| Naslov: | Transcriptomics‑based analysis of acetate and propionate transport and metabolism in Yarrowia lipolytica |
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| Avtorji: | ID Žganjar, Mia, Institut "Jožef Stefan" (Avtor)
ID Sanz-Mata, David (Avtor)
ID Hancman, Urška (Avtor)
ID Čadež, Neža (Avtor)
ID Gostinčar, Cene (Avtor)
ID Gonzales-Fernandez, Cristina (Avtor)
ID Tomás Pejó, Elia (Avtor)
ID Petrovič, Uroš, Institut "Jožef Stefan" (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-025-02713-7
 PDF - Predstavitvena datoteka, prenos (3,97 MB)
MD5: FEE120542D7E2F2923F94C33CD218BE5
Opis: The assembled genome and raw expression data have been deposited at GeneBank under the BioProject accession number [PRJNA955139](https:/www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA955139).
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  IJS - Institut Jožef Stefan
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| Povzetek: | Background. Yarrowia lipolytica is a promising host for sustainable microbial oil production from waste-derived carboxylic acids such as acetate and propionate. Nonetheless, the molecular mechanisms underlying the metabolism and assimilation of these substrates, particularly under nitrogen limitation, are still not fully understood. Results. We conducted a multi-condition transcriptomic analysis of Y. lipolytica strain EXF-17398 under nitrogen-limiting conditions to investigate its transcriptional adaptation to acetate and propionate utilisation. Our results revealed distinct transcriptional responses associated with metabolic adaptation, including the coordinated regulation of Jen and Gpr carboxylate transporter families, suggesting a dual system for carboxylate uptake. JEN5 and GPR1 appear central to propionate and acetate utilisation, respectively. Our data suggest that propionate toxicity is mitigated through its conversion via the methylcitrate cycle and potentially the malonate semialdehyde pathway, preventing accumulation of cytotoxic propionyl-CoA in the cytosol. The upregulation of carnitine acyltransferases suggests active mitochondrial transport of acyl-CoAs, linking detoxification with energy metabolism. Under tested conditions, the de novo lipid synthesis was consistent with carbon overflow from acetyl-CoA and propionyl-CoA, supported by intracellular nitrogen recycling and redox balancing, independent of classical nitrogen regulatory pathways. Conclusions. These findings illustrate the capacity of Y. lipolytica to coordinate carbon and nitrogen metabolism during carboxylate utilisation, such as acetate and propionate, offering insights to guide the optimisation of microbial oil production from renewable feedstocks. |
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| Ključne besede: | propionate, transport, metabolism |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 17.06.2025 |
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| Datum sprejetja članka: | 19.10.2025 |
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| Datum objave: | 10.11.2025 |
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| Založnik: | Springer Nature |
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| Leto izida: | 2025 |
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| Št. strani: | str. 1-17 |
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| Številčenje: | Vol. 18, article no. 112 |
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| Izvor: | Švica |
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| PID: | 20.500.12556/DiRROS-24229  |
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| UDK: | 57 |
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| ISSN pri članku: | 2731-3654 |
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| DOI: | 10.1186/s13068-025-02713-7 |
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| COBISS.SI-ID: | 257066755 |
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| Avtorske pravice: | © The Author(s) 2025. |
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| Opomba: | Nasl. z nasl. zaslona;
Soavtorji iz Slovenije: Urška Hancman, Neža Čadež, Cene Gostinčar, Uroš Petrovič;
Opis vira z dne 13. 11. 2025;
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| Datum objave v DiRROS: | 21.11.2025 |
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| Število ogledov: | 173 |
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| Število prenosov: | 92 |
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| Metapodatki: |    |
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