative real-time PCR, subgingival plaque |
|---|
| Status publikacije: | Objavljeno |
|---|
| Verzija publikacije: | Objavljena publikacija |
|---|
| Leto izida: | 2025 |
|---|
| Št. strani: | str. 1-13 |
|---|
| Številčenje: | Vol. 17, iss. 1, [article no.] 2537439 |
|---|
| PID: | 20.500.12556/DiRROS-28991  |
|---|
| UDK: | 616.31 |
|---|
| ISSN pri članku: | 2000-2297 |
|---|
| DOI: | 10.1080/20002297.2025.2537439 |
|---|
| COBISS.SI-ID: | 245171203 |
|---|
| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne: 7. 8. 2025;
|
|---|
| Datum objave v DiRROS: | 15.04.2026 |
|---|
| Število ogledov: | 48 |
|---|
| Število prenosov: | 17 |
|---|
| Metapodatki: |    |
|---|
|
:
|
Kopiraj citat |
|---|
| | | | Objavi na: |  |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |
