| Naslov: | One-step RT-droplet digital PCR : a breakthrough in the quantification of waterborne RNA viruses |
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| Avtorji: | ID Rački, Nejc (Avtor)
ID Morisset, Dany (Avtor)
ID Gutiérrez-Aguirre, Ion (Avtor)
ID Ravnikar, Maja (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite http://download.springer.com/static/pdf/380/art%253A10.1007%252Fs00216-013-7476-y.pdf?auth66=1386247888_e6b1a68707779a7e4b91476bbc18771b&ext=.pdf
 PDF - Predstavitvena datoteka, prenos (240,89 KB)
MD5: B1905B7D421538CFEF8BF8F64F4CAEFB
URL - Izvorni URL, za dostop obiščite https://doi.org/10.1007/s00216-013-7476-y
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | Water contamination by viruses has an increasing worldwide impact on human health, and has led to requirements for accurate and quantitative molecular tools. Here, we report the first one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This quantification method proved to be more precise and more tolerant to inhibitory substances than the benchmarking reverse-transcription real-time PCR (RT-qPCR), and needs no standard curve. This new tool is fully amenable for the quantification of viruses in the particularly low concentrations usually found in water samples.
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| Ključne besede: | waterborne virus, quantification, droplet digital PCR, real-time PCR, digital PCR |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 26.11.2013 |
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| Leto izida: | 2014 |
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| Št. strani: | str. 661-667 |
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| Številčenje: | Vol. 406, issue 3 |
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| PID: | 20.500.12556/DiRROS-21610  |
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| UDK: | 577.2 |
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| ISSN pri članku: | 1618-2642 |
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| DOI: | 10.1007/s00216-013-7476-y |
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| COBISS.SI-ID: | 2990415 |
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| Datum objave v DiRROS: | 04.03.2025 |
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| Število ogledov: | 681 |
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| Število prenosov: | 459 |
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| Metapodatki: |    |
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