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Naslov:Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates
Avtorji:ID Cámara, Elena (Avtor)
ID Olsson, Lisbeth (Avtor)
ID Zrimec, Jan (Avtor)
ID Zelezniak, Aleksej (Avtor)
ID Geijer, Cecilia (Avtor)
ID Nygård, Yvonne (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.sciencedirect.com/science/article/pii/S073497502200043X?via%3Dihub
 
.pdf PDF - Predstavitvena datoteka, prenos (3,76 MB)
MD5: 51770C6B28417F78677B1778B5D38DDB
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1016/j.biotechadv.2022.107947
 
Jezik:Angleški jezik
Tipologija:1.02 - Pregledni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:The use of renewable plant biomass, lignocellulose, to produce biofuels and biochemicals using microbial cell factories plays a fundamental role in the future bioeconomy. The development of cell factories capable of efficiently fermenting complex biomass streams will improve the cost-effectiveness of microbial conversion processes. At present, inhibitory compounds found in hydrolysates of lignocellulosic biomass substantially influence the performance of a cell factory and the economic feasibility of lignocellulosic biofuels and chemicals. Here, we present and statistically analyze data on Saccharomyces cerevisiae mutants engineered for altered tolerance towards the most common inhibitors found in lignocellulosic hydrolysates: acetic acid, formic acid, furans, and phenolic compounds. We collected data from 7971 experiments including single overexpression or deletion of 3955 unique genes. The mutants included in the analysis had been shown to display increased or decreased tolerance to individual inhibitors or combinations of inhibitors found in lignocellulosic hydrolysates. Moreover, the data included mutants grown on synthetic hydrolysates, in which inhibitors were added at concentrations that mimicked those of lignocellulosic hydrolysates. Genetic engineering aimed at improving inhibitor or hydrolysate tolerance was shown to alter the specific growth rate or length of the lag phase, cell viability, and vitality, block fermentation, and decrease product yield. Different aspects of strain engineering aimed at improving hydrolysate tolerance, such as choice of strain and experimental set-up are discussed and put in relation to their biological relevance. While successful genetic engineering is often strain and condition dependent, we highlight the conserved role of regulators, transporters, and detoxifying enzymes in inhibitor tolerance. The compiled meta-analysis can guide future engineering attempts and aid the development of more efficient cell factories for the conversion of lignocellulosic biomass.
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.08.2022
Leto izida:2022
Št. strani:str. 1-14
Številčenje:Vol. 57
PID:20.500.12556/DiRROS-20101 Novo okno
UDK:60:004
ISSN pri članku:0734-9750
DOI:10.1016/j.biotechadv.2022.107947 Novo okno
COBISS.SI-ID:120164867 Novo okno
Opomba:Št. članka: 107947;
Datum objave v DiRROS:05.08.2024
Število ogledov:467
Število prenosov:434
Metapodatki:XML DC-XML DC-RDF
:
CÁMARA, Elena, OLSSON, Lisbeth, ZRIMEC, Jan, ZELEZNIAK, Aleksej, GEIJER, Cecilia in NYGÅRD, Yvonne, 2022, Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates. Biotechnology advances [na spletu]. 2022. Vol. 57, p. 1–14. [Dostopano 6 april 2025]. DOI 10.1016/j.biotechadv.2022.107947. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=20101
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Gradivo je del revije

Naslov:Biotechnology advances
Skrajšan naslov:Biotechnol. adv.
Založnik:Pergamon
ISSN:0734-9750
COBISS.SI-ID:25101568 Novo okno

Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Številka projekta:P47509–1
Financer:Drugi - Drug financer ali več financerjev
Številka projekta:NNF19OC0055044
Naslov:Novo Nordisk Foundation

Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:lignocelulozna hidroliza, kvasovke, podatkovno rudarjenje, bioinformatika


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