| Naslov: | Correlation of oxidative stress biomarkers with activity of pediatric idiopathic nephrotic syndrome |
|---|
| Avtorji: | ID Kopač, Matjaž (Avtor)
ID Jerin, Aleš (Avtor)
ID Bohinc, Ema (Avtor)
ID Osredkar, Joško (Avtor) |
|---|
| Datoteke: |  PDF - Predstavitvena datoteka, prenos (1000,39 KB)
MD5: 90BA50A1CA2577F8CF7F8683BBF36DFB
 URL - Izvorni URL, za dostop obiščite https://www.mdpi.com/2227-9059/13/8/1984
|
|---|
| Jezik: | Angleški jezik |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: |  UKC LJ - Univerzitetni klinični center Ljubljana
|
|---|
| Povzetek: | Background/Objectives: This study investigated the correlation of oxidative stress biomarkers with the activity of idiopathic nephrotic syndrome (INS) in Slovenian children. Methods: In this prospective study, sequential plasma and urine samples from 20 children with INS in different phases of disease activity were taken: at first disease presentation or relapse (before glucocorticoid (GC) treatment), at time of remission achievement, and after discontinuation of GC treatment. This study measured oxidative stress biomarkers, such as 8-hydroxy-2′-deoxyguanosine (8-OHdG), hexanoyl-lysine (HEL) adduct, dityrosine (DiY), and 15-isoprostane F2t, using competitive enzyme-linked immunosorbent assay (ELISA) and assessed oxidative status using the FRAS 5 analytical system, which enables rapid photometric measurement of both oxidative and antioxidant capacity from biological fluids. Two complementary tests were performed: the d-ROMs test (derivatives of reactive oxygen metabolites) and the PAT (plasma antioxidant test). The oxidative stress index (OSI) was calculated as the ratio between them. Results: Concentrations of isoprostanes in urine were statistically significantly lower in patients at first disease presentation or relapse compared to time of remission achievement. Values of PAT test in serum were significantly highest after GC treatment. Values of d-ROMs test in serum were significantly lower at time of remission achievement compared to first disease presentation or relapse. Values of 8-OHdG, HEL, DiY (in plasma and urine), isoprostanes, and OSI in plasma did not statistically significantly differ in various phases of disease activity. Conclusions: Isoprostanes in urine and PAT in serum could serve as potential biomarkers of oxidative stress and disease activity in children with INS. |
|---|
| Ključne besede: | oxidative stress biomarkers, idiopathic nephrotic syndrome, children, oxidative stress index |
|---|
| Status publikacije: | Objavljeno |
|---|
| Verzija publikacije: | Objavljena publikacija |
|---|
| Leto izida: | 2025 |
|---|
| Št. strani: | str. 1-19 |
|---|
| Številčenje: | Vol. 13, iss. 8, [article no.] 1984 |
|---|
| PID: | 20.500.12556/DiRROS-24432  |
|---|
| UDK: | 616-053.2 |
|---|
| ISSN pri članku: | 2227-9059 |
|---|
| DOI: | 10.3390/biomedicines13081984 |
|---|
| COBISS.SI-ID: | 245827843 |
|---|
| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 18. 8. 2025;
|
|---|
| Datum objave v DiRROS: | 27.11.2025 |
|---|
| Število ogledov: | 112 |
|---|
| Število prenosov: | 46 |
|---|
| Metapodatki: |    |
|---|
|
:
|
Kopiraj citat |
|---|
| | | | Objavi na: |  |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |
