20.500.12556/DiRROS-21610 One-step RT-droplet digital PCR a breakthrough in the quantification of waterborne RNA viruses Water contamination by viruses has an increasing worldwide impact on human health, and has led to requirements for accurate and quantitative molecular tools. Here, we report the first one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This quantification method proved to be more precise and more tolerant to inhibitory substances than the benchmarking reverse-transcription real-time PCR (RT-qPCR), and needs no standard curve. This new tool is fully amenable for the quantification of viruses in the particularly low concentrations usually found in water samples. waterborne virus quantification droplet digital PCR real-time PCR digital PCR true false true Angleški jezik Ni določen Neznano 2025-03-04 10:29:55 2025-03-04 10:29:55 2025-03-05 03:43:59 0000-00-00 00:00:00 2014 0 0 str. 661-667 issue 3 Vol. 406 2014 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2013-11-26 577.2 1618-2642 10.1007/s00216-013-7476-y 2990415 http://download.springer.com/static/pdf/380/art%253A10.1007%252Fs00216-013-7476-y.pdf?auth66=1386247888_e6b1a68707779a7e4b91476bbc18771b&ext=.pdf 1 3c17fcef-f8db-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30940 RAZ_Racki_Nejc_2014.pdf RAZ_Racki_Nejc_2014.pdf 1 B1905B7D421538CFEF8BF8F64F4CAEFB 0645a69e08305b129169cdf0637585aba1e70dea0dfee878ad0b36eb80ee4af5 54fcb073-f8db-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30941 https://doi.org/10.1007/s00216-013-7476-y 0 6e033636-f8db-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30942 Nacionalni inštitut za biologijo 0 0 0