| Naslov: | Cryo-EM structures of a protein pore reveal a cluster of cholesterol molecules and diverse roles of membrane lipids |
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| Avtorji: | ID Šolinc, Gašper (Avtor)
ID Srnko, Marija (Avtor)
ID Merzel, Franci (Avtor)
ID Crnković, Ana (Avtor)
ID Kozorog, Mirijam (Avtor)
ID Podobnik, Marjetka (Avtor)
ID Anderluh, Gregor (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://www.nature.com/articles/s41467-025-58334-z
 PDF - Predstavitvena datoteka, prenos (2,01 MB)
MD5: 65BE545EF338BB88EFEF724C22D8570C
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  KI - Kemijski inštitut
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| Povzetek: | The structure and function of membrane proteins depend on their interactions with lipids that constitute membranes. Actinoporins are α-pore-forming proteins that bind preferentially to sphingomyelin-containing membranes, where they oligomerize and form transmembrane pores. Through a comprehensive cryo-electron microscopic analysis of a pore formed by an actinoporin Fav from the coral Orbicella faveolata, we show that the octameric pore interacts with 112 lipids in the upper leaflet of the membrane, reveal the roles of lipids, and demonstrate that the actinoporin surface is suited for binding multiple receptor sphingomyelin molecules. When cholesterol is present in the membrane, it forms a cluster of four molecules associated with each protomer. Atomistic simulations support the structural data and reveal additional effects of the pore on the lipid membrane. These data reveal a complex network of protein-lipid and lipid-lipid interactions and an underrated role of lipids in the structure and function of transmembrane protein complexes. |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 01.01.2025 |
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| Leto izida: | 2025 |
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| Št. strani: | str. 1-11 |
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| Številčenje: | Vol. 16, article no. 2972 |
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| PID: | 20.500.12556/DiRROS-22223  |
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| UDK: | 577 |
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| ISSN pri članku: | 2041-1723 |
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| DOI: | 10.1038/s41467-025-58334-z |
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| COBISS.SI-ID: | 235400707 |
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| Avtorske pravice: | © The Author(s) 2025 |
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| Opomba: | Nasl. z nasl. zaslona;
Opis vira z dne 9. 5. 2025;
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| Datum objave v DiRROS: | 12.05.2025 |
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| Število ogledov: | 602 |
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| Število prenosov: | 302 |
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| Metapodatki: |    |
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