| Title: | One-step RT-droplet digital PCR : a breakthrough in the quantification of waterborne RNA viruses |
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| Authors: | ID Rački, Nejc (Author)
ID Morisset, Dany (Author)
ID Gutiérrez-Aguirre, Ion (Author)
ID Ravnikar, Maja (Author) |
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| Files: |  URL - Source URL, visit http://download.springer.com/static/pdf/380/art%253A10.1007%252Fs00216-013-7476-y.pdf?auth66=1386247888_e6b1a68707779a7e4b91476bbc18771b&ext=.pdf
 PDF - Presentation file, download (240,89 KB)
MD5: B1905B7D421538CFEF8BF8F64F4CAEFB
URL - Source URL, visit https://doi.org/10.1007/s00216-013-7476-y
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Water contamination by viruses has an increasing worldwide impact on human health, and has led to requirements for accurate and quantitative molecular tools. Here, we report the first one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This quantification method proved to be more precise and more tolerant to inhibitory substances than the benchmarking reverse-transcription real-time PCR (RT-qPCR), and needs no standard curve. This new tool is fully amenable for the quantification of viruses in the particularly low concentrations usually found in water samples.
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| Keywords: | waterborne virus, quantification, droplet digital PCR, real-time PCR, digital PCR |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 26.11.2013 |
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| Year of publishing: | 2014 |
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| Number of pages: | str. 661-667 |
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| Numbering: | Vol. 406, issue 3 |
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| PID: | 20.500.12556/DiRROS-21610  |
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| UDC: | 577.2 |
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| ISSN on article: | 1618-2642 |
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| DOI: | 10.1007/s00216-013-7476-y |
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| COBISS.SI-ID: | 2990415 |
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| Publication date in DiRROS: | 04.03.2025 |
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| Views: | 677 |
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| Downloads: | 458 |
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