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Naslov:Multiplex basophil activation tests for allergy diagnosis : present and future applications
Avtorji:ID Koren, Ana, Univerzitetna klinika za pljučne bolezni in alergijo Golnik (Avtor)
ID Korošec, Peter, Univerzitetna klinika za pljučne bolezni in alergijo Golnik (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.frontiersin.org/journals/allergy/articles/10.3389/falgy.2024.1515843/full
 
.pdf PDF - Predstavitvena datoteka, prenos (44,02 MB)
MD5: 1185E412C035F1126305FA392F659836
 
Jezik:Angleški jezik
Tipologija:1.02 - Pregledni znanstveni članek
Organizacija:Logo UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
Povzetek:The basophil activation test (BAT) has become a major cellular in vitro test for evaluating the allergenic activity of specific IgEs. The impact of the BAT is due to the ability of blood basophil granulocytes to present IgE on the high-affinity FcεRI receptor and to mirror the mast cell response that elicits an acute allergic reaction. The BAT proved to be able to identify allergic patients at risk of reacting to a low dose of the allergen and/or developing life-threatening reactions and thus can significantly improve the current management of allergic patients. However, to improve the diagnostic utility for identifying the allergenic activity of different genuinely sensitizing allergens and lower the procedure and labour requirements, developing a multiplex BAT approach incorporating multiple allergen components would be highly anticipated. Recently, the novel multiplex BAT was described utilizing two major innovative steps. The first step was the fluorescent labeling of allergens. The second step was applying fluorescently labeled allergens in flow cytometry assessment to analyze the activation of basophil subpopulations gated according to the binding of different allergens or to evaluate the fluorescence intensity of multiple allergens on the surface of basophils. These novel cellular multiplex approaches will advance our understanding of IgE-mediated responses. Integration of multiplex BAT, in addition to multiplex IgE assays into practice, will personalize the measurement of allergenic IgE activity and can help estimate the likelihood of clinical relevance and risks for multiple allergens when testing individual allergic patients.
Ključne besede:allergy, diagnosis, basophil activation test, CD63, multiplex analysis
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Poslano v recenzijo:25.10.2024
Datum sprejetja članka:24.12.2024
Datum objave:14.01.2025
Založnik:Frontiers Media
Leto izida:2024
Št. strani:str. 1-11
Številčenje:Vol. 5
Izvor:Frontiers in allergy
PID:20.500.12556/DiRROS-21501 Novo okno
UDK:616-097
ISSN pri članku:2673-6101
DOI:10.3389/falgy.2024.1515843 Novo okno
COBISS.SI-ID:225121795 Novo okno
Avtorske pravice:© 2025 Koren and Korosec.
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 5. 2. 2025;
Datum objave v DiRROS:17.02.2025
Število ogledov:159
Število prenosov:108
Metapodatki:XML DC-XML DC-RDF
:
KOREN, Ana in KOROŠEC, Peter, 2024, Multiplex basophil activation tests for allergy diagnosis : present and future applications. Frontiers in allergy [na spletu]. 2024. Vol. 5, p. 1–11. [Dostopano 13 marec 2025]. DOI 10.3389/falgy.2024.1515843. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=21501
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Gradivo je del revije

Naslov:Frontiers in allergy
Skrajšan naslov:Front. allergy
Založnik:Frontiers Media
ISSN:2673-6101
COBISS.SI-ID:59156227 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P3-0360
Naslov:Celostna obravnava alergijskih bolezni in astme v Sloveniji: od epidemiologije do genetike

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:14.01.2025
Vezano na:Version of Record valid from 2025-01-14

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:alergije, diagnostika, test aktivacije bazofilcev, CD63, multipleksna analiza


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