| Naslov: | Phytoplankton diversity in the Northern Adriatic Sea : insights and inconsistencies from microscopy and metabarcoding |
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| Avtorji: | ID Montali, Giorgia (Avtor)
ID Neri, Francesca (Avtor)
ID Banchi, Elisa (Avtor)
ID Cerino, Federica (Avtor)
ID Turk Dermastia, Timotej (Avtor)
ID Francé, Janja (Avtor)
ID Mozetič, Patricija (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://doi.org/10.3390/biology15060487
 PDF - Predstavitvena datoteka, prenos (1,67 MB)
MD5: 8E281D81DBDB4F0954A123C4D950EF29
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | Phytoplankton plays a fundamental role in marine ecosystems and is widely used to assess environmental change. In this study, light microscopy and DNA metabarcoding approaches were used to investigate phytoplankton communities at three long-term monitoring sites in the northern Adriatic Sea. By combining the two approaches, more than 500 species were recorded. Metabarcoding detected substantially higher diversity, particularly among small and fragile organisms that are difficult to identify under a microscope, whereas microscopy was better for identifying larger species with distinctive morphology. Because species contain different amounts of genetic material, DNA data can overestimate or underestimate their true contribution to the community. Indeed, after applying correction factors that account for these differences, the DNA-based estimates became more consistent with the microscopy results for several phytoplankton groups. The differences among the samples were influenced more by the approach used than by the sampling location. Overall, combining these two approaches provides an accurate and robust picture of phytoplankton communities. In this way, it is possible to improve the reliability of marine biodiversity assessments and to support better environmental monitoring and management in coastal seas. |
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| Ključne besede: | phytoplankton, diversity, environmental DNA, Long-Term Ecological Research, amplicon sequencing, North Adriatic Sea |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 19.03.2026 |
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| Leto izida: | 2026 |
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| Št. strani: | str. 1-19 |
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| Številčenje: | Vol. 15, iss. 6, [article no.] 487 |
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| PID: | 20.500.12556/DiRROS-28612  |
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| UDK: | 574.5 |
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| ISSN pri članku: | 2079-7737 |
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| DOI: | 10.3390/biology15060487 |
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| COBISS.SI-ID: | 273040643 |
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| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 25. 3. 2026;
Soavtorji: Francesca Neri, Elisa Banchi, Federica Cerino, Timotej Turk Dermastia, Janja Francé, Patricija Mozetič, Angela Pelusi, Tiziana Romagnoli, Marika Ubaldi, Cecilia Totti and Stefano Accoroni;
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| Datum objave v DiRROS: | 25.03.2026 |
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| Število ogledov: | 29 |
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| Število prenosov: | 18 |
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| Metapodatki: |    |
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