| Naslov: | Genetic characterization of buckwheat accessions through genome-wide allele frequency fingerprints |
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| Avtorji: | ID Nay, Michelle M. (Avtor)
ID Byrne, Stephen L. (Avtor)
ID Pérez, Eduardo A. (Avtor)
ID Walter, Achim (Avtor)
ID Studer, Bruno (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://ojs.sazu.si/folia_bio_geo/article/view/7968
 PDF - Predstavitvena datoteka, prenos (4,04 MB)
MD5: D91C8C0DE12E0CD3D528CEFFCFFF9F3C
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  SAZU - Slovenska akademija znanosti in umetnosti
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| Povzetek: | Genomics-assisted breeding of buckwheat (Fagopyrum esculentum Moench) depends on robust genotyping methods. Genotyping by sequencing (GBS) has evolved as a flexible and cost-effective technique frequently used in plant breeding. Several GBS pipelines are available to genetically characterize single genotypes but these are not able to represent the genetic diversity of buckwheat accessions that are maintained as genetically heterogeneous, open-pollinating populations. Here we report the development of a GBS pipeline which, rather than reporting the state of bi-allelic single nucleotide polymorphisms (SNPs), resolves allele frequencies within populations on a genome-wide scale. These genome-wide allele frequency fingerprints (GWAFFs) from 100 pooled individual plants per accession were found to be highly reproducible and revealed the genetic similarity of 20 different buckwheat accessions analysed in our study. The GWAFFs cannot only be used as an efficient tool to precisely describe buckwheat breeding material, they also offer new opportunities to investigate the genetic diversity between different buckwheat accessions and establish variant databases for key material. Furthermore, GWAFFs provide the opportunity to associate allele frequencies to phenotypic traits and quality parameters that are most reliably described on population level. This is the key to practically implement powerful genomics-assisted breeding concepts such as marker-assisted selection and genomic selection in future breeding schemes of allogamous buckwheat. |
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| Ključne besede: | Buckwheat (Fagopyrum esculentum Moench), genotyping by sequencing (GBS), population genomics, genome-wide allele frequency fingerprints (GWAFFs) |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 05.05.2020 |
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| Leto izida: | 2020 |
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| Št. strani: | str. 17-23 |
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| Številčenje: | letn. 61, št. 1 |
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| PID: | 20.500.12556/DiRROS-25415  |
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| UDK: | 633.12:575 |
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| ISSN pri članku: | 1855-7996 |
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| DOI: | 10.3986/fbg0063 |
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| COBISS.SI-ID: | 212100099 |
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| Datum objave v DiRROS: | 20.01.2026 |
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| Število ogledov: | 126 |
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| Število prenosov: | 63 |
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| Metapodatki: |    |
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