| Naslov: | Blood transcriptomics identifies multiple gene expression pathways associated with the clinical efficacy of Hymenoptera venom immunotherapy |
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| Avtorji: | ID Demšar Luzar, Ajda, Univerzitetna klinika za pljučne bolezni in alergijo Golnik (Avtor)
ID Korošec, Peter, Univerzitetna klinika za pljučne bolezni in alergijo Golnik (Avtor)
ID Košnik, Mitja, Univerzitetna klinika za pljučne bolezni in alergijo Golnik (Avtor)
ID Zidarn, Mihaela, Univerzitetna klinika za pljučne bolezni in alergijo Golnik (Avtor)
ID Rijavec, Matija, Univerzitetna klinika za pljučne bolezni in alergijo Golnik (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://www.mdpi.com/1422-0067/25/6/3499
 PDF - Predstavitvena datoteka, prenos (2,77 MB)
MD5: 45EC9328256111637FC1E553834F007E
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
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| Povzetek: | Allergen-specific venom immunotherapy (VIT) is a well-established therapy for Hymenoptera venom allergy (HVA). However, the precise mechanism underlying its clinical effect remains uncertain. Our study aimed to identify the molecular mechanisms associated with VIT efficiency. We prospectively included 19 patients with HVA undergoing VIT (sampled before the beginning of VIT, after reaching the maintenance dose, one year after finishing VIT, and after a sting challenge) and 9 healthy controls. RNA sequencing of whole blood was performed on an Illumina sequencing platform. Longitudinal transcriptomic profiling revealed the importance of the inhibition of the NFκB pathway and the downregulation of DUX4 transcripts for the early protection and induction of tolerance after finishing VIT. Furthermore, successful treatment was associated with inhibiting Th2, Th17, and macrophage alternative signalling pathways in synergy with the inhibition of the PPAR pathway and further silencing of the Th2 response. The immune system became activated when reaching the maintenance dose and was suppressed after finishing VIT. Finally, successful VIT restores the immune system’s balance to a state similar to that of healthy individuals. Our results underline the important role of the inhibition of four pathways in the clinical effect of VIT: Th2, Th17, NFκB, and macrophage signalling. Two biomarkers specific for successful VIT, regardless of the time of sampling, were C4BPA and RPS10-NUDT3 and should be further tested as potential biomarkers. |
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| Ključne besede: | Hymenoptera venom immunotherapy, longitudinal transcriptomic profiling, tolerance induction, successful venom immunotherapy |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 29.01.2024 |
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| Datum sprejetja članka: | 13.03.2024 |
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| Datum objave: | 20.03.2024 |
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| Kraj izida: | Basel |
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| Založnik: | MDPI |
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| Leto izida: | 2024 |
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| Št. strani: | str. 1-19 |
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| Številčenje: | Vol. 25, iss. 6, [article no.] 3499 |
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| Izvor: | International journal of molecular sciences |
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| PID: | 20.500.12556/DiRROS-21514  |
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| UDK: | 616-097 |
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| ISSN pri članku: | 1422-0067 |
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| DOI: | 10.3390/ijms25063499 |
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| COBISS.SI-ID: | 190409987 |
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| Avtorske pravice: | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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| Opomba: | Soavtorji: Peter Korošec , Mitja Košnik, Mihaela Zidarn in Matija Rijavec ;
Nasl. z nasl. zaslona;
Opis vira z dne 26. 3. 2024;
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| Datum objave v DiRROS: | 19.02.2025 |
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| Število ogledov: | 598 |
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| Število prenosov: | 384 |
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| Metapodatki: |    |
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